1.1.99.31: (S)-mandelate dehydrogenase

This is an abbreviated version!
For detailed information about (S)-mandelate dehydrogenase, go to the full flat file.

Word Map on EC 1.1.99.31

Reaction

(S)-Mandelate
+
acceptor
=
phenylglyoxylate
+
reduced acceptor

Synonyms

(S)-mandelate dehydrogenase, L-mandelate dehydrogenase, L-MDH, MDH, SManDH, SMDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.99 With unknown physiological acceptors
                1.1.99.31 (S)-mandelate dehydrogenase

Turnover Number

Turnover Number on EC 1.1.99.31 - (S)-mandelate dehydrogenase

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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.03
(R,S)-p-chloromandelate
-
mutant enzyme H274G, in presence of 20 mM imidazole
201 - 270
(S)-3-phenyllactate
0.04 - 402
(S)-Mandelate
0.5
2-Hydroxy-3-butenoate
-
pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
3.9 - 14.8
2-Hydroxy-3-butynoate
0.1 - 0.37
2-Hydroxybutyrate
0.19 - 0.48
2-hydroxyhexanoate
0.48 - 1.28
2-hydroxyisocaproate
0.03
2-Hydroxyisovalerate
-
pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
0.26 - 1
2-Hydroxyoctanoate
0.27 - 0.5
2-hydroxyvalerate
313
2-oxo-2-phenylacetate
-
-
108
3-hydroxy-DL-mandelate
-
pH 7.5, 25°C, cosubstrate: ferricyanide
0.27 - 3.9
3-indolelactate
68
3-methoxy-DL-mandelate
-
pH 7.5, 25°C, cosubstrate: ferricyanide
0.15 - 0.87
3-Phenyllactate
108
4-bromo-DL-mandelate
-
pH 7.5, 25°C, cosubstrate: ferricyanide
68 - 116
4-chloro-DL-mandelate
9.3 - 334
4-chloromandelate
98
4-fluoro-DL-mandelate
-
pH 7.5, 25°C, cosubstrate: ferricyanide
146
4-hydroxy-DL-mandelate
-
pH 7.5, 25°C, cosubstrate: ferricyanide
106
4-methoxy-DL-mandelate
-
pH 7.5, 25°C, cosubstrate: ferricyanide
80
4-methyl-DL-mandelate
-
pH 7.5, 25°C, cosubstrate: ferricyanide
225
cytochrome c
-
pH 7.5, 25°C
94
D,L-Mandelate
-
pH 7.5, 25°C, cosubstrate: ferricyanid
155 - 316
DL-[2-1H]-mandelate
88 - 99
DL-[2-2H]-mandelate
0.3 - 204
ethyl-(S)-mandelate
550
ferricyanide
-
pH 7.5, 25°C
1.2 - 122
indoleglycolate
114
L-Mandelate
-
pH 7.5, 25°C, cosubstrate: ferricyanide
2.3 - 350
Mandelate
2.3 - 151
methyl-(S)-mandelate
0.03 - 3.4
O2