Information on EC 1.1.3.9 - galactose oxidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.3.9
-
RECOMMENDED NAME
GeneOntology No.
galactose oxidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-galactose + O2 = D-galacto-hexodialdose + H2O2
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Galactose metabolism
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-
SYSTEMATIC NAME
IUBMB Comments
D-galactose:oxygen 6-oxidoreductase
A copper protein.
CAS REGISTRY NUMBER
COMMENTARY hide
9028-79-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Fusarium moniliforme f. sp. subglutinans
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
i.e. Gibberella pulicaris
UniProt
Manually annotated by BRENDA team
i.e. Gibberella pulicaris
UniProt
Manually annotated by BRENDA team
expression in Pichia pastoris
-
-
Manually annotated by BRENDA team
gene gaoA
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
gene gaoA
UniProt
Manually annotated by BRENDA team
gene gaoA
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Polyporus circinatus
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
-
deletion of domain 1 completely abolishes the enzyme activity and is thus speculated to be important also for the correct folding of domain 2
physiological function
-
galactose oxidase catalyzes the 2e- oxidation of primary alcohols to aldehydes and the enzyme may also be capable of the subsequent slower 2e- oxidation to carboxylic acids. Each of these reactions is coupled to the 2e- reduction of O2 to H2O2. The native substrate of the enzyme is D-galactose, but a broad range of other sugar and aromatic alcohol substrates are also active, which has led to the proposal that the physiological role of GO is the generation of H2O2, perhaps as a defense against pathogenic organisms
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,5-anhydrogalactitol + O2
? + H2O2
show the reaction diagram
Polyporus circinatus
-
-
-
?
1-methyl-alpha-D-galactopyranoside + O2
1-O-methyl-alpha-D-galacto-hexodialdose + H2O2
show the reaction diagram
1-methyl-alpha-D-galactopyranoside + O2
? + H2O2
show the reaction diagram
in the oxidations of methyl-alpha-D-galactopyranoside and methyl-beta-D-galactopyranoside, a dimeric product, a water elimination product, and an alpha,beta-unsaturated aldehyde occur among the mix of products. In the case of oxidized beta-galactose, the unsaturated aldehyde likely forms in the reaction
-
-
?
1-methyl-beta-D-galactopyranoside + O2
? + H2O2
show the reaction diagram
1-O-methyl-alpha-D-galactopyranoside + O2
1-O-methyl-alpha-D-galacto-hexodialdose + H2O2
show the reaction diagram
-
-
-
-
?
1-O-methyl-alpha-D-galactosylpyranoside + O2
1-O-methyl-alpha-D-galacto-hexodialdose + H2O2
show the reaction diagram
1-O-methyl-alpha-D-glucosylpyranoside + O2
1-O-methyl-alpha-D-gluco-hexodialdose + H2O2
show the reaction diagram
1-O-methyl-beta-D-galactosylpyranoside + O2
1-O-methyl-beta-D-galacto-hexodialdose + H2O2
show the reaction diagram
1-O-methyl-beta-D-glucosylpyranoside + O2
1-O-methyl-beta-D-gluco-hexodialdose + H2O2
show the reaction diagram
1-O-methyl-D-galactopyranoside + O2
?
show the reaction diagram
-
112% of the activity with D-galactose
-
-
?
2 raffinose + 2 O2
6''-aldehydoraffinose + 6''-carboxyraffinose + H2O2 + H2O
show the reaction diagram
2-deoxy-D-galactose + O2
2-deoxy-D-galacto-hexodialdose + H2O2
show the reaction diagram
2-deoxy-D-galactose + O2
?
show the reaction diagram
-
-
-
?
2-glycerol-alpha-D-galactosylpyranoside + O2
2-glycerol-alpha-D-galactosyl-hexodialdose + H2O2
show the reaction diagram
Polyporus circinatus
-
low activity
-
?
