Information on EC 2.4.1.69 - type 1 galactoside alpha-(1,2)-fucosyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.4.1.69
-
RECOMMENDED NAME
GeneOntology No.
type 1 galactoside alpha-(1,2)-fucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ABH and Lewis epitopes biosynthesis from type 1 precursor disaccharide
-
-
biosynthesis of Lewis epitopes (H. pylori)
-
-
Glycosphingolipid biosynthesis - globo and isoglobo series
-
-
Glycosphingolipid biosynthesis - lacto and neolacto series
-
-
lacto-series glycosphingolipids biosynthesis
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
GDP-beta-L-fucose:beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R alpha-(1,2)-L-fucosyltransferase (configuration-inverting)
Free lactose can act as acceptor. Normally acts on a glycoconjugate where R (see reaction) is a glycoprotein or glycolipid. The action on glycolipid was previously listed as EC 2.4.1.89.
CAS REGISTRY NUMBER
COMMENTARY hide
56093-23-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
-
TREMBL
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
fragment
UniProt
Manually annotated by BRENDA team
a FUT2-like gene sequence in opossum is found
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
-
in roots, isoforms FUT4 and FUT6 genes are responsible for arabinogalactan fucosylation
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
GDP-beta-L-fucose + D-Gal-beta-(1,2)-D-Glc-beta-O-methyl
GDP + L-Fuc-alpha-(1,2)-D-Gal-beta-(1,2)-D-Glc-beta-O-methyl
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + D-Gal-beta-(1,4)-D-Glc-beta-O-methyl
GDP + L-Fuc-alpha-(1,2)-D-Gal-beta-(1,4)-D-Glc-beta-O-methyl
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + D-Gal-beta-(1,6)-D-Glc-beta-O-methyl
GDP + L-Fuc-alpha-(1,2)-D-Gal-beta-(1,6)-D-Glc-beta-O-methyl
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + D-Gal-beta-(1->3)-D-Glcbeta-O-methyl
GDP + L-Fuc-alpha-(1->2)-D-Gal-beta-(1->3)-D-Glc-beta-O-methyl
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + D-galactose
?
show the reaction diagram
GDP-beta-L-fucose + D-lactose
GDP + 2'-fucosyl-D-lactose
show the reaction diagram
GDP-beta-L-fucose + D-lactulose
GDP + 2'-fucosyl-D-lactulose
show the reaction diagram
GDP-beta-L-fucose + Gal(beta1-3)GlcNAc
?
show the reaction diagram
28% activity compared to D-lactose
-
-
?
GDP-beta-L-fucose + Gal(beta1-4)GlcNAc
?
show the reaction diagram
85% activity compared to D-lactose
-
-
?
GDP-beta-L-fucose + Gal-beta-OR
GDP + L-Fucalpha(1-2)Galbeta-OR
show the reaction diagram
GDP-beta-L-fucose + galactose
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + Galbeta(1,3)GalNAcalpha-O-Me
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + Galbeta(1,3)GalNAcalpha-O-phenyl
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + Galbeta(1,4)Glcbeta-1-NAc
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + Galbeta(1,4)Glcbeta-N3
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + Galbeta(1,4)Glcbeta-O-phenyl
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + Galbeta(1,4)Glcbeta-S-phenyl
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + Galbeta(1,4)Man
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + Galbeta(1-3)GalNAc
GDP + Fucalpha(1-2)Galbeta(1-3)GalNAc
show the reaction diagram
-
-
-
-
ir
GDP-beta-L-fucose + Galbeta(1-3)GalNAc
GDP + L-Fucalpha(1-2)Galbeta(1-3)GalNAc
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + Galbeta(1-3)GalNAcalpha-O-p-nitrophenol
GDP + Fucalpha(1-2)Galbeta(1-3)GalNAcalpha-O-p-nitrophenol
show the reaction diagram
-
-
-
-
ir
GDP-beta-L-fucose + Galbeta(1-3)GalNAcalpha-OMe
GDP + Fucalpha(1-2)Galbeta(1-3)GalNAcalpha-OMe
show the reaction diagram
GDP-beta-L-fucose + Galbeta(1-3)GalNAcalpha-R
GDP + Fucalpha(1-2)Galbeta(1-3)GalNAcalpha-R
show the reaction diagram
-
-
-
-
ir
GDP-beta-L-fucose + Galbeta(1-3)GlcNAcbeta-O-p-nitrophenol
GDP + Fucalpha(1-2)Galbeta(1-3)GlcNAcbeta-O-p-nitrophenol
show the reaction diagram
-
activity is low
-
-
ir
GDP-beta-L-fucose + Galbeta(1-3)[Fucalpha(1-4)]GlcNAc
GDP + Fucalpha(1-2)Galbeta(1-3)[Fucalpha(1-4)]GlcNAc
show the reaction diagram
-
activity is low
-
-
ir
GDP-beta-L-fucose + Galbeta(1-3)[GlcNAcbeta(1-6)]GalNAcalpha-O-p-nitrophenol
GDP + Fucalpha(1-2)Galbeta(1-3)[GlcNAcbeta(1-6)]GalNAcalpha-O-p-nitrophenol
show the reaction diagram
-
-
-
-
ir
GDP-beta-L-fucose + Galbeta-4-nitrophenyl
?
