1.1.1.201: 7beta-hydroxysteroid dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about 7beta-hydroxysteroid dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.1.1.201

Reaction

a 7beta-hydroxysteroid
+
NADP+
=
a 7-oxosteroid
+
NADPH
+
H+

Synonyms

7beta-HSD, 7beta-HSDH, 7beta-hydroxysteroid dehydrogenase, More, NADP-dependent 7beta-hydroxysteroid dehydrogenase, RUMGNA_02585

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.201 7beta-hydroxysteroid dehydrogenase (NADP+)

Crystallization

Crystallization on EC 1.1.1.201 - 7beta-hydroxysteroid dehydrogenase (NADP+)

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified His-tagged enzyme complexed with NADP+, mixing of 10-15 mg/ml protein in 20% v/v glycerol, 20 mM potassium phosphate, pH 7.0, and 5 mM NADP+, with1 M sodium formate, 100 mM sodium acetate, pH 4.6, at room temperature, 3-4days, X-ray diffraction structure determination and analysis at 1.6 A resolution, modelling, molecular replacement using the the apoform structure of 7alpha-HSDH from Escherichia coli as search model, PDB ID 1AHH