2-methylene-1,3-propanediol + O2
?
show the reaction diagram
-
-
-
?
3-bromo-1,2-propanediol + O2
? + H2O2
show the reaction diagram
-
-
S isomer
?
3-bromobenzyl alcohol + O2
3-bromobenzaldehyde + H2O2
show the reaction diagram
-
-
-
?
3-chloro-1,2-propanediol + O2
? + H2O2
show the reaction diagram
-
only R isomer will have the correct orientation to react with the enzyme
S isomer
?
3-chlorobenzyl alcohol + O2
3-chlorobenzaldehyde + H2O2
show the reaction diagram
-
-
-
?
3-fluoro-1-phenylethanol + O2
?
show the reaction diagram
-
-
-
-
?
3-fluorobenzyl alcohol + O2
3-fluorobenzaldehyde + H2O2
show the reaction diagram
-
-
-
?
3-methoxybenzyl alcohol + O2
3-methoxybenzaldehyde + H2O2
show the reaction diagram
-
-
-
?
3-methoxybenzyl alcohol + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
3-nitrobenzyl alcohol + O2
3-nitrobenzaldehyde + H2O2
show the reaction diagram
-
-
-
?
4-(methylthio)benzyl alcohol + O2
4-(methylthio)benzaldehyde + H2O2
show the reaction diagram
-
-
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?
4-(trifluoromethyl)benzyl alcohol + O2
4-(trifluoromethyl)benzaldehyde + H2O2
show the reaction diagram
-
-
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?
4-bromobenzyl alcohol + O2
4-bromobenzaldehyde + H2O2
show the reaction diagram
-
-
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?
4-chlorobenzyl alcohol + O2
4-chlorobenzaldehyde + H2O2
show the reaction diagram
-
-
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?
4-fluorobenzyl alcohol + O2
4-fluorobenzaldehyde + H2O2
show the reaction diagram
-
-
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?
4-iodobenzyl alcohol + O2
4-iodobenzaldehyde + H2O2
show the reaction diagram
-
-
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?
4-methoxybenzyl alcohol + O2
4-methoxybenzaldehyde + H2O2
show the reaction diagram
-
-
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?
4-methoxybenzyl alcohol + O2
? + H2O2
show the reaction diagram
-
-
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?
4-methylbenzyl alcohol + O2
4-methylbenzaldehyde + H2O2
show the reaction diagram
-
-
-
?
4-nitrobenzyl alcohol + O2
4-nitrobenzaldehyde + H2O2
show the reaction diagram
-
-
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?
4-nitrobenzyl alcohol + O2
? + H2O2
show the reaction diagram
-
-
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?
4-O-beta-D-glucopyranosyl-D-glucose + O2
4-O-beta-D-glucopyranosyl-D-gluco-hexodialdose + H2O2
show the reaction diagram
acetol + O2
pyruvaldehyde + H2O2
show the reaction diagram
-
-
-
?
allyl alcohol + O2
acrolein + H2O2
show the reaction diagram
-
only extracelluar enzyme, low activity
-
?
alpha-D-talose + O2
alpha-D-talo-hexodialdose + H2O2
show the reaction diagram
benzene-1,2-diamine + O2
? + H2O2
show the reaction diagram
-
very low activity
-
?
benzyl alcohol + O2
? + H2O2
show the reaction diagram
benzyl alcohol + O2
benzaldehyde + H2O2
show the reaction diagram
benzylalcohol + O2
benzaldehyde + H2O2
show the reaction diagram
-
substrate reaction profiling
-
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?
beta-D-galactopyranosyl-(1-6)-beta-D-galactopyranosyl-(1-4)-D-glucose + O2
? + H2O2
show the reaction diagram
Polyporus circinatus
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?
beta-D-galactosyl-(1-6)-beta-D-galactopyranoside + O2
? + H2O2
show the reaction diagram
Polyporus circinatus
-
-
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?