show the reaction diagram
28% activity compared to D-lactose
-
-
?
GDP-beta-L-fucose + Galbeta-O-Me
GDP + L-Fucalpha(1-2)Galbeta-O-Me
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + Galbeta-p-nitrophenol
GDP + L-Fucalpha(1-2)Galbeta-p-nitrophenol
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + lactose
GDP + fucosyllactose
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + lactulose
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-fucose + asialofetuin
GDP + asialofetuin
show the reaction diagram
-
-
-
-
?
GDP-fucose + Galbeta-O-Bn
GDP + L-Fucalpha(1-2)Galbeta-O-Bn
show the reaction diagram
-
-
-
-
?
GDP-fucose + Galbeta1-4Glc
Fucalpha1-2Galbeta1-4Glc + GDP
show the reaction diagram
-
-
-
-
?
GDP-fucose + Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc
Fucalpha1-2Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc + GDP
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + 6-sulfoGalbeta(1-4)GlcNAcbeta-O-methyl
GDP + L-Fucalpha(1-2)6-sulfoGalbeta(1-4)GlcNAcbeta-O-methyl
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + agalacto-asialofetuin
GDP + alpha-L-fucosyl-1,2-agalacto-asialofetuin
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + arabinogalactan protein
?
show the reaction diagram
-
isoforms FUT4.1 and FUT 6 incorporate terminal alpha-L-fucose residues on the C-2 positions of adjacent arabinose residues of arabinogalactan proteins
-
-
?
GDP-L-fucose + asialo alpha1 acid glycoprotein
GDP + alpha-L-fucosyl-1,2-asialo alpha1 acid glycoprotein
show the reaction diagram
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-
-
-
?
GDP-L-fucose + asialofetuin
GDP + alpha-L-fucosyl-1,2-asialofetuin
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + beta-D-galactose-beta-O-2-(2-carbomethoxyethylthio)ethyl
?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + beta-D-galactosyl-R
GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
show the reaction diagram
GDP-L-fucose + beta-methyl-D-galactopyranoside
GDP + alpha-L-fucosyl-1,2-beta-methyl-D-galactopyranoside
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + bovine IgG diantennary glycopeptide
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + D-fucose
GDP + alpha-L-fucosyl-1,2-D-fucose
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + D-galactose
GDP + alpha-L-fucosyl-1,2-D-galactose
show the reaction diagram
GDP-L-fucose + D-Galbeta(1-2)D-Xylalpha(1-6)D-Glcbeta(1-4)D-Glc
GDP + L-Fucalpha(1-2)-D-Galbeta1-2-D-Xylalpha1-6-D-Glcbeta1-4-D-Glc
show the reaction diagram
GDP-L-fucose + di-N-acetylchitobiose
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + fetuin
GDP + alpha-L-fucosyl-1,2-fetuin
show the reaction diagram
GDP-L-fucose + fetuin triantennary asialoglycopeptide
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + galacto-N-biose
GDP + alpha-L-fucosyl-1,2-galacto-N-biose
show the reaction diagram
GDP-L-fucose + Galalpha(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc-ceramide
GDP + L-Fucalpha(1-2)Galalpha(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc-ceramide
show the reaction diagram
-
-
-
?
GDP-L-fucose + GalalphaCH3
GDP + L-Fucalpha(1-2)GalalphaCH3
show the reaction diagram
-
0.1% of the activity with Galbeta1-3GalNAc
-
-
?
