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26
2-oxo-4-methylpentanoate
-
wild-type enzyme
37
2-oxo-4-methylthiobutanoate
-
wild-type enzyme
79
2-Oxohexanoate
-
wild-type enzyme
57
2-Oxoisohexanoate
-
wild-type enzyme
82
2-oxopentanoate
-
wild-type enzyme
27
4-methyl-2-oxopentanoate
-
25°C, pH 10.4
0.72
4-phenyl-2-butanone
mutant enzyme K66Q/S149G/N262C, at pH 9.6 and 30°C
27
alpha-keto-gamma-methiobutyrate
-
25°C, pH 10.4
51
alpha-Ketocaproate
-
25°C, pH 10.4
33
alpha-ketovalerate
-
25°C, pH 10.4
7.2
DL-alpha-keto-beta-methyl-n-valerate
-
25°C, pH 10.4
0.5 - 1.82
DL-propargylglycine
0.14
L-isoleucine
-
25°C, pH 10.4
0.7
L-leucine
-
25°C, pH 10.4
1.2
L-methionine
-
25°C, pH 10.4
0.608 - 68.01
L-phenylalanine
0.91
L-valine
-
25°C, pH 10.4
90
p-hydroxy-phenylpyruvate
-
25°C, pH 10.4
0.7
phenylacetone
mutant enzyme K66Q/S149G/N262C, at pH 9.6 and 30°C
additional information
additional information
-
turnover numbers of wild-type and mutant enzymes
-
0.5
DL-propargylglycine
-
mutations K224Q/L283F/E313G
0.57
DL-propargylglycine
-
mutations G124A/E313N
0.59
DL-propargylglycine
-
mutations Q18H/I336F/E313G
0.69
DL-propargylglycine
-
mutation G124A
0.722
DL-propargylglycine
-
wild type enzyme of Bacillus sphaericus
0.759
DL-propargylglycine
-
mutations F110Y/G124C/G293A
0.92
DL-propargylglycine
-
mutations G124A/E313G
1.02
DL-propargylglycine
-
mutations Q18H/I336F
1.07
DL-propargylglycine
-
mutation E313G
1.15
DL-propargylglycine
-
mutations K224Q/L283F
1.3
DL-propargylglycine
-
mutations D209G/Q18H/I336F
1.42
DL-propargylglycine
-
mutations V33A/A206D/L283F
1.49
DL-propargylglycine
-
mutations V135I/I308N/Q363H
1.82
DL-propargylglycine
-
mutation D209G
0.1
L-ethionine
-
mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q
1.1
L-ethionine
-
wild-type enzyme
0.24
L-Ile
-
wild-type enzyme
0.28
L-Ile
-
mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q
0.21
L-Leu
-
mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q
3.6
L-Leu
-
wild-type enzyme
0.16
L-Met
-
mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q
0.84
L-Met
-
wild-type enzyme
0.17
L-norleucine
-
mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q
1.9
L-norleucine
-
wild-type enzyme
3.2
L-norleucine
-
25°C, pH 10.4
0.15
L-norvaline
-
mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q
0.59
L-norvaline
-
wild-type enzyme
1.4
L-norvaline
-
25°C, pH 10.4
0.75
L-Phe
-
mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q
45
L-Phe
-
wild-type enzyme
714
L-Phe
-
25°C, pH 10.4, dextran-modified enzyme
816
L-Phe
-
25°C, pH 10.4, native enzyme
0.608
L-phenylalanine
-
mutations V135I/I308N/Q363H
0.91
L-phenylalanine
-
mutations Q18H/I336F/E313G
1.09
L-phenylalanine
-
mutations K224Q/L283F/E313G
1.12
L-phenylalanine
-
mutations F110Y/G124C/G293A
2.6
L-phenylalanine
-
mutation G124A
3 - 6
L-phenylalanine
mutant V144L, pH 10.4, 25°C
3.07
L-phenylalanine
-
mutations G124A/E313N
3.16
L-phenylalanine
-
mutations K224Q/L283F
4.08
L-phenylalanine
-
mutations D209G/Q18H/I336F
4.66
L-phenylalanine
-
mutations G124A/E313G
5.37
L-phenylalanine
-
pH 10.4, 25°C, native enzyme
5.8
L-phenylalanine
-
mutations V33A/A206D/L283F
6.56
L-phenylalanine
-
mutations Q18H/I336F
8.55
L-phenylalanine
-
pH 10.4, 30°C, native enzyme
13.5
L-phenylalanine
mutant V144D, pH 10.4, 25°C
23
L-phenylalanine
mutant V144N, pH 10.4, 25°C
28.71
L-phenylalanine
-
pH 10.4, 25°C, immobilized enzyme
32.1
L-phenylalanine
-
mutation E313G
49.56
L-phenylalanine
-
pH 10.4, 30°C, immobilized enzyme
50
L-phenylalanine
wild-type, pH 10.4, 25°C
56.1
L-phenylalanine
-
mutation D209G
57.1
L-phenylalanine
-
wild type enzyme of Bacillus sphaericus
68.01
L-phenylalanine
-
25°C, pH 10.4
26
L-tyrosine
mutant V144L, pH 10.4, 25°C
28.5
L-tyrosine
mutant V144D, pH 10.4, 25°C
57
L-tyrosine
mutant V144N, pH 10.4, 25°C
58
L-tyrosine
wild-type, pH 10.4, 25°C
68
L-tyrosine
-
25°C, pH 10.4
91
phenylpyruvate
-
wild-type enzyme
113
phenylpyruvate
-
25°C, pH 10.4