beta-D-lactose + O2
beta-D-lacto-hexodialdose + H2O2
show the reaction diagram
beta-hydroxypyruvate + O2
2,3-dioxopropionate + H2O2
show the reaction diagram
-
only extracellular enzyme, low activity
-
?
beta-thiodigalactoside + O2
? + H2O2
show the reaction diagram
Polyporus circinatus
-
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?
beta-thiogalactoside + O2
beta-thiogalacto-hexodialdose + H2O2
show the reaction diagram
Polyporus circinatus
-
more rapidly oxidized than beta-D-galactose
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?
ceramide dihexoside + O2
? + H2O2
show the reaction diagram
-
higher activity than free substrate, very low activity as vesicle-bound substrate
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?
ceramide trihexoside + O2
? + H2O2
show the reaction diagram
-
vesicle-bound and free substrate
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?
corn arabinoxylan + O2
?
show the reaction diagram
-
-
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?
D-Gal-beta-(1-3)-D-Gal-beta-(1-1)-L-Gro + O2
? + H2O2
show the reaction diagram
-
low activity
-
?
D-Gal-beta-(1-3)-D-Gal-beta-(1-3)-D-Gal-(1-1)-L-Gro + O2
? + H2O2
show the reaction diagram
-
low activity
-
?
D-Gal-beta-(1-3)-D-Gal-beta-(1-6)-D-Gal-beta-(1-1)-L-Gro + O2
? + H2O2
show the reaction diagram
-
lower activity than with a reversed beta-1-6-linkage
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?
D-Gal-beta-(1-3)-[D-Gal-beta-(1-6)]-D-Gal-beta-(1-1)-L-Gro + O2
? + H2O2
show the reaction diagram
-
best oligosaccharide oxidized
-
?
D-Gal-beta-(1-6)-D-Gal-beta-(1-1)-L-Gro + O2
? + H2O2
show the reaction diagram
-
faster oxidation than corresponding beta-1-3-linked components
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?
D-Gal-beta-(1-6)-D-Gal-beta-(1-3)-D-Gal-beta-(1-1)-L-Gro + O2
? + H2O2
show the reaction diagram
-
improved activity
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?
D-galactopyranose + ferricyanide
D-galacto-hexodialdose + ferrocyanide
show the reaction diagram
-
ferricyanide poorly replaces O2 as electron acceptor
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?
D-galactosamine + O2
? + H2O2
show the reaction diagram
D-galactose + O2
D-galacto-hexodialdose + H2O2
show the reaction diagram
D-galactosylpyranoside + O2
D-galacto-hexodialdose + H2O2
show the reaction diagram
D-glucosylpyranoside + O2
D-gluco-hexodialdose + H2O2
show the reaction diagram
-
very low activity
-
?
D-xylose + O2
? + H2O2
show the reaction diagram
-
very low activity
-
?
dihydroxyacetone + O2
3-hydroxy-2-oxo-propionaldehyde + H2O2
show the reaction diagram
dihydroxyacetone + O2
?
show the reaction diagram
-
-
-
-
?
fetuin + O2
? + H2O2
show the reaction diagram
-
bovine fetuin, native or desialylated
-
?
Forssman glycolipid + O2
? + H2O2
show the reaction diagram
-
higher activity as vesicle-bound substrate, very low activity as free substrate
-
?
Gal-beta-(1-3)-[Fuc-alpha-(1-2)]-GalNAcol + O2
? + H2O2
show the reaction diagram
-
no oxidation of oligosaccharides containing N-acetylgalactosamine at the non-reducing end
-
?
galactan + O2
? + H2O2
show the reaction diagram
-
derived from snail, Lymnea stagnalis galactan best substrate
-
?
galactogen + O2
? + H2O2
show the reaction diagram
-
substrate from Helix pomatia, galactose oxidase acts upon a specific subterminal nonreducing D-galactosyl residue
-
?
galactoglucomannan + O2
?
