Cloned (Comment) | Organism |
---|---|
the wbsJ gene is overexpressed in Escherichia coli BL21 as a fusion protein with glutathione S-transferase (GST) at its N-terminus | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H152A | Km increased compared to wild-type using substrate lactulose and substrate GDP-fucose | Escherichia coli |
H152R | Km increased compared to wild-type using substrate lactulose and substrate GDP-fucose | Escherichia coli |
R154K | Km increased compared to wild-type using substrate lactulose and substrate GDP-fucose | Escherichia coli |
R155A | Km increased compared to wild-type using substrate lactulose and substrate GDP-fucose | Escherichia coli |
R155K | Km increased compared to wild-type using substrate lactulose and substrate GDP-fucose | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Escherichia coli | |
GDP | - |
Escherichia coli | |
Ni2+ | - |
Escherichia coli | |
Zn2+ | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.106 | - |
GDP-beta-L-fucose | wild-type | Escherichia coli | |
0.462 | - |
GDP-beta-L-fucose | mutant H152R | Escherichia coli | |
0.575 | - |
GDP-beta-L-fucose | mutant R154K | Escherichia coli | |
0.628 | - |
GDP-beta-L-fucose | mutant R155K | Escherichia coli | |
0.865 | - |
GDP-beta-L-fucose | mutant H152A | Escherichia coli | |
1.997 | - |
GDP-beta-L-fucose | mutant R155A | Escherichia coli | |
7.81 | - |
lactose | wild-type | Escherichia coli | |
13.26 | - |
lactulose | wild-type | Escherichia coli | |
55.3 | - |
lactulose | mutant H152A | Escherichia coli | |
59.62 | - |
Galbeta-O-Me | wild-type | Escherichia coli | |
64.8 | - |
lactulose | mutant R155K | Escherichia coli | |
76 | - |
lactulose | mutant R155A | Escherichia coli | |
96.7 | - |
lactulose | mutant H152R | Escherichia coli | |
108 | - |
lactulose | mutant R154K | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | activity is found to be independent of divalent metal ions | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
GST-WbsJ fusion protein is purified to homogeneity via GST affinity chromatography followed by gel filtration | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
relative activity: lactose: 100%, Galbeta(1,4)Glcbeta-N3 (LacN3): 137%, Galbeta(1,4)Glcbeta-O-phenyl: 84.7%, Galbeta(1,4)Glcbeta-S-phenyl: 207%, Galbeta(1,4Glc)beta-1-NAc: 199%, Galbeta-p-nitrophenol: 30.4%, galactose: 35.7%, Galbeta-O-Me: 68.8%, Galbeta(1,4)GlcNAc: 12.4%, Galbeta(1,4)Fru: 380%, Galbeta(1,4)Man: 162%, Galbeta(1,3)GalNAcalpha-O-benzyl: 202%, Galbeta(1,3)GalNAcalpha-O-Me: 215% | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-beta-L-fucose + galactose | - |
Escherichia coli | GDP + ? | - |
? | |
GDP-beta-L-fucose + Galbeta(1,3)GalNAcalpha-O-Me | - |
Escherichia coli | GDP + ? | - |
? | |
GDP-beta-L-fucose + Galbeta(1,3)GalNAcalpha-O-phenyl | - |
Escherichia coli | GDP + ? | - |
? | |
GDP-beta-L-fucose + Galbeta(1,4)Glcbeta-1-NAc | - |
Escherichia coli | GDP + ? | - |
? | |
GDP-beta-L-fucose + Galbeta(1,4)Glcbeta-N3 | - |
Escherichia coli | GDP + ? | - |
? | |
GDP-beta-L-fucose + Galbeta(1,4)Glcbeta-O-phenyl | - |
Escherichia coli | GDP + ? | - |
? | |
GDP-beta-L-fucose + Galbeta(1,4)Glcbeta-S-phenyl | - |
Escherichia coli | GDP + ? | - |
? | |
GDP-beta-L-fucose + Galbeta(1,4)Man | - |
Escherichia coli | GDP + ? | - |
? | |
GDP-beta-L-fucose + Galbeta(1-3)GalNAc | - |
Escherichia coli | GDP + L-Fucalpha(1-2)Galbeta(1-3)GalNAc | - |
? | |
GDP-beta-L-fucose + Galbeta-O-Me | - |
Escherichia coli | GDP + L-Fucalpha(1-2)Galbeta-O-Me | - |
? | |
GDP-beta-L-fucose + Galbeta-p-nitrophenol | - |
Escherichia coli | GDP + L-Fucalpha(1-2)Galbeta-p-nitrophenol | - |
? | |
GDP-beta-L-fucose + lactose | - |
Escherichia coli | GDP + fucosyllactose | - |
? | |
GDP-beta-L-fucose + lactulose | - |
Escherichia coli | GDP + ? | - |
? | |
GDP-beta-L-fucose + lactulose | - |
Escherichia coli | GDP + 2'-fucosyl-D-lactulose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | gel filtration, 2 * 60000 Da | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
alpha1,2-fucosyltransferase | - |
Escherichia coli |
WbsJ | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0173 | - |
methyl beta-D-galactopyranoside | - |
Escherichia coli | |
0.0497 | - |
lactose | - |
Escherichia coli | |
0.131 | - |
GDP-beta-L-fucose | - |
Escherichia coli | |
0.151 | - |
lactulose | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.41 | - |
GDP | - |
Escherichia coli |