Information on EC 2.5.1.2 - thiamine pyridinylase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.5.1.2
-
RECOMMENDED NAME
GeneOntology No.
thiamine pyridinylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
thiamine + pyridine = 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyridinium + 4-methyl-5-(2-hydroxyethyl)thiazole
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pyrimidyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Thiamine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
thiamine:base 2-methyl-4-aminopyrimidine-5-methenyltransferase
Various bases and thiol compounds can act instead of pyridine.
CAS REGISTRY NUMBER
COMMENTARY hide
9030-35-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Anadara inflata
-
-
-
Manually annotated by BRENDA team
eel
-
-
Manually annotated by BRENDA team
Athyrium nipponicum
-
-
-
Manually annotated by BRENDA team
Auxis hira
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
crucian carp
-
-
Manually annotated by BRENDA team
Celosia crista
cockscomb
-
-
Manually annotated by BRENDA team
Charybdis 6-dentata
-
-
-
Manually annotated by BRENDA team
rock fern
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Clava kochi
-
-
-
Manually annotated by BRENDA team
var. Typican
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
fresh-water mussel
-
-
Manually annotated by BRENDA team
Dicranopteris sp.
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
horsetail
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
very low activity
-
-
Manually annotated by BRENDA team
Gnathopogon mayedae
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Haliotis japonica
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Mactra sulcataria
-
-
-
Manually annotated by BRENDA team
nardoo
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
clam
-
-
Manually annotated by BRENDA team
grey mullet
-
-
Manually annotated by BRENDA team
no activity in Astrocanger myriaster
-
-
-
Manually annotated by BRENDA team
no activity in Cambaroides japonicus
-
-
-
Manually annotated by BRENDA team
no activity in Camposcia retusa
-
-
-
Manually annotated by BRENDA team
no activity in Penaeopsis acclivis
-
-
-
Manually annotated by BRENDA team
no activity in Plecoglossus altivelis
-
-
-
Manually annotated by BRENDA team
no activity in Squilla oratoria
-
-
-
Manually annotated by BRENDA team
osmund royal
-
-
Manually annotated by BRENDA team
Ostrea laperousei
oyster
-
-
Manually annotated by BRENDA team
strain YUSM I00I
-
-
Manually annotated by BRENDA team
spiny lobster
-
-
Manually annotated by BRENDA team
Paphia philippinarum
-
-
-
Manually annotated by BRENDA team
var. Japonicum
-
-
Manually annotated by BRENDA team
Polystichum falcatum
-
-
-
Manually annotated by BRENDA team
Polystichum fortunei
-
-
-
Manually annotated by BRENDA team
var. Japonicum, bracken fern
-
-
Manually annotated by BRENDA team
bracken fern
-
-
Manually annotated by BRENDA team
baltic salmon
-
-
Manually annotated by BRENDA team
catfish
-
-
Manually annotated by BRENDA team
Solen gouldi
razor shell
-
-
Manually annotated by BRENDA team
the thiaminase activity in Clupea harengus membras is about 10fold higher than that in Sprattus sprattus
-
-
Manually annotated by BRENDA team
Thiara libertina
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
freshwater mussel
-
-
Manually annotated by BRENDA team
Viviparus malleatus
mud snail
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1'-methylthiamine + aniline
4-methyl-5-(2-hydroxyethyl)-thiazole + 4-amino-5-(anilinomethyl)-1,2-dimethylpyrimidin-1-ium
show the reaction diagram
-
22% of activity with thiamine
-
-
?
2'-ethylthiamine + aniline
4-methyl-5-(2-hydroxyethyl)-thiazole + 5-(anilinomethyl)-2-ethylpyrimidin-4-amine
show the reaction diagram
-
63% of activity with thiamine
-
-
?
2-(1-hydroxyethyl)thiamine + aniline
4-methyl-2-(1-hydroxyethyl)-5-(2-hydroxyethyl)-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
show the reaction diagram
-
92% of activity with thiamine
-
-
?