show the reaction diagram
-
-
-
?
galactolipid + O2
? + H2O2
show the reaction diagram
-
-
-
?
galactose 1-phosphate + O2
? + H2O2
show the reaction diagram
Polyporus circinatus
-
very low activity
-
?
galactose-4-SO3Na + O2
? + H2O2
show the reaction diagram
-
very low activity
-
?
galactose-6-SO3Na + O2
? + H2O2
show the reaction diagram
-
very low activity
-
?
galactoxyloglucan + O2
?
show the reaction diagram
-
-
-
?
ganglioside + O2
? + H2O2
show the reaction diagram
globoside + O2
? + H2O2
show the reaction diagram
-
human and porcine globoside, vesicle-bound and free substrate, best substrate tested
-
?
glyceraldehyde + O2
? + H2O2
show the reaction diagram
-
70% of the activity with glycolaldehyde
-
-
?
glycerol + O2
(S)-glyceraldehyde + H2O2
show the reaction diagram
glycoaldehyde + O2
glyoxal + H2O2
show the reaction diagram
-
only extracellular enzyme, low activity
-
?
glycolaldehyde + O2
glyoxal + H2O2
show the reaction diagram
glycoprotein + O2
? + H2O2
show the reaction diagram
-
-
-
?
guar + O2
? + H2O2
show the reaction diagram
guar galactomannan + O2
?
show the reaction diagram
-
-
-
?
guar gum + O2
? + H2O2
show the reaction diagram
-
significant activity
-
?
guaran + O2
? + H2O2
show the reaction diagram
Helix pomatia galactomannan + O2
?
show the reaction diagram
-
-
-
?
hexadecyl-(ethyleneglycol)13-D-galactose + O2
hexadecyl-(ethyleneglycol)13-D-galacto-hexodialdose + H2O2
show the reaction diagram
-
-
-
-
?
hexadecyl-(ethyleneglycol)20-D-galactose + O2
hexadecyl-(ethyleneglycol)20-D-galacto-hexodialdose + H2O2
show the reaction diagram
-
-
-
-
?
hexadecyl-(ethyleneglycol)6-D-galactose + O2
hexadecyl-(ethyleneglycol)6-D-galacto-hexodialdose + H2O2
show the reaction diagram
-
-
-
-
?
hexadecyl-(ethyleneglycol)9-D-galactose + O2
hexadecyl-(ethyleneglycol)9-D-galacto-hexodialdose + H2O2
show the reaction diagram
-
-
-
-
?
isopropyl-beta-D-thiogalactosylpyranoside + O2
isopropyl-beta-D-thiogalactosyl-hexodialdose + H2O2
show the reaction diagram
Polyporus circinatus
-
43% of the activity compared to D-galactose
-
?
L-glucose + O2
L-gluco-hexodialdose + H2O2
show the reaction diagram
-
very low activity
-
?
lactitol + O2
?
show the reaction diagram
-
-
-
?
lactobionic acid + O2
?
show the reaction diagram
-
-
-
?
lactose + O2
?
show the reaction diagram
lactose + O2
? + H2O2
show the reaction diagram
lactulose + O2
?
show the reaction diagram
-
-
-
?
lactylamine + O2
?
show the reaction diagram
-
-
-
?
larch arabinogalactan + O2
?
show the reaction diagram
-
-
-
?
locust bean galactomannan + O2
?
show the reaction diagram
-
-
-
?
maltose + O2
? + H2O2
show the reaction diagram
-
very low activity
-
?
melibiitol + O2
? + H2O2
show the reaction diagram
Polyporus circinatus
-
-
-
?
melibionic acid + O2
? + H2O2
show the reaction diagram
Polyporus circinatus
-
low activity
-
?
melibiose + O2
?
