Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rhodotorula graminis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.47 | - |
DL-[2-1H]-mandelate | pH 7.5, 25°C, cosubstrate ferricyanide, LMDH holoenzyme | Rhodotorula graminis | |
0.53 | - |
DL-[2-2H]-mandelate | pH 7.5, 25°C, cosubstrate ferricyanide, LMDH holoenzyme | Rhodotorula graminis | |
0.74 | - |
DL-[2-1H]-mandelate | pH 7.5, 25°C, cosubstrate ferricyanide, L-MDH flavin domain | Rhodotorula graminis | |
0.78 | - |
DL-[2-1H]-mandelate | pH 7.5, 25°C, cosubstrate cytochrome c, LMDH holoenzyme | Rhodotorula graminis | |
0.79 | - |
DL-[2-2H]-mandelate | pH 7.5, 25°C, cosubstrate ferricyanide, L-MDH flavin domain | Rhodotorula graminis | |
1.33 | - |
DL-[2-2H]-mandelate | pH 7.5, 25°C, cosubstrate cytochrome, LMDH holoenzyme | Rhodotorula graminis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula graminis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Rhodotorula graminis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-mandelate + 2 ferricyanide | - |
Rhodotorula graminis | 2-oxo-2-phenylacetate + 2 ferrocyanide + 2 H+ | - |
? | |
(S)-mandelate + cytochrome c | - |
Rhodotorula graminis | 2-oxo-2-phenylacetate + ? | - |
? | |
DL-mandelate + ferricyanide | - |
Rhodotorula graminis | 2-oxo-2-phenylacetate + ferrocyanide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-MDH | - |
Rhodotorula graminis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
88 | - |
DL-[2-2H]-mandelate | pH 7.5, 25°C, cosubstrate ferricyanide, LMDH holoenzyme | Rhodotorula graminis | |
92 | - |
DL-[2-2H]-mandelate | pH 7.5, 25°C, cosubstrate cytochrome, LMDH holoenzyme | Rhodotorula graminis | |
99 | - |
DL-[2-2H]-mandelate | pH 7.5, 25°C, cosubstrate ferricyanide, L-MDH flavin domain | Rhodotorula graminis | |
155 | - |
DL-[2-1H]-mandelate | pH 7.5, 25°C, cosubstrate cytochrome c, LMDH holoenzyme | Rhodotorula graminis | |
225 | - |
cytochrome c | pH 7.5, 25°C | Rhodotorula graminis | |
300 | - |
DL-[2-1H]-mandelate | pH 7.5, 25°C, cosubstrate ferricyanide, LMDH holoenzyme | Rhodotorula graminis | |
316 | - |
DL-[2-1H]-mandelate | pH 7.5, 25°C, cosubstrate ferricyanide, L-MDH flavin domain | Rhodotorula graminis | |
550 | - |
ferricyanide | pH 7.5, 25°C | Rhodotorula graminis |