Application | Comment | Organism |
---|---|---|
diagnostics | the immobilized enzyme is useful for establishing an analytical spectrophotometric determination method of L-Phe in the diagnostics of phenyketonuria, an inborn error of amino acid metabolism in which the conversion of L-phenylalanine to L-tyrosine is impaired and can cause profound mental retardation if not detected and treated soon after birth. Early quantitative measurement of the plasma L-Phe is essential for the diagnosis of phenylketonuria and the control of dietary therapy of the patients. The conversion efficiency of the reactor with immobillized enzyme is 100% in the range of 0.005-0.6 mM Phe at 9 mM NAD+ with a total flow rate of 0.1 mL/min. The reactor is used for the analyses of 30 samples each for 3 h per day. The half-life period of the reactor is 15 days, method evaluation, overview | Sporosarcina ureae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the enzyme is modified initially with 2-amino-4,6-dichloro-s-triazine, and immobilized by hexamethylenediamine and glutaraldehyde. The highest activity of immobilized PheDH is determined as 95.75 U/g support with 56% retained activity. The optimum pH value of immobilized L-PheDH was shifted from pH 10.4 to pH 11.0. pH and thermal stability of the immobilized L-PheDH are improved compared to the native enzyme | Sporosarcina ureae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
L-phenylalanine | pH 10.4, 30°C, immobilized enzyme | Sporosarcina ureae | |
0.052 | - |
L-phenylalanine | pH 10.4, 30°C, native enzyme | Sporosarcina ureae | |
0.063 | - |
L-phenylalanine | pH 10.4, 25°C, immobilized enzyme | Sporosarcina ureae | |
0.076 | - |
L-phenylalanine | pH 10.4, 30°C, immobilized enzyme | Sporosarcina ureae | |
0.118 | - |
L-phenylalanine | pH 10.4, 25°C, native enzyme | Sporosarcina ureae | |
0.128 | - |
NAD+ | pH 10.4, 25°C, immobilized enzyme | Sporosarcina ureae | |
0.167 | - |
L-phenylalanine | pH 10.4, 30°C, native enzyme | Sporosarcina ureae | |
0.234 | - |
NAD+ | pH 10.4, 25°C, native enzyme | Sporosarcina ureae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + H2O + NAD+ | Sporosarcina ureae | - |
phenylpyruvate + NH3 + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sporosarcina ureae | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.1 | - |
native enzyme, pH 10.4, 30°C | Sporosarcina ureae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + H2O + NAD+ | - |
Sporosarcina ureae | phenylpyruvate + NH3 + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
L-PheDH | - |
Sporosarcina ureae |
PheDH | - |
Sporosarcina ureae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
native enzyme | Sporosarcina ureae |
45 | 55 | immobilized enzyme | Sporosarcina ureae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 45 | native enzyme, 15 min, 96% activity remaining at pH 7.5, no activity remaining at pH 10.4 | Sporosarcina ureae |
40 | - |
native enzyme, 10 min, 75% activity remaining at pH 9.0 | Sporosarcina ureae |
50 | - |
native enzyme, 10 min, no activity remaining at pH 9.0 | Sporosarcina ureae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.37 | - |
L-phenylalanine | pH 10.4, 25°C, native enzyme | Sporosarcina ureae | |
8.55 | - |
L-phenylalanine | pH 10.4, 30°C, native enzyme | Sporosarcina ureae | |
28.71 | - |
L-phenylalanine | pH 10.4, 25°C, immobilized enzyme | Sporosarcina ureae | |
49.56 | - |
L-phenylalanine | pH 10.4, 30°C, immobilized enzyme | Sporosarcina ureae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.4 | - |
native enzyme | Sporosarcina ureae |
11 | - |
immobilized enzyme | Sporosarcina ureae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
native enzyme, 15 min, 96% activity remaining at 30-45°C | Sporosarcina ureae |
9 | - |
native enzyme, 10 min, 75% activity remaining at 40°C, no activity at 50°C | Sporosarcina ureae |
10.4 | - |
native enzyme, 15 min, no activity remaining at 30-45°C | Sporosarcina ureae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Sporosarcina ureae | |
NADH | - |
Sporosarcina ureae |
General Information | Comment | Organism |
---|---|---|
malfunction | an inborn error of amino acid metabolism, in which the conversion of L-phenylalanine to L-tyrosine is impaired and can cause profound mental retardation if not detected and treated soon after birth, is phenylketonuria, a disease due to an autosomal recessive inheritance that codes for a type of phenylalanine hydroxylase with reduced enzymatic activity, resulting in high levels of L-Phe in inborn fluid and phenylpyruvate in urine | Sporosarcina ureae |