1.4.1.20: phenylalanine dehydrogenase

This is an abbreviated version!
For detailed information about phenylalanine dehydrogenase, go to the full flat file.

Word Map on EC 1.4.1.20

Reaction

L-phenylalanine
+
H2O
+
NAD+
=
phenylpyruvate
+
NH3
+
NADH
+
H+

Synonyms

dehydrogenase, phenylalanine, L-PheDH, L-phenylalanine dehydrogenase, L-phenylalanine:NAD+ oxidoreductase, deaminating, PDH, PHD, PheDH, phenylalanine dehydrogenase, recombinant PheDH, recombinant phenylalanine dehydrogenase

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.20 phenylalanine dehydrogenase

Reference

Reference on EC 1.4.1.20 - phenylalanine dehydrogenase

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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Okazaki, N.; Hibino, Y.; Asano, Y.; Ohmori, M.; Numao, N.; Kondo, K.
Cloning and nucleotide sequencing of phenylalanine dehydrogenase gene of Bacillus sphaericus
Gene
63
337-341
1988
Lysinibacillus sphaericus, Lysinibacillus sphaericus SCRC-79a
Manually annotated by BRENDA team
Asano, Y.; Nakazawa, A.
Crystallization of phenylalanine dehydrogenase from Sporosarcina urea
Agric. Biol. Chem.
49
3631-3632
1985
Sporosarcina ureae
-
Manually annotated by BRENDA team
Asano, Y.; Nakazawa, A.; Endo, K.
Novel phenylalanine dehydrogenases from Sporosarcina ureae and Bacillus sphaericus. Purification and characterization
J. Biol. Chem.
262
10346-10354
1987
Lysinibacillus sphaericus, Sporosarcina ureae
Manually annotated by BRENDA team
De Boer, L.; Van Rijssel, M.; Euverink, G.J.; Dijkhuizen, L.
Purification, characterization and regulation of a monomeric L-phenylalanine dehydrogenase from the facultative methylotroph Nocardia sp. 239
Arch. Microbiol.
153
12-18
1989
Nocardia sp., Nocardia sp. 239
-
Manually annotated by BRENDA team
Misono, H.; Yonezawa, J.; Nagata, S.; Nagasaki, S.
Purification and characterization of a dimeric phenylalanine dehydrogenase from Rhodococcus maris K-18
J. Bacteriol.
171
30-36
1989
Dietzia maris, Dietzia maris K-18
Manually annotated by BRENDA team
Asano, Y.; Endo, K.; Nakazawa, A.; Hibino, Y.; Okazaki, N.; Ohmori, M.; Numao, N.; Kondo, K.
Bacillus phenylalanine dehydrogenase produced in Escherichia coli - Its purification and application to L-phenylalanine synthesis
Agric. Biol. Chem.
51
2621-2623
1987
Lysinibacillus sphaericus, Pediococcus acidilactici
-
Manually annotated by BRENDA team
Asano, Y.; Nakazawa, A.; Endo, K.; Hibino, Y.; Ohmori, M.; Numao, N.; Kondo, K.
Phenylalanine dehydrogenase of Bacillus badius. Purification, characterization and gene cloning [published erratum appears in Eur J Biochem 1988 Jan 1;170(3):667]
Eur. J. Biochem.
168
153-159
1987
Bacillus badius
Manually annotated by BRENDA team
Hummel, W.; Weiss, N.; Kula, M.R.
Isolation and characterization of a bacterium possessing L-phenylalanine dehydrogenase activity
Arch. Microbiol.
137
47-52
1984
Brevibacterium sp.
-
Manually annotated by BRENDA team
Hummel, W.; Schuette, H.; Schmidt, E.; Wandrey, C.; Kula, M.R.
Isolation of L-phenylalanine dehydrogenase from Rhodococcus sp. M4 and its application for the production of L-phenylalanine
Appl. Microbiol. Biotechnol.
26
409-416
1987
Rhodococcus sp., Rhodococcus sp. M4
-
Manually annotated by BRENDA team
Hummel, W.; Schuette, H.; Kula, M.R.
Enzymatic determination of L-phenylalanine and phenylpyruvate with L-phenylalanine dehydrogenase
Anal. Biochem.
170
397-401
1988
Brevibacterium sp.
Manually annotated by BRENDA team
Campagna, R.; Bueckmann, A.F.
Comparison of the production of intracellular L-phenylalanine dehydrogenase by Rhodococcus species M4 and Sporosarcina urea at 50 liter scale
Appl. Microbiol. Biotechnol.
26
417-421
1987
Rhodococcus sp., Rhodococcus sp. M4, Sporosarcina ureae
-
