1.14.13.163: 6-hydroxy-3-succinoylpyridine 3-monooxygenase

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For detailed information about 6-hydroxy-3-succinoylpyridine 3-monooxygenase, go to the full flat file.

Reaction

Synonyms

6-hydroxy-3-succinoyl-pyridine 3-monooxygenase, 6-hydroxy-3-succinoylpyridine hydroxylase, AWN88_01205, Hsh, HSP 3-monooxygenase, HSP hydroxylase, HSP monooxygenase, hspA, hspB, HSPH, HSPHZZ, NADH-dependent HSP hydroxylase, NIC, nicotine hydroxylase, VppD, W7K_00670

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.163 6-hydroxy-3-succinoylpyridine 3-monooxygenase

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Results	in table
1	Activating Compound
90	AA Sequence
4	Application
5	Cloned(Commentary)
15	Cofactor
2	Expression
20	General Information
23	Inhibitors
2	kcat/KM [mM/s]
11	KM Value [mM]
3	Metals/Ions
4	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
23	Organism
5	Pathway
2	PDB ID
7	pH Optimum
1	pH Range
2	pH Stability
2	Protein Variants
5	Purification (Commentary)
3	Reaction
17	Reference
1	Source Tissue
5	Specific Activity [micromol/min/mg]
3	Storage Stability
24	Substrates and Products (Substrate)
7	Subunits
40	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
1	Temperature Range [°C]
3	Temperature Stability [°C]
7	Turnover Number [1/s]

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Cloned on EC 1.14.13.163 - 6-hydroxy-3-succinoylpyridine 3-monooxygenase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

DNA and amino acid sequence determination and analysis, sequence comparisons, RT-PCR expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)

Agrobacterium tumefaciens

W8E0Y0

746248

expression in Escherichia coli

Pseudomonas putida

B1N1A2

690527

gene hspB, phylogenetic analysis and tree, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)

Pseudomonas putida

-

745310

gene vppD cloned from a 97-kbp DNA fragment containing six nicotine degradation-related genes which is obtained by gap closing from the genome sequence of strain SJY1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)

Ochrobactrum sp.

A0A075XAG5

741691

recombinant expression of His-tagged enzyme in Escherichia coli strain B21-Codon Plus(DE3)-RIL

Pseudomonas sp. ZZ-5

-

744640