Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.1.11 extracted from

  • Birnberg, P.R.; Rao, S.S.; Lippincott, J.A.
    Octopine dehydrogenase of a Vinca rosea crown gall-tumor (1983), Phytochemistry, 22, 1345-1355.
No PubMed abstract available

General Stability

General Stability Organism
the apoenzyme is protected against inactivation by NADP+ Catharanthus roseus

Inhibitors

Inhibitors Comment Organism Structure
Methyl octopine
-
Catharanthus roseus
NADH substrate inhibition Catharanthus roseus
NADP+
-
Catharanthus roseus
NADPH substrate inhibition Catharanthus roseus
pyruvate substrate inhibition Catharanthus roseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information dependence of the Km-values for Arg and pyruvate on the concentration of the other substrates Catharanthus roseus
0.001 0.01 NADPH
-
Catharanthus roseus
0.015
-
NADP+
-
Catharanthus roseus
0.7
-
L-Orn with pyruvate and NADPH as cosubstrates Catharanthus roseus
1
-
oxaloacetate L-Lys, with pyruvate and NADPH as cosubstrates Catharanthus roseus
2.7
-
L-Gln with pyruvate and NADPH as cosubstrates Catharanthus roseus
3.9
-
L-canavanine with pyruvate and NADPH as cosubstrates Catharanthus roseus
5.1
-
L-Arg with pyruvate and NADPH as cosubstrate Catharanthus roseus
5.4
-
L-Cys with pyruvate and NADPH as cosubstrate Catharanthus roseus
22
-
L-citrulline with pyruvate and NADPH as cosubstrates Catharanthus roseus
22
-
L-citrulline L-Met, with pyruvate and NADPH as cosubstrate Catharanthus roseus
23
-
L-His with pyruvate and NADPH as cosubstrate Catharanthus roseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Catharanthus roseus the enzyme may synthesize lysopine, octopinic acid, and the corresponding Nalpha-derivatives of glutamine ?
-
?

Organism

Organism UniProt Comment Textmining
Catharanthus roseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
crown-gall tumor-tissue induced by Agrobacterium tumefaciens Catharanthus roseus

Source Tissue

Source Tissue Comment Organism Textmining
crown gall cell lines of Catharanthus roseus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Canavanine + pyruvate + NADPH at 56% of the activity with L-Arg Catharanthus roseus N2-(D-1-Carboxyethyl)-canavanine + NADP+ + H2O
-
?
Homoarginine + pyruvate + NADPH at 62% of the activity with L-Arg Catharanthus roseus N2-(D-1-Carboxyethyl)-homoarginine + NADP+ + H2O
-
?
L-Arg + 2-oxobutanoate + NADPH at 21% of the activity with pyruvate Catharanthus roseus N2-(D-2-Carboxypropyl)-L-Arg + NADP+ + H2O
-
?
L-Arg + glyoxylate + NADPH at 5% of the activity with pyruvate Catharanthus roseus ?
-
?
L-Arg + oxaloacetate + NADH
-
Catharanthus roseus ?
-
?
L-Arg + pyruvate + NADH
-
Catharanthus roseus N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
?
L-Arg + pyruvate + NADPH
-
Catharanthus roseus N2-(D-1-Carboxyethyl)-L-Arg + NADP+ + H2O
-
?
L-canavanine + pyruvate + NADPH + H+
-
Catharanthus roseus N2-(D-1-carboxyethyl)-canavanine + NADP+ + H2O
-
r
L-Citrulline + pyruvate + NADPH
-
Catharanthus roseus N2-(D-1-Carboxyethyl)-L-citrulline + NADP+ + H2O
-
?
L-Cys + pyruvate + NADPH at 27% of the activity with L-Arg Catharanthus roseus N2-(D-1-Carboxyethyl)-L-Cys + NADP+ + H2O
-
?
L-Gln + pyruvate + NADPH at 18% of the activity with L-Arg Catharanthus roseus N2-(D-1-Carboxyethyl)-L-Gln + NADP+ + H2O
-
?
L-His + pyruvate + NADPH at 78% of the activity with L-Arg Catharanthus roseus N2-(D-1-Carboxyethyl)-L-His + NADP+ + H2O
-
?
L-Lys + pyruvate + NADPH at 31% of the activity with L-Arg Catharanthus roseus Lysopine + NADP+ + H2O
-
?
L-Met + pyruvate + NADPH at 67% of the activity with L-Arg Catharanthus roseus N2-(D-1-Carboxyethyl)-L-Met + NADP+ + H2O
-
?
L-serine + pyruvate + NADPH at 25% of the activity with L-Arg Catharanthus roseus N2-(D-1-carboxyethyl)-L-Ser + NADP+ + H2O
-
?
additional information the enzyme may synthesize lysopine, octopinic acid, and the corresponding Nalpha-derivatives of glutamine Catharanthus roseus ?
-
?
Orn + pyruvate + NADPH at 30% of the activity with L-Arg Catharanthus roseus N2-(D-1-Carboxyethyl)-L-Orn + NADP+ + H2O
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
with Lys or Orn as substrate Catharanthus roseus
6 6.6 with Arg or His as substrate Catharanthus roseus
9.2
-
octopine oxidation Catharanthus roseus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8.5 4°C, stable. Loses most of its activity after 24 h at lower or higher pH-values Catharanthus roseus

Cofactor

Cofactor Comment Organism Structure
NADH
-
Catharanthus roseus
NADPH
-
Catharanthus roseus