Manually annotated by BRENDA team
Wendel, U.; Hummel, W.; Langenbeck, U.
Monitoring of phenylketonuria: a colorimetric method for the determination of plasma phenylalanine using L-phenylalanine dehydrogenase
Anal. Biochem.
180
91-94
1989
Rhodococcus sp., Rhodococcus sp. M4
Manually annotated by BRENDA team
Hummel, W.; Schuette, H.; Schmidt, E.; Kula, M.R.
Neue Möglichkeiten zur enzymatischen Herstellung von L-Phenylalanin
GBF Monogr. Ser. Volume date 1988
11
207-210
1989
Rhodococcus sp.
-
Manually annotated by BRENDA team
Cooper, A.J.L.; Leung, L.K.H.; Asano, Y.
Enzymatic cycling assay for phenylpyruvate
Anal. Biochem.
183
210-214
1989
Bacillus badius, Sporosarcina ureae
Manually annotated by BRENDA team
Asano, Y.; Nakazawa, A.
High yield synthesis of L-amino acids by phenylalanine dehydrogenase from Sporosarcina urea
Agric. Biol. Chem.
51
2035-2036
1987
Sporosarcina ureae
-
Manually annotated by BRENDA team
Kataoka, K.; Takada, H.; Tanizawa, K.; Yoshimura, T.; Esaki, N.; Ohshima, T.; Soda, K.
Construction and characterization of chimeric enzyme consisting of an amino-terminal domain of phenylalanine dehydrogenase and a carboxy-terminal domain of leucine dehydrogenase
J. Biochem.
116
931-936
1994
Thermoactinomyces intermedius
Manually annotated by BRENDA team
Kataoka, K.; Tanizawa, K.; Fukui, T.; Ueno, H.; Yoshimura, T.; Esaki, N.; Soda, K.
Identification of active site lysyl residues of phenylalanine dehydrogenase by chemical modification with methyl acetyl phosphate combined with site-directed mutagenesis
J. Biochem.
116
1370-1376
1994
Thermoactinomyces intermedius
Manually annotated by BRENDA team
Kataoka, K.; Takada, H.; Yoshimura, T.; Furuyoshi, S.; Esaki, N.; Ohshima, T.; Soda, K.
Site-directed mutagenesis of a hexapeptide segment involved in substrate recognition of phenylalanine dehydrogenase from Thermoactinomyces intermedius
J. Biochem.
114
69-75
1993
Thermoactinomyces intermedius
Manually annotated by BRENDA team
Asano, Y.; Tanetani, M.
Thermostable phenylalanine dehydrogenase from a mesophilic Microbacterium sp. strain DM 86-1
Arch. Microbiol.
169
220-224
1998
Microbacterium sp., Microbacterium sp. DM 86-1
Manually annotated by BRENDA team
Brunhuber, N.M.W.; Thoden, J.B.; Blanchard, J.S.; Vanhooke, J.L.
Rhodococcus L-Phenylalanine Dehydrogenase: Kinetics, Mechanism, and Structural Basis for Catalytic Specifity
Biochemistry
39
9174-9187
2000
Rhodococcus sp.
Manually annotated by BRENDA team
Vanhooke, J.L.; Thoden, J.B.; Brunhuber, N.M.W.; Blanchard, J.S.; Holden, H.M.
Phenylalanine dehydrogenase from Rhodococcus sp. M4: High-resolution x-ray analyses of inhibitory ternary complexes reveal key features in the oxidative deamination mechanism
Biochemistry
38
2326-2339
1999
Rhodococcus sp.
Manually annotated by BRENDA team
Hanson, R.L.; Howell, J.M.; LaPorte, T.L.; Donovan, M.J.; Cazzulino, D.L.; Zannella, V.; Montana, M.A.; Nanduri, V.B.; Schwarz, S.R.; Eiring, R.F.; Durand, S.C.; Wasylyk, J.M.; Parker, W.L.; Liu, M.S.; Okuniewicz, F.J.; Chen, B.C.; Harris, J.C.; Natalie,
Synthesis of allysine ethylene acetal using phenylalanine dehydrogenase from Thermoactinomyces intermedius
Enzyme Microb. Technol.
26
348-358
2000
Thermoactinomyces intermedius
Manually annotated by BRENDA team
Seah, S.Y.; Britton, K.L.; Baker, P.J.; Rice, D.W.; Asano, Y.; Engel, P.C.
Alteration in relative activities of phenylalanine dehydrogenase towards different substrates by site-directed mutagenesis
FEBS Lett.
370
93-96
1995
Lysinibacillus sphaericus
Manually annotated by BRENDA team
Pasquo, A.; Britton, K.L.; Baker, P.J.; Brearley, G.; Hinton, R.J.; Moir, A.J.G.; Stillman, T.J.; Rice, D.W.
Crystallization of NAD+-dependent phenylalanine dehydrogenase from Nocardia sp239
Acta Crystallogr. Sect. D
