General Stability | Organism |
---|---|
the apoenzyme is protected against inactivation by NADP+ | Catharanthus roseus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Methyl octopine | - |
Catharanthus roseus | |
NADH | substrate inhibition | Catharanthus roseus | |
NADP+ | - |
Catharanthus roseus | |
NADPH | substrate inhibition | Catharanthus roseus | |
pyruvate | substrate inhibition | Catharanthus roseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | dependence of the Km-values for Arg and pyruvate on the concentration of the other substrates | Catharanthus roseus | |
0.001 | 0.01 | NADPH | - |
Catharanthus roseus | |
0.015 | - |
NADP+ | - |
Catharanthus roseus | |
0.7 | - |
L-Orn | with pyruvate and NADPH as cosubstrates | Catharanthus roseus | |
1 | - |
oxaloacetate | L-Lys, with pyruvate and NADPH as cosubstrates | Catharanthus roseus | |
2.7 | - |
L-Gln | with pyruvate and NADPH as cosubstrates | Catharanthus roseus | |
3.9 | - |
L-canavanine | with pyruvate and NADPH as cosubstrates | Catharanthus roseus | |
5.1 | - |
L-Arg | with pyruvate and NADPH as cosubstrate | Catharanthus roseus | |
5.4 | - |
L-Cys | with pyruvate and NADPH as cosubstrate | Catharanthus roseus | |
22 | - |
L-citrulline | with pyruvate and NADPH as cosubstrates | Catharanthus roseus | |
22 | - |
L-citrulline | L-Met, with pyruvate and NADPH as cosubstrate | Catharanthus roseus | |
23 | - |
L-His | with pyruvate and NADPH as cosubstrate | Catharanthus roseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Catharanthus roseus | the enzyme may synthesize lysopine, octopinic acid, and the corresponding Nalpha-derivatives of glutamine | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
crown-gall tumor-tissue induced by Agrobacterium tumefaciens | Catharanthus roseus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
crown gall | cell lines of | Catharanthus roseus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Canavanine + pyruvate + NADPH | at 56% of the activity with L-Arg | Catharanthus roseus | N2-(D-1-Carboxyethyl)-canavanine + NADP+ + H2O | - |
? | |
Homoarginine + pyruvate + NADPH | at 62% of the activity with L-Arg | Catharanthus roseus | N2-(D-1-Carboxyethyl)-homoarginine + NADP+ + H2O | - |
? | |
L-Arg + 2-oxobutanoate + NADPH | at 21% of the activity with pyruvate | Catharanthus roseus | N2-(D-2-Carboxypropyl)-L-Arg + NADP+ + H2O | - |
? | |
L-Arg + glyoxylate + NADPH | at 5% of the activity with pyruvate | Catharanthus roseus | ? | - |
? | |
L-Arg + oxaloacetate + NADH | - |
Catharanthus roseus | ? | - |
? | |
L-Arg + pyruvate + NADH | - |
Catharanthus roseus | N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O | - |
? | |
L-Arg + pyruvate + NADPH | - |
Catharanthus roseus | N2-(D-1-Carboxyethyl)-L-Arg + NADP+ + H2O | - |
? | |
L-canavanine + pyruvate + NADPH + H+ | - |
Catharanthus roseus | N2-(D-1-carboxyethyl)-canavanine + NADP+ + H2O | - |
r | |
L-Citrulline + pyruvate + NADPH | - |
Catharanthus roseus | N2-(D-1-Carboxyethyl)-L-citrulline + NADP+ + H2O | - |
? | |
L-Cys + pyruvate + NADPH | at 27% of the activity with L-Arg | Catharanthus roseus | N2-(D-1-Carboxyethyl)-L-Cys + NADP+ + H2O | - |
? | |
L-Gln + pyruvate + NADPH | at 18% of the activity with L-Arg | Catharanthus roseus | N2-(D-1-Carboxyethyl)-L-Gln + NADP+ + H2O | - |
? | |
L-His + pyruvate + NADPH | at 78% of the activity with L-Arg | Catharanthus roseus | N2-(D-1-Carboxyethyl)-L-His + NADP+ + H2O | - |
? | |
L-Lys + pyruvate + NADPH | at 31% of the activity with L-Arg | Catharanthus roseus | Lysopine + NADP+ + H2O | - |
? | |
L-Met + pyruvate + NADPH | at 67% of the activity with L-Arg | Catharanthus roseus | N2-(D-1-Carboxyethyl)-L-Met + NADP+ + H2O | - |
? | |
L-serine + pyruvate + NADPH | at 25% of the activity with L-Arg | Catharanthus roseus | N2-(D-1-carboxyethyl)-L-Ser + NADP+ + H2O | - |
? | |
additional information | the enzyme may synthesize lysopine, octopinic acid, and the corresponding Nalpha-derivatives of glutamine | Catharanthus roseus | ? | - |
? | |
Orn + pyruvate + NADPH | at 30% of the activity with L-Arg | Catharanthus roseus | N2-(D-1-Carboxyethyl)-L-Orn + NADP+ + H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
with Lys or Orn as substrate | Catharanthus roseus |
6 | 6.6 | with Arg or His as substrate | Catharanthus roseus |
9.2 | - |
octopine oxidation | Catharanthus roseus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | 4°C, stable. Loses most of its activity after 24 h at lower or higher pH-values | Catharanthus roseus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Catharanthus roseus | |
NADPH | - |
Catharanthus roseus |