show the reaction diagram
melibiose + O2
? + H2O2
show the reaction diagram
methyl alpha-D-galactopyranoside + O2
methyl alpha-D-galacto-hexodialdo-1,5-pyranoside + H2O2
show the reaction diagram
-
investigation of the optimal reaction conditions (reaction medium, temperature, concentration and combinations of galactose oxidase, catalase, and horseradish peroxidase are used as variables) to degrade methyl alpha-D-galactopyranoside to alpha-D-galacto-hexodialdo-1,5-pyranoside and thereby reduce byproduct formation. Optimal combination of the 3 enzymes gives methyl alpha-D-galacto-hexodialdo-1,5-pyranoside in approximately 90% yield
-
-
?
methyl alpha-D-galactopyranoside + O2
methyl alpha-D-galacto-hexodialdose + H2O2
show the reaction diagram
-
-
-
-
?
methyl beta-D-mannopyranoside + O2
?
show the reaction diagram
-
-
-
?
methyl beta-D-thiogalactosylpyranoside + O2
methyl beta-D-thiogalacto-hexodialdose + H2O2
show the reaction diagram
Polyporus circinatus
-
-
-
?
mucin + O2
? + H2O2
show the reaction diagram
-
bovine submaxillary mucin, native and desialylated
-
-
N-acetyl-D-galactosamine + O2
? + H2O2
show the reaction diagram
N-acetyllactosamine + O2
?
show the reaction diagram
-
-
-
?
o-nitrophenyl beta-D-galactoside + O2
1-O-(o-nitrophenyl)-alpha-D-galactohexodialdose + H2O2
show the reaction diagram
p-nitrophenyl alpha-D-galactoside + O2
1-O-(p-nitrophenyl)-alpha-D-galactohexodialdose + H2O2
show the reaction diagram
-
more reactive than p-nitrophenyl-beta-D-galactoside
-
?
p-nitrophenyl beta-D-galactoside + O2
1-O-(p-nitrophenyl)-beta-D-galactohexodialdose + H2O2
show the reaction diagram
-
less reactive than p-nitrophenyl-alpha-D-galactoside
-
?
planteose + O2
? + H2O2
show the reaction diagram
Polyporus circinatus
-
-
-
?
raffinose + O2
6''-aldehydoraffinose + 6''-carboxyraffinose + H2O2 + H2O
show the reaction diagram
raffinose + O2
?
show the reaction diagram
raffinose + O2
? + H2O2
show the reaction diagram
raffinose + O2
P: 6''-aldehydoraffinose + 6''-carboxyraffinose + H2O2 + H2O
show the reaction diagram
-
-
-
?
sphingoglycolipid + O2
? + H2O2
show the reaction diagram
-
-
-
?
spruce galactoglucomannan + O2
?
show the reaction diagram
-
-
-
?
stachyose + O2
? + H2O2
show the reaction diagram
sucrose + O2
? + H2O2
show the reaction diagram
-
very low activity
-
?
talose + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
tamarind galactoxyloglucan + O2
?
show the reaction diagram
-
-
-
?
xyloglucan + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 raffinose + 2 O2
6''-aldehydoraffinose + 6''-carboxyraffinose + H2O2 + H2O
show the reaction diagram
2-deoxy-D-galactose + O2
2-deoxy-D-galacto-hexodialdose + H2O2
show the reaction diagram
4-O-beta-D-glucopyranosyl-D-glucose + O2
4-O-beta-D-glucopyranosyl-D-gluco-hexodialdose + H2O2
show the reaction diagram
alpha-D-talose + O2
alpha-D-talo-hexodialdose + H2O2
show the reaction diagram
beta-D-galactopyranosyl-(1-6)-beta-D-galactopyranosyl-(1-4)-D-glucose + O2
? + H2O2
show the reaction diagram
Polyporus circinatus
-
-
-
?
beta-D-galactosyl-(1-6)-beta-D-galactopyranoside + O2
? + H2O2
show the reaction diagram
Polyporus circinatus
-
-
-
?
beta-D-lactose + O2
beta-D-lacto-hexodialdose + H2O2
show the reaction diagram
ceramide dihexoside + O2
? + H2O2
show the reaction diagram
-
higher activity than free substrate, very low activity as vesicle-bound substrate
-
?
ceramide trihexoside + O2
? + H2O2
show the reaction diagram
-
vesicle-bound and free substrate
-
?