54
269-272
1998
Nocardia sp., Nocardia sp. 239
-
Manually annotated by BRENDA team
Seah, S.Y.; Britton, K.L.; Rice, D.W.; Asano, Y.; Engel, P.C.
Single amino acid substitution in Bacillus sphaericus phenylalanine dehydrogenase dramatically increases its discrimination between phenylalanine and tyrosine substrates
Biochemistry
41
11390-11397
2002
Bacillus badius, Lysinibacillus sphaericus
Manually annotated by BRENDA team
Seah, S.Y.; Britton, K.L.; Rice, D.W.; Asano, Y.; Engel, P.C.
Kinetic analysis of phenylalanine dehydrogenase mutants designed for aliphatic amino acid dehydrogenase activity with guidance from homology-based modelling
Eur. J. Biochem.
270
4628-4634
2003
Lysinibacillus sphaericus
Manually annotated by BRENDA team
Busca, P.; Paradisi, F.; Moynihan, E.; Maguire, A.R.; Engel, P.C.
Enantioselective synthesis of non-natural amino acids using phenylalanine dehydrogenases modified by site-directed mutagenesis
Org. Biomol. Chem.
2
2684-2691
2004
Lysinibacillus sphaericus
Manually annotated by BRENDA team
Omidinia, E.; Samadi, A.; Taherkhani, H.; Khatami, S.; Moazami, N.; Pouraie, R.R.; Asano, Y.
Cloning and expression of Bacillus sphaericus phenylalanine dehydrogenase gene in Bacillus subtilis cells: purification and enzyme properties
World J. Microbiol. Biotechnol.
18
593-597
2002
Lysinibacillus sphaericus
-
Manually annotated by BRENDA team
Villalonga, R.; Tachibana, S.; Perez, Y.; Asano, Y.
Increased conformational and thermal stability properties for phenylalanine dehydrogenase by chemical glycosidation with end-group activated dextran
Biotechnol. Lett.
27
1311-1317
2005
Bacillus badius
Manually annotated by BRENDA team
Villalonga, R.; Fujii, A.; Shinohara, H.; Asano, Y.; Cao, R.; Tachibana, S.; Ortiz, P.
Supramolecular-mediated immobilization of L-phenylalanine dehydrogenase on cyclodextrin-coated Au electrodes for biosensor applications
Biotechnol. Lett.
30
26-32
2007
Bacillus badius
Manually annotated by BRENDA team
Faulkner, E.; Barrett, M.; Okor, S.; Kieran, P.; Casey, E.; Paradisi, F.; Engel, P.; Glennon, B.
Use of fed-batch cultivation for achieving high cell densities for the pilot-scale production of a recombinant protein (phenylalanine dehydrogenase) in Escherichia coli
Biotechnol. Prog.
22
889-897
2006
Lysinibacillus sphaericus
Manually annotated by BRENDA team
Cainelli, G.; Engel, P.C.; Galletti, P.; Giacomini, D.; Gualandi, A.; Paradisi, F.
Engineered phenylalanine dehydrogenase in organic solvents: homogeneous and biphasic enzymatic reactions
Org. Biomol. Chem.
3
4316-4320
2005
Lysinibacillus sphaericus
Manually annotated by BRENDA team
Hanson, R.L.; Goldberg, S.L.; Brzozowski, D.B.; Tully, T.P.; Cazzulino, D.; Parker, W.L.; Lyngberg, O.K.; Vu, T.C.; Wong, M.K.; Patel, R.N.
Preparation of an amino acid intermediate for the dipeptidyl peptidase IV inhibitor, saxagliptin, using a modified phenylalanine dehydrogenase
Adv. Synth. Catal.
349
1369-1378
2007
Thermoactinomyces intermedius
-
Manually annotated by BRENDA team
Villalonga, R.; Fujii, A.; Shinohara, H.; Asano, Y.; Cao, R.; Tachibana, S.; Ortiz, P.
Supramolecular-mediated immobilization of L-phenylalanine dehydrogenase on cyclodextrin-coated Au electrodes for biosensor applications
Biotechnol. Lett.
29
447-452
2007
Bacillus badius
Manually annotated by BRENDA team
Chen, S.; Engel, P.C.
An engineered mutant, L307V of phenylalanine dehydrogenase from Bacillus sphaericus: high activity and stability in organic-aqueous solvent mixtures and utility for synthesis of non-natural L-amino acids
Enzyme Microb. Technol.
40
1407-1411
2007
Lysinibacillus sphaericus
-
Manually annotated by BRENDA team
Villalonga, R.; Tachibana, S.; Cao, R.; Matos, M.; Asano, Y.
Glycosidation of phenylalanine dehydrogenase with O-carboxymethyl-poly-beta-cyclodextrin
