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Literature summary for 1.1.99.31 extracted from

  • Illias, R.M.; Sinclair, R.; Robertson, D.; Neu, A.; Chapman, S.K.; Reid, G.A.
    L-Mandelate dehydrogenase from Rhodotorula graminis: cloning, sequencing and kinetic characterization of the recombinant enzyme and its independently expressed flavin domain (1998), Biochem. J., 333, 107-115.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhodotorula graminis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
DL-[2-1H]-mandelate pH 7.5, 25°C, cosubstrate ferricyanide, LMDH holoenzyme Rhodotorula graminis
0.53
-
DL-[2-2H]-mandelate pH 7.5, 25°C, cosubstrate ferricyanide, LMDH holoenzyme Rhodotorula graminis
0.74
-
DL-[2-1H]-mandelate pH 7.5, 25°C, cosubstrate ferricyanide, L-MDH flavin domain Rhodotorula graminis
0.78
-
DL-[2-1H]-mandelate pH 7.5, 25°C, cosubstrate cytochrome c, LMDH holoenzyme Rhodotorula graminis
0.79
-
DL-[2-2H]-mandelate pH 7.5, 25°C, cosubstrate ferricyanide, L-MDH flavin domain Rhodotorula graminis
1.33
-
DL-[2-2H]-mandelate pH 7.5, 25°C, cosubstrate cytochrome, LMDH holoenzyme Rhodotorula graminis

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Rhodotorula graminis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Rhodotorula graminis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-mandelate + 2 ferricyanide
-
Rhodotorula graminis 2-oxo-2-phenylacetate + 2 ferrocyanide + 2 H+
-
?
(S)-mandelate + cytochrome c
-
Rhodotorula graminis 2-oxo-2-phenylacetate + ?
-
?
DL-mandelate + ferricyanide
-
Rhodotorula graminis 2-oxo-2-phenylacetate + ferrocyanide + H2O
-
?

Synonyms

Synonyms Comment Organism
L-MDH
-
Rhodotorula graminis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
88
-
DL-[2-2H]-mandelate pH 7.5, 25°C, cosubstrate ferricyanide, LMDH holoenzyme Rhodotorula graminis
92
-
DL-[2-2H]-mandelate pH 7.5, 25°C, cosubstrate cytochrome, LMDH holoenzyme Rhodotorula graminis
99
-
DL-[2-2H]-mandelate pH 7.5, 25°C, cosubstrate ferricyanide, L-MDH flavin domain Rhodotorula graminis
155
-
DL-[2-1H]-mandelate pH 7.5, 25°C, cosubstrate cytochrome c, LMDH holoenzyme Rhodotorula graminis
225
-
cytochrome c pH 7.5, 25°C Rhodotorula graminis
300
-
DL-[2-1H]-mandelate pH 7.5, 25°C, cosubstrate ferricyanide, LMDH holoenzyme Rhodotorula graminis
316
-
DL-[2-1H]-mandelate pH 7.5, 25°C, cosubstrate ferricyanide, L-MDH flavin domain Rhodotorula graminis
550
-
ferricyanide pH 7.5, 25°C Rhodotorula graminis