D-galactosamine + O2
? + H2O2
show the reaction diagram
D-galactose + O2
D-galacto-hexodialdose + H2O2
show the reaction diagram
D-galactosylpyranoside + O2
D-galacto-hexodialdose + H2O2
show the reaction diagram
D-glucosylpyranoside + O2
D-gluco-hexodialdose + H2O2
show the reaction diagram
-
very low activity
-
?
D-xylose + O2
? + H2O2
show the reaction diagram
-
very low activity
-
?
dihydroxyacetone + O2
3-hydroxy-2-oxo-propionaldehyde + H2O2
show the reaction diagram
fetuin + O2
? + H2O2
show the reaction diagram
-
bovine fetuin, native or desialylated
-
?
Forssman glycolipid + O2
? + H2O2
show the reaction diagram
-
higher activity as vesicle-bound substrate, very low activity as free substrate
-
?
Gal-beta-(1-3)-[Fuc-alpha-(1-2)]-GalNAcol + O2
? + H2O2
show the reaction diagram
-
no oxidation of oligosaccharides containing N-acetylgalactosamine at the non-reducing end
-
?
galactolipid + O2
? + H2O2
show the reaction diagram
-
-
-
?
ganglioside + O2
? + H2O2
show the reaction diagram
globoside + O2
? + H2O2
show the reaction diagram
-
human and porcine globoside, vesicle-bound and free substrate, best substrate tested
-
?
glycoprotein + O2
? + H2O2
show the reaction diagram
-
-
-
?
guar gum + O2
? + H2O2
show the reaction diagram
-
significant activity
-
?
guaran + O2
? + H2O2
show the reaction diagram
maltose + O2
? + H2O2
show the reaction diagram
-
very low activity
-
?
melibiose + O2
? + H2O2
show the reaction diagram
mucin + O2
? + H2O2
show the reaction diagram
-
bovine submaxillary mucin, native and desialylated
-
-
N-acetyl-D-galactosamine + O2
? + H2O2
show the reaction diagram
planteose + O2
? + H2O2
show the reaction diagram
Polyporus circinatus
-
-
-
?
raffinose + O2
? + H2O2
show the reaction diagram
sphingoglycolipid + O2
? + H2O2
show the reaction diagram
-
-
-
?
stachyose + O2
? + H2O2
show the reaction diagram
sucrose + O2
? + H2O2
show the reaction diagram
-
very low activity
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cys-Tyr cofactor
-
pyrroloquinoline quinone
-
one pyrroquinoline covalently bound to one molecule, additional two-electron redox center
additional information
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
copper
Cu3+
-
first evidence of oxidation by the Cu(III) enzyme with concomitant formation of a Cu(I) enzyme
Fe
-
1 atom iron per mol enzyme
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,2'-dipyridyl
-
1 mM, 67% inhibition
2,4,6-Trinitrobenzenesulfonic acid
-
53% inhibition at 1 mM
2-deoxy-D-galactose
Polyporus circinatus
-
competitive inhibition
2-mercaptoethanol
8-hydroxyquinoline
-
1 mM, 50% inhibition
AgNO3
-
complete inhibition at 0.1 mM
Co2+
-
1 mM, 50% inhibition
D-galactono-1,4-lactone
-
23.7% inhibition at 0.25 mM
D-galactosamine
Polyporus circinatus
-
competitive inhibition
D-galacturonic acid
-
66.8% inhibition at 0.25 mM
diethyldithiocarbamate
dithiothreitol
-
40% inhibition at 1 mM
Fe2+
-
1 mM, 17% inhibition
ferricyanide
-
inhibition at 0.001 mM, activation at 1 mM