5-chloroethylthiamine + aniline
4-methyl-5-chloroethyl-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
show the reaction diagram
-
67% of activity with thiamine
-
-
?
5-nor-thiamine + aniline
4-methyl-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
show the reaction diagram
-
88% of activity with thiamine
-
-
?
O-(beta-D-galactosyl)thiamine + aniline
4-methyl-5-(2-hydroxyethyl-O-beta-D-galactosyl)-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
show the reaction diagram
-
63% of activity with thiamine
-
-
?
O-benzoylthiamine + aniline
2-(4-methyl-1,3-thiazol-5-yl)ethyl benzoate + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
show the reaction diagram
-
91% of activity with thiamine
-
-
?
O-S-diacetylthiamine + aniline
1-acetyl-4-methyl-5-(2-hydroxyethyl-O-acetyl)-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
show the reaction diagram
-
50% of activity with thiamine
-
-
?
O-succinylthiamine + aniline
4-methyl-5-(2-hydroxyethyl-O-succinyl)-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
show the reaction diagram
-
83% of activity with thiamine
-
-
?
pyrithiamine + aniline
2-(2-methylpyridin-3-yl)ethanol + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
show the reaction diagram
-
72% of activity with thiamine
-
-
?
tetrahydrothiamine + aniline
?
show the reaction diagram
-
84% of activity with thiamine
-
-
?
thiamine
?
show the reaction diagram
thiamine + 2-aminopyridine
?
show the reaction diagram
-
-
-
-
-
thiamine + 2-hydroxyaniline
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-hydroxyphenyl)amine
show the reaction diagram
-
-
-
-
?
thiamine + 2-mercaptobenzoic acid
?
show the reaction diagram
-
-
-
-
?
thiamine + 2-mercaptoethanol
?
show the reaction diagram
thiamine + 2-methylbenzeneamine
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-methylphenyl)amine
show the reaction diagram
-
i.e. o-toluidine
-
-
?
thiamine + 2-pyridinecarboxylic acid
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxypyridinium
show the reaction diagram
-
i.e. alpha-picolinic acid
-
-
?
thiamine + 3-aminopyridine
?
show the reaction diagram
thiamine + 3-hydroxyaniline
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-hydroxyphenyl)amine
show the reaction diagram
-
-
-
-
?
thiamine + 3-methylbenzeneamine
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-methylphenyl)amine
show the reaction diagram
-
i.e. m-toluidine
-
-
?
thiamine + 3-pyridinecarboxylic acid
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-3-carboxypyridinium
show the reaction diagram
-
i.e. beta-picolinic acid
-
-
?
thiamine + 4-aminopyridine
?
show the reaction diagram
thiamine + 4-hydroxyaniline
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-hydroxyphenyl)amine
show the reaction diagram
-
-
-
-
?
thiamine + 4-methylbenzeneamine
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-methylphenyl)amine
show the reaction diagram
-
i.e. p-toluidine
-
-
?
thiamine + 4-nitrothiophenolate
4-methyl-5-(2-hydroxyethyl)thiazole + 2-methyl-5[[(4-nitrophenyl)sulfanyl]methyl]pyrimidin-4-amine
show the reaction diagram
no activity with thiaminase II
-
-
?
thiamine + 4-nitrothiophenolate
?
show the reaction diagram
-
-
-
?
thiamine + 4-pyridinecarboxylic acid
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-carboxypyridinium
show the reaction diagram
-
i.e. gamma-picolinic acid
-
-
?
thiamine + 6-aminohexanoic acid
4-methyl-5-(2-hydroxyethyl)-thiazole + 6-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]hexanoic acid
show the reaction diagram
-
-
-
-
?
thiamine + adenine
?
show the reaction diagram
-
-
-
-
?
thiamine + alpha-picoline
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]alpha-picolinium
show the reaction diagram
-
-
-
-
?
thiamine + aniline
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
show the reaction diagram
thiamine + beta-picoline
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]beta-picolinium
show the reaction diagram
-
-
-
-
?
thiamine + cysteine
?
show the reaction diagram
-
-
-
-
?
