Protein Variants | Comment | Organism |
---|---|---|
additional information | MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach. This mutant is very similar to the wild-type membrane-bound enzyme in its spectroscopic properties, substrate specificity, catalytic activity, kinetic mechanism, and lack of reactivity toeards oxygen. It should prove to be a highly useful model for structural studies of MDH | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.085 | - |
(S)-Mandelate | pH 7.5, 20°C, cosubstrate: 2,6-dichloroindophenol, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
0.14 | - |
Mandelate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
0.18 | - |
(S)-Mandelate | pH 7.5, 20°C, cosubstrate: 2,6-dichloroindophenol, wild-type enzyme | Pseudomonas putida | |
0.24 | - |
indoleglycolate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
0.29 | - |
2-Hydroxyoctanoate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
0.49 | - |
2-hydroxyisocaproate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
0.64 | - |
3-indolelactate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
0.89 | - |
2-hydroxyhexanoate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
1.3 | - |
3-Phenyllactate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
2.6 | - |
2-Hydroxyisovalerate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
3.2 | - |
2-hydroxyvalerate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
10.3 | - |
2-Hydroxy-3-butenoate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
13.2 | - |
2-Hydroxybutyrate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
17.2 | - |
2-Hydroxy-3-butynoate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | associated | Pseudomonas putida | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,S)-2-hydroxy-3-butenoic acid + acceptor | - |
Pseudomonas putida | 2-oxo-3-butenoic acid + reduced acceptor | - |
? | |
(R,S)-2-hydroxyisovalerate + acceptor | - |
Pseudomonas putida | 2-oxo-isovalerate + reduced acceptor | - |
? | |
(S)-mandelate + acceptor | - |
Pseudomonas putida | 2-oxo-2-phenylacetate + reduced acceptor | - |
? | |
2-hydroxy-3-butynoate + acceptor | - |
Pseudomonas putida | 2-oxo-3-butynoate + reduced acceptor | - |
? | |
2-hydroxybutyrate + acceptor | - |
Pseudomonas putida | 2-oxobutyrate + reduced acceptor | - |
? | |
2-hydroxyhexanoate + acceptor | - |
Pseudomonas putida | 2-oxohexanoate + reduced acceptor | - |
? | |
2-hydroxyisocaproate + acceptor | - |
Pseudomonas putida | 2-oxoisocaproate + reduced acceptor | - |
? | |
2-hydroxyoctanoate + acceptor | - |
Pseudomonas putida | 2-oxooctanoate + reduced acceptor | - |
? | |
2-hydroxyvalerate + acceptor | - |
Pseudomonas putida | 2-oxovalerate + reduced acceptor | - |
? | |
3-indolelactate + acceptor | - |
Pseudomonas putida | 3-(1H-indol-2-yl)-2-oxopropanoate + reduced acceptor | - |
? | |
3-phenyllactate + acceptor | - |
Pseudomonas putida | 2-oxo-3-phenylpropanoate + reduced acceptor | - |
? | |
4-chloromandelate + acceptor | - |
Pseudomonas putida | 2-oxo-2-(4-chlorophenyl)acetate + reduced acceptor | - |
? | |
indoleglycolate + acceptor | - |
Pseudomonas putida | indol-2-yl(oxo)acetate + reduced acceptor | - |
? | |
mandelate + acceptor | - |
Pseudomonas putida | 2-oxo-2-phenylacetate + reduced acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MDH | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
2-Hydroxyisovalerate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
0.1 | - |
2-Hydroxybutyrate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
0.48 | - |
2-hydroxyhexanoate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
0.5 | - |
2-hydroxyvalerate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
0.5 | - |
2-Hydroxy-3-butenoate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
0.87 | - |
3-Phenyllactate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
0.96 | - |
2-Hydroxyoctanoate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
1.28 | - |
2-hydroxyisocaproate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
3.13 | - |
3-indolelactate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
3.85 | - |
3-indolelactate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
6.6 | - |
2-Hydroxy-3-butynoate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
38.5 | - |
indoleglycolate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
195 | - |
Mandelate | pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
205 | - |
(S)-Mandelate | pH 7.5, 20°C, cosubstrate: 2,6-dichloroindophenol, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach | Pseudomonas putida | |
290 | - |
(S)-Mandelate | pH 7.5, 20°C, cosubstrate: 2,6-dichloroindophenol, wild-type enzyme | Pseudomonas putida |