GDP-L-fucose + Galbeta(1-2)D-Xyl
GDP + L-Fucalpha(1-2)D-Galbeta(1-2)D-Xyl
show the reaction diagram
GDP-L-fucose + Galbeta(1-3)Ara
GDP + L-Fucalpha(1-2)Galbeta(1-3)Ara
show the reaction diagram
-
3.3% of the activity with Galbeta1-3GalNAc
-
-
?
GDP-L-fucose + Galbeta(1-3)GalNAc
GDP + L-Fucalpha(1-2)Galbeta(1-3)GalNAc
show the reaction diagram
GDP-L-fucose + Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta1-ceramide
GDP + L-Fucalpha(1-2)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta1-cera mide
show the reaction diagram
-
-
-
?
GDP-L-fucose + Galbeta(1-3)GalNAcbeta1-OCH2CH=CH2
GDP + L-Fucalpha(1-2)Galbeta(1-3)GalNAcbeta1-OCH2CH=CH2
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
show the reaction diagram
-
84% of the activity with Galbeta1-3GalNAc
-
-
?
GDP-L-fucose + Galbeta(1-3)GlcNAcbeta-O-allyl/acrylamide copolymer
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta(1-3)GlcNAcbeta-O-benzyl
GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAcbeta-O-benzyl
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta(1-4)Glc
GDP + L-Fucalpha(1-2)Galbeta(1-4)Glc
show the reaction diagram
-
4.2% of the activity with Galbeta1-3GalNAc
-
-
?
GDP-L-fucose + Galbeta(1-4)Glc-beta-O-2-(2-carbomethoxyethylthio)ethyl
GDP + L-Fucalpha(1-2)Galbeta(1-4)Glc-beta-O-2-(2-carbomethoxyethylthio)ethyl
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta(1-4)GlcNAc
GDP + L-Fucalpha(1-2)Galbeta(1-4)GlcNAc
show the reaction diagram
-
7.5% of the activity with Galbeta1-3GalNAc
-
-
?
GDP-L-fucose + Galbeta(1-4)GlcNAc(1-3)Galbeta(1-4)Glc
GDP + L-Fucalpha(1-2)Galbeta(1-4)GlcNAc(1-3)Glabeta(1-4)Glc
show the reaction diagram
-
2.8% of the activity with Galbeta(1-3)GalNAc
-
-
?
GDP-L-fucose + Galbeta(1-4)GlcNAc-beta-O-2-(2-carbomethoxyethylthio)ethyl
GDP + L-Fucalpha(1-2)Galbeta(1-4)Glc-beta-O-2-(2-carbomethoxyethylthio)ethyl
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc-ceramide
GDP + L-Fucalpha(1-2)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc-ceramide
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta(1-4)GlcNAcbeta-O-benzyl
GDP + L-Fucalpha(1-2)Galbeta(1-4)GlcNAcbeta-O-benzyl
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta(1-4)Man
GDP + L-Fucalpha(1-2)Galbeta(1-4)Man
show the reaction diagram
-
2.7% of the activity with Galbeta1-3GalNAc
-
-
?
GDP-L-fucose + Galbeta(1-6)GalNAc
GDP + L-Fucalpha(1-2)Galbeta(1-6)GalNAc
show the reaction diagram
GDP-L-fucose + Galbeta(1->3)-GalNAcalpha-OH
GDP + Fucalpha(1->2)-Galbeta(1->3)-GalNAcalpha-OH
show the reaction diagram
GDP-L-fucose + Galbeta(1->3)-GalNAcalpha-OMe
GDP + Fucalpha(1->2)-Galbeta(1->3)-GalNAcalpha-OMe
show the reaction diagram
GDP-L-fucose + Galbeta-O-benzyl
GDP + L-Fucalpha(1-2)Galbeta-O-benzyl
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + GalbetaCH3
GDP + L-Fucalpha(1-2)GalbetaCH3
show the reaction diagram
-
2.6% of the activity with Galbeta1-3GalNAc
-
-
?