thiamine + gamma-picoline
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]gamma-picolinium
show the reaction diagram
-
-
-
-
?
thiamine + hydroxyproline
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid
show the reaction diagram
thiamine + imidazole
4-methyl-5-(2-hydroxyethyl)-thiazole + 5-(1H-imidazol-1-yl-methyl)-2-methylpyrimidin-4-amine
show the reaction diagram
thiamine + L-cysteine
4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-ethylpyrimidin-5-yl)methyl]amino]-3-mercaptopropanoic acid
show the reaction diagram
-
50% of activity with aniline
-
-
?
thiamine + L-lysine
?
show the reaction diagram
-
-
-
-
?
thiamine + m-aminobenzoic acid
4-methyl-5-(2-hydroxyethyl)-thiazole + 3-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid
show the reaction diagram
-
-
-
-
?
thiamine + m-phenylene diamine
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-aminophenyl)amine
show the reaction diagram
-
-
-
-
?
thiamine + o-aminobenzoic acid
4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid
show the reaction diagram
-
-
-
-
?
thiamine + o-phenylene diamine
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-aminophenyl)amine
show the reaction diagram
-
-
-
-
?
thiamine + p-aminobenzoic acid
4-methyl-5-(2-hydroxyethyl)-thiazole + 4-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid
show the reaction diagram
-
-
-
-
?
thiamine + p-phenylene diamine
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-aminophenyl)amine
show the reaction diagram
-
-
-
-
?
thiamine + proline
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid
show the reaction diagram
thiamine + pyridine
1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyridinium + 4-methyl-5-(2-hydroxyethyl)thiazole
show the reaction diagram
thiamine + pyridine
heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
show the reaction diagram
thiamine + pyridoxine
?
show the reaction diagram
-
-
-
-
?
thiamine + quinoline
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]quinolinium
show the reaction diagram
thiamine + veratrylamine
bis-(4-amino-2-methyl)pyrimidinyl-3,4-dimethoxybenzylamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
show the reaction diagram
the reaction can occur without base substrate
-
?
thiamine disulfide + aniline
?
show the reaction diagram
-
26% of activity with thiamine
-
-
?
thiamine phosphate + aniline
2-(4-methyl-1,3-thiazol-5-yl)ethyl dihydrogenphosphate + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
show the reaction diagram
-
64% of activity with thiamine
-
-
?
thiothiamine + aniline
4-methyl-5-(2-hydroxyethyl)-2-thio-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
show the reaction diagram
-
12% of activity with thiamine
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
thiamine + adenine
?
show the reaction diagram
-
-
-
-
-
thiamine + hydroxyproline
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid
show the reaction diagram
thiamine + proline
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid
show the reaction diagram
thiamine + pyridine
1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyridinium + 4-methyl-5-(2-hydroxyethyl)thiazole
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
-
weak activation with thiamine and aniline as substrates
additional information
-
Ca2+, Mo+, Mn2+, Zn2+, Mg2+ have no effect on activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-Amino-5-(anilinomethyl)-6-chloro-2-methylpyrimidine
-
complete irreversible inhibition 2 times faster than 4-amino-6-chloro-2-methylpyrimidine
4-Amino-6-chloro-2-methylpyrimidine
4-aminopyrimidines
Ag+
-
100% inhibition at 1 mM
aniline
Aromatic amines
-
inhibited by or without effect
-
Cd2+
-
the degree of cadmium inhibition, when aniline is the cosubstrate, shows obvious differences between pI isozymes
Dimethialium
-
inactivation at 0.006 mM
Heterocyclic amines
-
-
-
Heteropyrithiamine
HgCl2
Mersalyl acid
p-chloromercuribenzoate
thiamine
thiamine diphosphate
-
inactivation at 0.006 mM