Enzyme Microb. Technol.
40
471-475
2007
Bacillus badius
-
Manually annotated by BRENDA team
Shahbaz Mohammadi, H.; Omidinia, E.; Taherkhani, H.
Rapid one-step separation and purification of recombinant phenylalanine dehydrogenase in aqueous two-phase systems
Iran. Biomed. J.
12
115-122
2008
Lysinibacillus sphaericus
Manually annotated by BRENDA team
Paradisi, F.; Collins, S.; Maguire, A.R.; Engel, P.C.
Phenylalanine dehydrogenase mutants: Efficient biocatalysts for synthesis of non-natural phenylalanine derivatives
J. Biotechnol.
128
408-411
2007
Lysinibacillus sphaericus
Manually annotated by BRENDA team
Mohamadi, H.S.; Omidinia, E.
Purification of recombinant phenylalanine dehydrogenase by partitioning in aqueous two-phase systems
J. Chromatogr. B
854
273-278
2007
Bacillus badius
Manually annotated by BRENDA team
Villalonga, R.; Fujii, A.; Shinohara, H.; Tachibana, S.; Asano, Y.
Covalent immobilization of phenylalanine dehydrogenase on cellulose membrane for biosensor construction
Sens. Actuators B Chem.
B129
195-199
2008
Bacillus badius
-
Manually annotated by BRENDA team
Omidinia, E.; Shahbaz Mohamadi, H.; Dinarvand, R.; Taherkhani, H.A.
Investigation of chromatography and polymer/salt aqueous two-phase processes for downstream processing development of recombinant phenylalanine dehydrogenase
Bioprocess Biosyst. Eng.
33
317-329
2010
Bacillus badius
Manually annotated by BRENDA team
Chen, S.; Engel, P.C.
Efficient screening for new amino acid dehydrogenase activity: directed evolution of Bacillus sphaericus phenylalanine dehydrogenase towards activity with an unsaturated non-natural amino acid
J. Biotechnol.
142
127-134
2009
Lysinibacillus sphaericus
Manually annotated by BRENDA team
Tarhan, L.; Ayar-Kayali, H.
Immobilization of phenylalanine dehydrogenase and its application in flow-injection analysis system for determination of plasma phenylalanine
Appl. Biochem. Biotechnol.
163
258-267
2011
Sporosarcina ureae
Manually annotated by BRENDA team
Ahmad, A.; Low, E.; Shukor, S.
Immobilization of phenylalanine dehydrogenase onto Eupergit CM for the synthesis of (S)-2-amino-4-phenylbutyric acid
J. Mol. Catal. B
88
26-31
2013
Rhodococcus sp., Rhodococcus sp. 4
-
Manually annotated by BRENDA team
Nezafat, N.; Omidinia, E.; Zadeh, S.; Keshel, S.; Zandian, S.
Potential role of promoter in production level of Bacillus sphaericus phenylalanine dehydrogenase in Escherchia coli expression system
J. Pure Appl. Microbiol.
7
213-219
2013
Lysinibacillus sphaericus
-
Manually annotated by BRENDA team
Ye, L.; Toh, H.; Yang, Y.; Adams, J.; Snajdrova, R.; Li, Z.
Engineering of amine dehydrogenase for asymmetric reductive amination of ketone by evolving Rhodococcus phenylalanine dehydrogenase
ACS Catal.
5
1119-1122
2015
Rhodococcus sp. (Q59771)
-
Manually annotated by BRENDA team
Zhang, J.; Zhu, T.; Wu, X.; Chen, Y.
Enhancement of biocatalytic efficiency by increasing substrate loading enzymatic preparation of L-homophenylalanine
Appl. Microbiol. Biotechnol.
97
8487-8494
2013
Thermoactinomyces intermedius (P22823)
Manually annotated by BRENDA team
Gubica, T.; Palka, K.; Szeleszczuk, A.; Kanska, M.
Enhanced enzymatic activity of phenylalanine dehydrogenase caused by cyclodextrins
J. Mol. Catal. B
118
89-94
2015
Sporosarcina sp.
-
Manually annotated by BRENDA team
Jiang, W.; Sun, D.; Ren, H.; Xu, C.; Wang, Y.; Zhang, Y.; Wang, S.; Fang, B.
Isolation, purification and characterization of a salt-active and organic-solvent-thermostable phenylalanine dehydrogenase from Bacillus nanhaiensis DSF-15A2
J. Mol. Catal. B
133
12-19
2016
Fictibacillus nanhaiensis (A0A1B1PGG4), Fictibacillus nanhaiensis DSF-15A2 (A0A1B1PGG4)
-
Manually annotated by BRENDA team