GDP-L-fucose + GM1
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + GM1 ganglioside
GDP + ?
show the reaction diagram
GDP-L-fucose + intestinal glycoprotein
GDP + alpha-L-fucosyl-1,2-intestinal glycoprotein
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + lacto-N-biose 1
GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAc
show the reaction diagram
GDP-L-fucose + lacto-N-fucopentaose II
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + lacto-N-neotetraose
GDP + lacto-N-fucopentaose IV
show the reaction diagram
GDP-L-fucose + lacto-N-tetraose
GDP + lacto-N-fucopentaose I
show the reaction diagram
GDP-L-fucose + lactose
GDP + fucosyllactose
show the reaction diagram
GDP-L-fucose + lactosylceramide
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + N-acetylallolactosamine
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + N-acetyllactosamine
GDP + alpha-L-fucosyl-1,2-N-acetyllactosamine
show the reaction diagram
GDP-L-fucose + p-nitrophenyl beta-D-fucoside
GDP + alpha-L-fucosyl-1,2-nitrophenyl beta-D-fucoside
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + paragloboside
GDP + ?
show the reaction diagram
-
-
-
?
GDP-L-fucose + phenyl-beta-D-galactoside
?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + phenyl-beta-D-galactoside
GDP + phenyl 1-O-(alpha-L-fucopyranosyl)beta-D-galactopyranoside
show the reaction diagram
GDP-L-fucose + porcine submaxillary mucin
GDP + alpha-L-fucosyl-1,2-porcine submaxillary mucin
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
GDP-fucose + Galbeta1-4Glc
Fucalpha1-2Galbeta1-4Glc + GDP
show the reaction diagram
-
-
-
-
?
GDP-fucose + Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc
Fucalpha1-2Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc + GDP
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + beta-D-galactosyl-R
GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
10 mM, enzyme from gastric mucosa: 6.3% of the activation with Mn2+, enzyme from plasma: 108% of the activation with Mn2+
Zn2+
-
stimulates dialyzed enzyme
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2',3'-cyclic GMP
-
slight
2-mercaptoethanol
3-sulfoGalbeta(1,4)GlcNAcbeta(1,6)Galbeta(1,3)GalNAcalpha-O-benzyl
-
-
Ba2+
-
10 mM, complete inhibition
Cd2+
-
10 mM, complete inhibition
Co2+
less than 20% residual activity at 2 mM
dGDP
-
potent
EDTA
-
5 mM, 95% loss of activity
Galbeta(1,3)GalNAcalpha-O-allyl
-
-
-
Galbeta(1,4)GlcNAcbeta(1,6)3-sulfoGalbeta(1,3)-GalNAcalpha-O-benzyl
-
-
-
Galbeta(1,4)GlcNAcbeta(1,6)Galbeta(1,3)-GalNAcalpha-O-benzyl
-
-
-
GDPmannose
-
-
guanosine
-
slight
Hg2+
-
10 mM, complete inhibition
lactose
-
competitive with antifreeze glycoprotein
PCMB
-
-
Triton X-100
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CHAPSO
-
the truncated enzyme is optimally activated by CHAPSO, showing little activity in absence
fucose
-
stimulates
G-3634-A
-
activity is optimal with 5 mg/ml of the cationic detergent
-
GDP-D-mannose
-
stimulates
Triton CF-54
-
the native and the recombinant full-length enzyme display significant activity in absence of detergent or phospholipid and are optimally activated in presence of Triton CF-54 detergent
Triton X-100
-
required
UDP
-
stimulates
UDP-D-glucose
-
stimulates
UDP-N-acetyl-D-glucosamine
-
stimulates
additional information
the enzyme exhibits full activity when 10 mM EDTA is present
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.45
6-sulfoGalbeta(1-4)GlcNAcbeta-O-methyl
-
-
2.9 - 24
beta-D-galactose
3.2 - 8.5
beta-D-galactose-beta-O-2-(2-carbomethoxyethylthio)ethyl
1
bovine IgG diantennary glycopeptide
-
-
-
5.3
D-lactose
at pH 7.6 and 30C
33
di-N-acetylchitobiose
-
-
0.5
fetuin triantennary asialoglycopeptide
-
-
-
3.8 - 11.1
galacto-N-biose
1.5
Galalpha(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc-ceramide
-
-
66
GalalphaCH3
-
-
-
14.6
Galbeta(1-3)Ara
-
-
-
0.28 - 20
Galbeta(1-3)GalNAc
0.61
Galbeta(1-3)GalNAcalpha-O-p-nitrophenol
-
-
12
Galbeta(1-3)GalNAcbeta1-OCH2CH=CH2
-
-
-
0.63
Galbeta(1-3)GlcNAc
-
-
0.17
Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