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
4,5-dimethylthiazole
-
reactivation at 10 mM
5-(2-chloroethyl)-4-methylthiazole
-
reactivation at 10 mM
5-(2-hydroxyethyl)-4-methylthiazole
-
reactivation at 10 mM
aniline
Aromatic amines
-
cysteine
-
complete reactivation at 3.3 mM but inhibition at 33 mM
dithiothreitol
Heterocyclic amines
-
Pyridine
-
at 25 mM restores activity inhibited by heteropyrimidine at 0.006 mM
sulfhydryl compounds
Thioglycollate
-
greater reactivation at 33 mM than at 3.3 mM
thiosulphate
-
greater reactivation at 33 mM than at 3.3 mM
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
12.5
2-aminopyridine
-
-
4.2
2-hydroxyaniline
-
-
2.9
3-aminopyridine
-
-
2.8
3-hydroxyaniline
-
-
15.4
4-aminopyridine
-
-
20
4-Hydroxyaniline
-
-
0.0364
4-nitrothiophenolate
; pH 7.2, temperature not specified in the publication
38
6-aminohexanoic acid
-
-
9.5
adenine
-
-
3.3
alpha-picoline
-
-
21.2
alpha-picolinic acid
-
-
1.4 - 5.1
aniline
0.5
beta-picoline
-
-
25
beta-picolinic acid
-
-
33
cysteine
-
-
0.9
gamma-Picoline
-
-
3.4
gamma-picolinic acid
-
-
400
hydroxyproline
-
-
125
imidazole
-
-
37
m-aminobenzoic acid
-
-
50
m-nitroaniline
-
-
1.7
m-phenylene diamine
-
-
2.4
m-Toluidine
-
-
32
methylamine
-
-
0.9 - 55
nicotinic acid
37
o-aminobenzoic acid
-
-
5
o-mercaptobenzoic acid
-
best cosubstrate
1.7
o-phenylene diamine
-
-
6.4
o-toluidine
-
-
200
p-Aminobenzoic acid
-
-
3.3
p-phenylene diamine
-
-
111
proline
-
-
0.7 - 5
Pyridine
3.3
Quinoline
-
-
77
sulfanilamide
-
-
50
Sulfanilic acid
-
-
0.003 - 3.5
thiamine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
297
4-nitrothiophenolate
Paenibacillus thiaminolyticus
P45741
; pH 7.2, temperature not specified in the publication
0.01 - 260
thiamine
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8380
4-nitrothiophenolate
Paenibacillus thiaminolyticus
P45741
pH 7.2, temperature not specified in the publication
11902
0.07 - 12400
thiamine
642
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.096
4-Amino-5-(anilinomethyl)-6-chloro-2-methylpyrimidine
-
-
7.7
4-Amino-6-chloro-2-methylpyrimidine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8
-
pI 7-9 isoenzym, thiamine + 2-mercaptoethanol
8.2
-
pI 5.7 isoenzyme, thiamine + 2-mercaptoethanol; pI 5.7 isoenzyme, thiamine + aniline
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 12
5.8 - 6.8
-
assay at 25ºC
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
in 50 mM sodium phosphate at pH 7
45
-
pI 5.7 isoenzyme, pI 7-9 isoenzym, thiamine + 2-mercaptoethanol
45 - 55
-
pI 5.7 isoenzyme, pI 7-9 isoenzym, thiamine + aniline
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37 - 65
-
enzyme is stable
50 - 60
-
maximal activity
60
-
more than 90% of activity remains after 20 min
65
-
activity abolished after 20 min
70
-
50% denaturation
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.7
-
the liver of Fisturalia petimba contains thiaminase I of at least three different pIs and the major fraction exhibits pI 5.7. The most evident difference among pI isozymes is the size of the active subfragments into which they are dissociated. pI 5.7 enzyme dissociates into subfragments of 25 kDa, while pI 7-9 enzymes dissociates into approximately 22000 Da. The reaction rate measured by pyridine as the second substrate is three times higher in pI 7-9 enzymes compared with pI 5.7 enzyme. The degree of cadmium inhibition, when aniline is the cosubstrate, shows obvious differences between pI isozymes
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Celosia crista
-
less activity than in leaves
Manually annotated by BRENDA team
Celosia crista
-
-
Manually annotated by BRENDA team
-
scarcely found
Manually annotated by BRENDA team
-
lush growth
Manually annotated by BRENDA team
-
scarcely found
Manually annotated by BRENDA team
-
very low activity
Manually annotated by BRENDA team
additional information
-