ganglioside
Polyporus circinatus
-
bovine brain and Tay-Sachs disease gangliosides: competitive inhibition
GSH
-
complete inhibition at 10 mM
hydrazine
-
1 mM, 41% inhibition
hydroxylamine
iodoacetamide
iodoacetic acid
-
slight inactivation
KCN
complete inhibition
L-ascorbic acid
-
58% inhibition at 0.1 mM
Mg2+
-
1 mM, 50% inhibition
Monoiodoacetic acid
-
1 mM, 40% inhibition
N-acetylgalactosamine
Polyporus circinatus
-
competitive inhibition
N-bromosuccinimide
NH2OH
-
complete inhibition at 0.1 mM
Ni2+
-
1 mM, 50% inhibition
o-phenanthroline
p-chloromercuribenzoate
-
22% inhibition at 0.1 mM
phenylhydrazine
-
1 mM, 91% inhibition
Semicarbazide
-
1 mM, 44% inhibition
Sodium dithionite
-
73% inhibition at 0.1 mM
Superoxide dismutase
-
50% inhibition at 50 pM
-
trans-(2)-phenylcyclopropylcarbinol
-
mechanism-based inactivator
Zn2+
-
1 mM, 33% inhibition
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
chloroiridate
-
marked increase of activity
copper sulfate
Coprimus cinereus peroxidase
-
13fold activation
-
EDTA
-
up to 7fold activation
ferricyanide
hexacyanoferrate (III)
-
iridium (IV) chloride
-
K3[Fe(CN)6]
molybdic cyanide
-
phosphate
-
-
potassium dichromate
-
Sodium periodate
superoxide
-
stimulation of O2 uptake
Tween 80
slight activation
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
16 - 56
1-methyl-alpha-D-galactopyranoside
77 - 440
1-O-methyl-alpha-D-galactopyranoside
29 - 57
2-methylene-1,3-propanediol
0.0021 - 2950
D-galactose
0.074 - 0.16
galactoglucomannan
0.07 - 0.14
galactoxyloglucan
-
7.5
glyceraldehyde
-
-
13.2
glycolaldehyde
-
-
0.037 - 0.22
guar galactomannan
-
0.008 - 0.19
locust bean galactomannan
-
additional information
additional information
-
steady-state kinetics, isotope kinetics, kinetic analysis
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
457 - 1548
1-methyl-alpha-D-galactopyranoside
325
1-O-methyl beta-D-galactopyranoside
-
Cu(II)-bound H2O is required in the protonated aquo state for catalysis and is responsible for the kinetic solvent isotope effect
20 - 1165
1-O-methyl-alpha-D-galactopyranoside
1440
1-O-methyl-beta-D-galactopyranoside
-
-
166 - 283
2-methylene-1,3-propanediol
0.011 - 3400
D-galactose
3.25 - 3.98
galactoglucomannan
3.65 - 4.57
galactoxyloglucan
-
13.7 - 58
guar
-
5.18 - 6.7
guar galactomannan
-
3.37 - 4.3
locust bean galactomannan
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.2
1-methyl-beta-D-galactopyranoside
pH 7.0, 40C
-
0.00019 - 32
D-galactose
20.31 - 45.05
galactoglucomannan
23.02 - 60.09
galactoxyloglucan
-
23.56 - 181.08
guar galactomannan
-
22.63 - 445.8
locust bean galactomannan
-
2700
melibiose
recombinant His-tagged enzyme, pH 7.0, 30C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.3
2-deoxy-D-galactose
Polyporus circinatus
-
-
0.073
D-galactosamine
Polyporus circinatus
-
-
0.002 - 0.12
ganglioside
5.7
iodoacetamide
-
pseudo-first-order kinetics, pH-independent
0.047
N-acetylgalactosamine
Polyporus circinatus