-
1.6
Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc-beta-O-PA8
-
H-gene encoded enzyme
-
0.31
Galbeta(1-3)GlcNAcbeta-O-allyl/acrylamide copolymer
-
-
-
0.83
Galbeta(1-3)GlcNAcbeta-O-benzoyl
-
-
-
2.9 - 3.1
Galbeta(1-4)Glc-beta-O-2-(2-carbomethoxyethylthio)ethyl
-
2.11
Galbeta(1-4)GlcNAc
-
-
2.5
Galbeta(1-4)GlcNAc-beta-O-2-(2-carbomethoxyethylthio)ethyl
-
H-endoced and Se-encoded enzyme
-
0.6
Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc-ceramide
-
-
0.94
Galbeta(1-4)GlcNAcbeta(1-3)Galbeta1-4Glc
-
-
1.67
Galbeta(1-4)GlcNAcbeta-O-benzyl
-
-
-
1.95
Galbeta(1-6)GalNAc
-
-
-
2.56
Galbeta(1-6)GlcNAc
-
-
0.82
Galbeta-O-benzyl
-
-
-
59.62
Galbeta-O-Me
-
wild-type
10.7
Galbeta1-4Glc
-
-
4.2
Galbeta1-6GalNAc
-
H-gene encoded enzyme
-
18
GalbetaCH3
-
-
-
0.27 - 1
GDP-beta-L-fucose
0.05 - 1.997
GDP-fucose
0.0011 - 1.54
GDP-L-fucose
0.082 - 0.11
GM1
5.6 - 8.3
lacto-N-biose
1.4 - 3.5
lacto-N-biose I
3.4 - 5.5
lacto-N-neotetraose
1.4 - 2.2
lacto-N-tetraose
2.1 - 8.9
lactose
4.9
lactose-N-biose I
-
enzyme from plasma
0.052 - 0.068
lactosylceramide
13.26 - 108
lactulose
1.1 - 14.3
N-acetyl-beta-lactosamine
5.2 - 20
N-acetyllactosamine
8.2 - 8.79
phenyl beta-D-galactoside
25
phenyl beta-galactose
-
enzyme FTA; enzyme FTB
-
3 - 125
phenyl-beta-D-galactoside
2.7 - 8.4
phenyl-beta-O-galactoside
-
additional information
additional information
-
KM-value for asialofetuin: 3.33 mg/ml
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.067
D-lactose
Escherichia coli
A6M9C2
at pH 7.6 and 30C
0.066
GDP-beta-L-fucose
Escherichia coli
A6M9C2
at pH 7.6 and 30C
0.131
GDP-fucose
Escherichia coli
-
-
0.007
GDP-L-fucose
Homo sapiens
-
wild type isoform FUT1, in 50 mM Tris-HCl (pH 7.2), at 37C
0.0497
lactose
Escherichia coli
-
-
0.151
lactulose
Escherichia coli
-
-
0.0173
methyl beta-D-galactopyranoside
Escherichia coli
-
-
0.011
phenyl-beta-D-galactoside
Homo sapiens
-
isoform FUT1 mutant C35T, in 50 mM Tris-HCl (pH 7.2), at 37C; wild type isoform FUT1, in 50 mM Tris-HCl (pH 7.2), at 37C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.013
D-lactose
Escherichia coli
A6M9C2
at pH 7.6 and 30C
3242
0.245
GDP-beta-L-fucose
Escherichia coli
A6M9C2
at pH 7.6 and 30C
651
0.0183
GDP-L-fucose
Homo sapiens
-
wild type isoform FUT1, in 50 mM Tris-HCl (pH 7.2), at 37C
282
0.000088 - 0.000132
phenyl-beta-D-galactoside
4073
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.6
Galbeta(1,3)GalNAcalpha-O-allyl
-
reaction with 6-sulfoGalbeta(1,3)GlcNAcbeta-O-allyl
-
5.9
Galbeta(1,4)GlcNAcbeta(1,6)Galbeta(1,3)-GalNAcalpha-O-benzyl
-
reaction with 6-sulfoGalbeta(1,4)GlcNAcbeta-O-methyl
-
0.05 - 1.41
GDP
0.0125
NEM
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.852
Q5JBG3
activity of WbwK-GST fusion protein with Galbeta(1-3)GalNAcalpha-O-benzyl as substrate; activity of WbwK-GST fusion protein with Galbeta1,3GalNAcalpha-O-benzyl as substrate
0.873
Q5JBG3
activity of WbwK-GST fusion protein with Galbeta(1-3)GalNAcalpha-O-methyl as substrate; activity of WbwK-GST fusion protein with Galbeta1,3GalNAcalpha-O-methyl as substrate
1.141
-
-
129
-
-
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8
-
soluble enzyme
6 - 8
-
enzyme from ovarian tumor
6.4
-
cacodylate-HCl buffer
6.5 - 7
-
-
6.8 - 8
-
-
7.2 - 7.6
-
recombinant enzyme
7.2
-
enzyme from bone marrow
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7.6
-
pH 6.0: 15% of maximal activity, pH 6.4: 59.2% of maximal activity, pH 6.8: 84.8% of maximal activity, pH 7.2-7.6: maximal activity, recombinant enzyme
6.5 - 7.8
-
pH 6.5: about 70% of maximal activity, pH 7.8: about 65% of maximal activity, enzyme from bone marrow
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
at pH 6.0
37
-
assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
16 - 30
-
16C: 61% of maximal activity, 30C: 62% of maximal activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2
calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
stable transfeceted with FUT1 cDNA in a pcDNA3 vector
Manually annotated by BRENDA team
-