thiaminase activity of undigested Baltic herring (Clupea harengus membras) found in Baltic salmon (Salmo salar) stomachs is significantly higher than that of trawl-caught Baltic herring from the same sea area, suggesting that there may be a higher risk of predation for Baltic herring with high thiaminase activity, possibly linked to their health. Thiaminase activity of the gastrointestinal contents of Baltic salmon, feeding almost entirely on Baltic herring in the Gulf of Bothnia, is significantly higher than for Baltic salmon feeding on both Baltic herring and sprat in the Gulf of Bothnia. Therefore, Baltic herring may be the major source of thiaminase for Baltic salmon. A tank experiment demonstrates that thiaminase activity in Baltic herring may vary, even within very short time periods
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
enzyme activity is mostly found in lysosomal fraction determined by differential centrifugation, sucrose and percoll gradients
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44000
-
sedimentation and diffusion data
55000
-
gel filtration
93000
-
gel filtration
106000
-
pI 5.7 isoenzyme, gel filtration
107000
-
gel filtration
110000
-
gel filtration
115000
-
gel filtration
additional information
a comparison of thiamin-binding protein tbpA from Escherichia coli and thiaminase-I, reveals structural similarity between the two proteins, specially in domain 1, suggesting that the two proteins evolve from a common ancestor
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
additional information
-
the purified preparation of the pI 5.7 isoenzyme gives an active 25 kDa subfragment by SDS-PAGE, together with a 15 kDa non-active subfragment. The enzyme is, thus, inferred to contain active subfragments together with the 15 kDa non-active fragments. Amino acid sequencing of the 25 kDa active subfragment reveals, together with the fully processed N-terminal sequence, two N-terminal peptides with extra Pro-Ser and Gly-Pro-Ser attached to it
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
inactive C143S mutant with bound thiamine at 2.2 A resolution. Each monomer contains one thiamine molecule in a V-shaped cleft between the two domains. Tyrosine residues, Tyr46, Tyr48, Tyr80, Tyr252, Tyr269, and Tyr300, form an outer collar of the cleft with most of the active site residues located at the bottom of the cleft. The catalytic cysteine residue Cys143 is located on beta6
-
by vapour-diffusion method
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 12
5.8
-
0.1 M sodium phosphate buffer
636846, 636850
6.5
-
0.15 M citrate-phosphate buffer
636849
7
-
50 mM sodium phosphate buffer, 2 mM dithiothreitol, 2 mM EDTA
636853, 636858
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
addition of dithiothreitol, 2 mM, reduces the loss of activity
-
636853
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium precipitation and gel filtration
-
by ammonium precipitation, CM-cellulose column, DEAE-cellulose column, Cibacron blue Sepharose column, Sephacryl S-300 column, Superdex-200 and Superdex-75 column
-
by ammonium precipitation, DEAE column, Cibacron blue dye affinity column
-
by ammonium precipitation, Sephadex G-25 column, Sephadex G-100 column and DEAE-Sephadex column
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by DEAE-cellulose chromatrography, ultrogel AcA 34 column, Sephadex G-100 column and phosphocellulose column
-
HisTrap FF column chromatography
-
pI 5.7 isoenzyme
-
Tris-glycine polyacrylamide electrophoresis
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloned in Escherichia coli with Gly6 changed to Ala6 with no apparent effect
-
expressed in Escherichia coli BL21(DE3) cells
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C143S
-
inactive
D302N
-
about 20fold decrease in kcat/Km value
D94N
-
about 10000fold decrease in kcat/Km value
E271Q
-
about 3000fold decrease in kcat/Km value
Y46F
-
about 2fold decrease in kcat/Km value
Y80F
-
about 1000fold decrease in kcat/Km value
C113S
-
inactive mutant at the active nucleophilic site
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
medicine
nutrition