Inhibitors | Comment | Organism | Structure |
---|---|---|---|
octopine | substrate inhibition above 2 mM, 50% product inhibition at 1.2 mM | Cerebratulus lacteus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
NAD+ | - |
Cerebratulus lacteus | |
0.028 | - |
NADH | - |
Cerebratulus lacteus | |
0.22 | - |
octopine | - |
Cerebratulus lacteus | |
0.5 | 1.5 | pyruvate | depending on concentration of the cosubstrates NADH and Arg | Cerebratulus lacteus | |
1.4 | 2.8 | Arg | dependent on concentration of the cosubstrates NADH and pyruvate | Cerebratulus lacteus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
ultracentrifugal studies | Cerebratulus lacteus |
41500 | - |
1 * 41500, SDS-PAGE | Cerebratulus lacteus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cerebratulus lacteus | - |
- |
- |
Pecten jacobaeus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cerebratulus lacteus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adductor | - |
Pecten jacobaeus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Cerebratulus lacteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Canavanine + pyruvate + NADH | L-canavanine, at 56% of the activity with L-Arg | Cerebratulus lacteus | N2-(D-1-Carboxyethyl)-canavanine + NAD+ + H2O | - |
? | |
Canavanine + pyruvate + NADH | L-canavanine, at 48% of the activity with L-Arg | Pecten jacobaeus | N2-(D-1-Carboxyethyl)-canavanine + NAD+ + H2O | - |
? | |
Homoarginine + pyruvate + NADH | at 22% of the activity with L-Arg | Pecten jacobaeus | N2-(D-1-Carboxyethyl)-homoarginine + NAD+ + H2O | - |
? | |
Homoarginine + pyruvate + NADH | at 13% of the activity with L-Arg | Cerebratulus lacteus | N2-(D-1-Carboxyethyl)-homoarginine + NAD+ + H2O | - |
? | |
L-Arg + 2-oxobutanoate + NADH | at 41% of the activity with L-Arg | Cerebratulus lacteus | N2-(D-2-Carboxypropyl)-L-Arg + NAD+ + H2O | - |
? | |
L-Arg + 2-oxobutanoate + NADH | at 28% of the activity with pyruvate | Pecten jacobaeus | N2-(D-2-Carboxypropyl)-L-Arg + NAD+ + H2O | - |
? | |
L-Arg + pyruvate + NADH | - |
Pecten jacobaeus | N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O | - |
? | |
L-Arg + pyruvate + NADH | r | Cerebratulus lacteus | N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O | - |
? | |
L-Lys + pyruvate + NADH | at 2% of the activity with L-Arg | Pecten jacobaeus | Lysopine + NAD+ + H2O | - |
? | |
L-Lys + pyruvate + NADH | at 1% of the activity with L-Arg | Cerebratulus lacteus | Lysopine + NAD+ + H2O | - |
? | |
Orn + pyruvate + NADH | at 2% of the activity with L-Arg | Cerebratulus lacteus | N2-(D-1-Carboxyethyl)-L-Orn + NAD+ + H2O | - |
? | |
Orn + pyruvate + NADH | at 4% of the activity with L-Arg | Pecten jacobaeus | N2-(D-1-Carboxyethyl)-L-Orn + NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41500, SDS-PAGE | Cerebratulus lacteus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Cerebratulus lacteus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | about 70% of maximal activity at pH 6 and pH 8 | Cerebratulus lacteus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Pecten jacobaeus | |
NADH | - |
Cerebratulus lacteus |