gut colonization by symbiotic bacteria stimulates ERK and JNK signal transduction pathways, resulting in activation of transcription factors ATF2 and c-Jun, respectively. This increases transcription of fut2 mRNA and expression of alpha1,2/3-fucosyltransferase activity, resulting in a highly fucosylated intestinal mucosa characteristic of the adult mammalian gut. Blocking the ERK and JNK signaling cascade inhibits the ability of colonization to induce elevated fut2 mRNA and fucosyltransferase activity in the mature colon
Manually annotated by BRENDA team
-
mRNA of FTA and FTC
Manually annotated by BRENDA team
-
down regulation of alpha1,2-fucosyltransferase activity is associated with human gastric tumorigenesis
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
e.g. hepatoma H35 cells, the enzyme is induced during early stages of chemical hepatocarcinogenesis in parenchymal cells of Fischer 344 rats fed a diet supplemented with 0.03% N-2-acetylaminofluorene, undetectable in normal liver tissue
Manually annotated by BRENDA team
-
stable transfeceted with FUT1 cDNA in a pcDNA3 vector
Manually annotated by BRENDA team
-
an enhanced expression of CD173/CD174 and transcription of FUT1 is observed after treatment of human bone marrow endothelial cells (HBMEC) with tumor necrosis factor-alpha (TNF-alpha) or conditioned supernatants of the leukemia cell line KG1a
Manually annotated by BRENDA team
-
undifferentiated leukemic cells, K562, that have erythroid characteristics
Manually annotated by BRENDA team
-
mRNA of FTA and FTC
Manually annotated by BRENDA team
-
prostatic epithelial cells derived from patients with benign prostatic hyperplasia and from patients with prostatic cancer
Manually annotated by BRENDA team
-
mRNA of FTA, FTB and FTC
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
-
x * 50000, enzyme from serum, SDS-PAGE under reducing conditions
57000
-
monomer, under reducing conditions, SDS-PAGE
65000
-
x * 65000, enzyme from gastric mucosa, SDS-PAGE
66000
Q5JBG3
SDS-PAGE; SDS-PAGE
70000
x * 70000, recombinant, His- and pro-protein-tagged enzyme, SDS-PAGE
75000 - 80000
-
gel filtration
110000
-
dimer, under non-reducing conditions, SDS-PAGE
120000
gel filtration
148000
-
gel filtration
150000
-
gel filtration
158000
-
gel filtration
175000
-
trimer, under non-reducing conditions, SDS-PAGE
320000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
monomer
-
1 * 55000 under reducing and non-reducing conditions, 1 * 57000 under reducing conditions, SDS-PAGE
tetramer
-
under non-reducing conditions, SDS-PAGE
trimer
-
under non-reducing conditions, SDS-PAGE
additional information
-
two enzyme species of 55000 Da and 60000 Da detected by SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27
at 27C in 50 mM Tris-HCl buffer pH 7.6, the enzyme shows a half -life of 57 h
100
-
complete inhibition by heating at 100C
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing and thawing has no effect
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 50 mM Tris-HCl buffer pH 7.6, 2 months, 30% loss of activity
-20C, stable for at least 3 months
-
4C, enzyme loses half of its original activity after 36 h
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
enzyme from gastric mucosa
-
enzyme from plasma
-
enzyme from serum
-
glutathione Sepharose column chromatography and Superdex 200 gel filtration
GST-WbsJ fusion protein is purified to homogeneity via GST affinity chromatography followed by gel filtration
-
Ni2+-NTA column chromatography
partial
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloning of gene FTB and FTC
-
enzyme is stably transfected into Chinese hamster ovary cells
-
expressed as full-length protein with and without a C-terminal HPC4-epitope tag in human 293T cells
-
expressed as GST fusion protein in Escherichia coli BL21 (DE3); expressed as GST fusion protein in Escherichia coli BL21 (DE3)
Q5JBG3
expressed in COS-7 cells
-
expressed in Escherichia coli BL21(DE3) and JM109 (DE3) cells
expressed in Nicotiana tabacum BY2 cells
-
expressed in Sus scrofa
