Literature summary for 2.5.1.2 extracted from

Liu, S.; Monks, N.R.; Hanes, J.W.; Begley, T.P.; Yu, H.; Moscow, J.A.
Sensitivity of breast cancer cell lines to recombinant thiaminase I (2010), Cancer Chemother. Pharmacol., 66, 171-179.

Search for more literature for 2.5.1.2

Application

Application	Comment	Organism
medicine	thiamine transporter THTR2 downregulation in breast tumors may present a nutritional vulnerability that can be exploited by thiaminase I enzyme therapy	Paenibacillus thiaminolyticus

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Paenibacillus thiaminolyticus

Organism

Organism	UniProt	Comment	Textmining
Paenibacillus thiaminolyticus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap FF column chromatography	Paenibacillus thiaminolyticus

Synonyms

Synonyms	Comment	Organism
thiaminase I	-	Paenibacillus thiaminolyticus

General Information

General Information	Comment	Organism
physiological function	although thiaminase I exposure does not stimulate the energy-sensing signaling kinases AKT, AMPK and GSK-3beta in MCF-7, ZR75, HS-578T and T-47D cell lines, thiaminase I exposure does stimulate expression of the ER stress response protein GRP78. Thiaminase I is cytotoxic in breast cancer cell lines and triggers the unfolded protein response	Paenibacillus thiaminolyticus

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Release 2023.1 (January 2023)