Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | the degree of cadmium inhibition, when aniline is the cosubstrate, shows obvious differences between pI isozymes | Fistularia petimba |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | weak activation with thiamine and aniline as substrates | Fistularia petimba |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
106000 | - |
pI 5.7 isoenzyme, gel filtration | Fistularia petimba |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiamine + pyridine | Fistularia petimba | - |
1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyridinium + 4-methyl-5-(2-hydroxyethyl)thiazole | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fistularia petimba | - |
- |
- |
Purification (Comment) | Organism |
---|---|
pI 5.7 isoenzyme | Fistularia petimba |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Fistularia petimba | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiamine + 2-mercaptoethanol | - |
Fistularia petimba | ? | - |
? | |
thiamine + 3-aminopyridine | - |
Fistularia petimba | ? | - |
? | |
thiamine + 4-aminopyridine | - |
Fistularia petimba | ? | - |
? | |
thiamine + aniline | - |
Fistularia petimba | 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
thiamine + cysteine | - |
Fistularia petimba | ? | - |
? | |
thiamine + L-lysine | - |
Fistularia petimba | ? | - |
? | |
thiamine + pyridine | - |
Fistularia petimba | 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyridinium + 4-methyl-5-(2-hydroxyethyl)thiazole | - |
? | |
thiamine + pyridine | the reaction rate measured by pyridine as the second substrate is three times higher in pI 7-9 enzymes compared with pI 5.7 enzyme | Fistularia petimba | 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyridinium + 4-methyl-5-(2-hydroxyethyl)thiazole | - |
? | |
thiamine + pyridoxine | - |
Fistularia petimba | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the purified preparation of the pI 5.7 isoenzyme gives an active 25 kDa subfragment by SDS-PAGE, together with a 15 kDa non-active subfragment. The enzyme is, thus, inferred to contain active subfragments together with the 15 kDa non-active fragments. Amino acid sequencing of the 25 kDa active subfragment reveals, together with the fully processed N-terminal sequence, two N-terminal peptides with extra Pro-Ser and Gly-Pro-Ser attached to it | Fistularia petimba |
Synonyms | Comment | Organism |
---|---|---|
thiaminase I | - |
Fistularia petimba |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
pI 5.7 isoenzyme, pI 7-9 isoenzym, thiamine + 2-mercaptoethanol | Fistularia petimba |
45 | 55 | pI 5.7 isoenzyme, pI 7-9 isoenzym, thiamine + aniline | Fistularia petimba |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
pI 7-9 isoenzym, thiamine + 2-mercaptoethanol | Fistularia petimba |
8.2 | - |
pI 5.7 isoenzyme, thiamine + 2-mercaptoethanol | Fistularia petimba |
8.2 | - |
pI 5.7 isoenzyme, thiamine + aniline | Fistularia petimba |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Fistularia petimba | the liver of Fisturalia petimba contains thiaminase I of at least three different pIs and the major fraction exhibits pI 5.7. The most evident difference among pI isozymes is the size of the active subfragments into which they are dissociated. pI 5.7 enzyme dissociates into subfragments of 25 kDa, while pI 7-9 enzymes dissociates into approximately 22000 Da. The reaction rate measured by pyridine as the second substrate is three times higher in pI 7-9 enzymes compared with pI 5.7 enzyme. The degree of cadmium inhibition, when aniline is the cosubstrate, shows obvious differences between pI isozymes | - |
5.7 |