1.4.1.20: phenylalanine dehydrogenase
This is an abbreviated version!
For detailed information about phenylalanine dehydrogenase, go to the full flat file.
Word Map on EC 1.4.1.20
Reaction
Synonyms
dehydrogenase, phenylalanine, L-PheDH, L-phenylalanine dehydrogenase, L-phenylalanine:NAD+ oxidoreductase, deaminating, PDH, PHD, PheDH, phenylalanine dehydrogenase, recombinant PheDH, recombinant phenylalanine dehydrogenase
ECTree
1.4.1.20 phenylalanine dehydrogenaseAdvanced search results
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results (Temperature Stability
Temperature Stability on EC 1.4.1.20 - phenylalanine dehydrogenase
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TEMPERATURE STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
30 - 45
-
native enzyme, 15 min, 96% activity remaining at pH 7.5, no activity remaining at pH 10.4
30 - 55
after incubation at 30-55°C for 1 h under pH 10.0, the enzyme retains over 60% of its maximal activity while it retains about no activity at 55°C
37
-
t1/2: 48 min, in absence of substrate or coenzyme. t1/2: 2000 min, in presence of 10 mM L-Leu and 1.0 mM NAD+
37 - 60
Halalkalibacterium halodurans
2 h incubation, pH 7.0, enzyme retaines approximately 80% of maximum activity
40
45 - 61
-
the optimum temperature for the glycosylated enzyme is increased by 15°C and its thermostability is improved by about 6°C over 10 min incubation, the conjugate is more resistant to thermal inactivation at different temperatures, ranging from 45 to 60°C, the T50 value is increased from 54 to 61°C for PheDH after glycosidation
50
53
54
55
58
-
pH 7.0-9.5, 60 min, chimeric enzyme, loss of activity above
60
65 - 70
70
additional information
-
the enzyme glycosylated with mono-6-amino-6-deoxy-beta-cyclodextrin and mono-6-(5-carboxypentane-1-carboxamidoyl)-6-deoxy-beta-cyclodextrin contains about 18 mol and 15 mol oligosaccharide per mol of protein and retains 60% and 81% of the initial activity, respectively. the thermostability profile of the enzyme is improved abd its resistance to thermal inactivation at different temperatures ranging from 45°C to 60°C is noticeably increased after glycosylation
50
-
60 min, mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q loses 50% of its activity
53
-
phenylpyruvate destabilizes the activity, 1 M sorbitol or 1 M glycerol stabilize
60
-
wild type PDH retains most of its activity after 1 h at 60°C whereas modified PDH which contains two amino acid changes at the C-terminus and a 12 amino acid extension of the C-terminus loses all activity after 1h at 60°C