-
expression in COS-7 cells
-
expression in NIH 3T3 cells
-
expression of fut1, fut2, and sec1 genes in COS-7 cells
-
full length enzyme and truncated enzyme missing the first 27 amino acid residues from the N-terminus, comprising the cytoplasmic and transmembrane domains
-
porcine endothelial cell line transfected with human alpha(1,2)-fucosyltransferase cDNA
-
subcloned in CHO-K1 cells
-
subcloned in COS-7 or CHOP cells (Chinese hamster ovary cells transformed with Polyoma large T antigen)
-
the GST-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells
the transcription start site of FUT1 changes during differentiation of the erythroid lineage. The tissue-specific and stage-specific expression of the FUT1 are regulated by three distinct promoters. The 5'-flanking region of exon 2 consists of repetitive sequences. The start site of exon 2 is within the long terminal repeat. The repetitive sequences may play a role in the expression of the FUT1
-
the wbsJ gene is overexpressed in Escherichia coli BL21 as a fusion protein with glutathione S-transferase (GST) at its N-terminus
-
transfection of the mouse Fut1 and Fut2, and human FUT1 genes into human ovarian carcinoma-derived RMG-1 cells results in 2030fold increases in cellular alpha1,2-fucosyltransferase activity, and in alteration of the glycolipid composition, including not only fucosylated products, but also precursor glycolipids
-
transfection of the mouse Fut1 and Fut2, and human FUT1 genes into human ovarian carcinoma-derived RMG-1 cells results in 2030fold increases in cellular alpha1,2-fucosyltransferase activity, and in alteration of the glycolipid composition, including not only fucosylated products, but also precursor glycolipids; transfection of the mouse Fut1 and Fut2, and human FUT1 genes into human ovarian carcinoma-derived RMG-1 cells results in 2030fold increases in cellular alpha1,2-fucosyltransferase activity, and in alteration of the glycolipid composition, including not only fucosylated products, but also precursor glycolipids
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme activity is increased in arthritis synovial tissue
-
Fut4 mRNA expression is increased following coculture of epithelial cells with macrophages for 24 h (1.3fold) or 48 h (1.2fold)
-
the enzyme expression is increased in ovarian cancer. Transient overexpression of c-Jun promotes the enzyme expression 3.86 -11.4fold
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H152A
-
Km increased compared to wild-type using substrate lactulose and substrate GDP-fucose
H152R
-
Km increased compared to wild-type using substrate lactulose and substrate GDP-fucose
R154K
-
Km increased compared to wild-type using substrate lactulose and substrate GDP-fucose
R155A
-
Km increased compared to wild-type using substrate lactulose and substrate GDP-fucose
R155K
-
Km increased compared to wild-type using substrate lactulose and substrate GDP-fucose
C35T
-
the mutation is a polymorphism in the Chinese population and does not affect its mRNA transcription, but can slightly decrease the activity of human alpha-(1,2)-fucosyltransferase in vitro
F280S
-
missense polymorphism, almost inactive enzyme, responsible for some non-secretor cases
G247S
-
missense polymorphism, almost inactive enzyme, responsible for some non-secretor case
I129F
-
most frequent nonsense mutation of FUT2 in populations of Asian ancestry
L101F
-
spontaneous missense mutation frequent in Asian populations
S12F
-
the mutation does not significantly decrease the activity of alpha-(1,2)-fucosyltransferase and affect its kinetic property
W143X
-
Nonsense mutation of FUT2 with a allele frequency of 0.46 in populations of European ancestry. Individuals homozygous for the FUT2 nonsecretor genotype appear to be resistant to infection with Norovirus, suggesting that individuals homozygous for nonsecretor status may be unable to mediate host-microbe interactions.
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
synthesis
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