1.14.13.163: 6-hydroxy-3-succinoylpyridine 3-monooxygenase
This is an abbreviated version!
For detailed information about 6-hydroxy-3-succinoylpyridine 3-monooxygenase, go to the full flat file.
Word Map on EC 1.14.13.163
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1.14.13.163
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pyrrolidine
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putida
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2,5-dihydroxypyridine
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tumefaciens
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agrobacterium
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arthrobacter
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fad-binding
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ochrobactrum
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wastes
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synthesis
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nicotine-degrading
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flavoprotein
- 1.14.13.163
- pyrrolidine
- putida
- 2,5-dihydroxypyridine
- tumefaciens
-
agrobacterium
- arthrobacter
-
fad-binding
-
ochrobactrum
- wastes
- synthesis
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nicotine-degrading
- flavoprotein
Reaction
+ 2 NADH + 2 H+ + = + + 2 NAD+ +
Synonyms
6-hydroxy-3-succinoyl-pyridine 3-monooxygenase, 6-hydroxy-3-succinoylpyridine hydroxylase, AWN88_01205, Hsh, HSP 3-monooxygenase, HSP hydroxylase, HSP monooxygenase, hspA, hspB, HSPH, HSPHZZ, NADH-dependent HSP hydroxylase, NIC, nicotine hydroxylase, VppD, W7K_00670
ECTree
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Application
Application on EC 1.14.13.163 - 6-hydroxy-3-succinoylpyridine 3-monooxygenase
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synthesis
development of an efficient process to transform HSP into 2,5-dihydroxypyridine (2,5-DHP) with heterologously expressed HSP hydroxylase and NADH-regenerating system, because 2,5-DHP, the product of the reaction catalyzed by HSP hydroxylase, is a valuable precursor for the chemical synthesis of 5-aminolevulinic acid, which is applied as a plant growth hormone, a herbicide and in cancer therapy
synthesis
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enzyme HSPHZZ can be used for the enzymatic production of 2,5-dihydroxypyridine in biotechnology applications. 85.3 mg/l 2,5-dihydroxypyridine is produced in 40 min with a conversion of 74.9% at 30°C, pH 8.5, 1.0 mM substrate concentration, and 0.001 mM enzyme concentration
synthesis
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production of 2,5-dihydroxypyridine from 6-hydroxy-3-succinoylpyridine in the presence of NADH and FAD using nicotine hydroxylase covalently immobilized on Immobead 150. At a protein loading of 15 mg/g, ImmHSPHZZ converts 93.6% of 6-hydroxy-3-succinoylpyridine to 2,5-dihydroxypyridine in 6 h. The optimal concentrations of ImmHSPHZZ and substrate are 30 mg/l and 0.75 mM, respectively. Under optimal conditions, 94.5 mg/l of 2,5-dihydroxypyridine is produced after 30 min with 85.4% conversion
synthesis
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development of an efficient process to transform HSP into 2,5-dihydroxypyridine (2,5-DHP) with heterologously expressed HSP hydroxylase and NADH-regenerating system, because 2,5-DHP, the product of the reaction catalyzed by HSP hydroxylase, is a valuable precursor for the chemical synthesis of 5-aminolevulinic acid, which is applied as a plant growth hormone, a herbicide and in cancer therapy
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