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Literature summary for 2.5.1.2 extracted from
- The extracellular thiaminase I of Bacillus thiaminolyticus. I. Purification and physicochemical properties (1968), Biochemistry, 7, 736-744.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aniline | highly inhibitory above 2.4 mM | Paenibacillus thiaminolyticus |  |
| thiamine | slightly inhibitory above 0.035 mM | Paenibacillus thiaminolyticus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0087 | - |
thiamine | pH 5.8, 25°C, the enzyme does not follow strict Michaelis-Menten kinetics | Paenibacillus thiaminolyticus | |
| 2.9 | - |
aniline | pH 5.8, 25°C, the enzyme does not follow strict Michaelis-Menten kinetics | Paenibacillus thiaminolyticus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Paenibacillus thiaminolyticus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
sedimentation and diffusion data | Paenibacillus thiaminolyticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenibacillus thiaminolyticus | - |
M strain | - |
| Paenibacillus thiaminolyticus M | - |
M strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by ammonium precipitation, Sephadex G-25 column, Sephadex G-100 column and DEAE-Sephadex column | Paenibacillus thiaminolyticus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Paenibacillus thiaminolyticus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiamine + aniline | - |
Paenibacillus thiaminolyticus | 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
| thiamine + aniline | - |
Paenibacillus thiaminolyticus M | 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
in 0.1 M sodium phosphate buffer at pH 5.8 | Paenibacillus thiaminolyticus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
more than 90% of activity remains after 20 min | Paenibacillus thiaminolyticus |
| 65 | - |
activity abolished after 20 min | Paenibacillus thiaminolyticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 6.8 | assay at 25ºC | Paenibacillus thiaminolyticus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | - |
0.1 M sodium phosphate buffer | Paenibacillus thiaminolyticus |
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