1.1.1.201: 7beta-hydroxysteroid dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about 7beta-hydroxysteroid dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.1.1.201

Reaction

a 7beta-hydroxysteroid
+
NADP+
=
a 7-oxosteroid
+
NADPH
+
H+

Synonyms

7beta-HSD, 7beta-HSDH, 7beta-hydroxysteroid dehydrogenase, More, NADP-dependent 7beta-hydroxysteroid dehydrogenase, RUMGNA_02585

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.201 7beta-hydroxysteroid dehydrogenase (NADP+)

Purification

Purification on EC 1.1.1.201 - 7beta-hydroxysteroid dehydrogenase (NADP+)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme from liver microsomes 300fold by ion exchange, hydroxylapatite, 2',5'-ADP affinity
-
recombinant His6-tagged enzyme from Escherichia coli strain JM109 by nickel affinity chromatography, dialysis, and ultrafiltration
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration
to homogeneity, chromatography techniques