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Literature summary for 1.1.3.9 extracted from

  • Baron, A.J.; Stevens, C.; Wilmot, C.; Seneviratne, K.D.; Blakeley, V.; Dooley, D.M.; Phillips, S.E.V.; Knowles, P.F.; McPherson, M.J.
    Structure and mechanism of galactose oxidase. The free radical site (1994), J. Biol. Chem., 269, 25095-25105.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Aspergillus nidulans Hypomyces rosellus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion, native, recombinant and mutant enzymes Hypomyces rosellus

Protein Variants

Protein Variants Comment Organism
C228G analysis from X-ray structure Hypomyces rosellus
W290H analysis from X-ray structure Hypomyces rosellus
Y272F not capable of binding copper Hypomyces rosellus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 1.2 mol per mol enzyme Hypomyces rosellus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
1 * 65000, SDS-PAGE Hypomyces rosellus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galactosylpyranoside + O2 Hypomyces rosellus
-
D-galacto-hexodialdose + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Hypomyces rosellus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Hypomyces rosellus
to homogeneity, 1step chromatography Hypomyces rosellus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Hypomyces rosellus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.035
-
mutant C228G Hypomyces rosellus
0.54
-
mutant W290H Hypomyces rosellus
1093
-
recombinant wild-type enzyme Hypomyces rosellus
1120
-
native wild-type enzyme Hypomyces rosellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactosylpyranoside + O2
-
Hypomyces rosellus D-galacto-hexodialdose + H2O2
-
?

Subunits

Subunits Comment Organism
monomer 1 * 65000, SDS-PAGE Hypomyces rosellus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.795
-
D-galactose mutant C228G Hypomyces rosellus
2.45
-
D-galactose mutant W290H Hypomyces rosellus
2.98
-
D-galactose native wild-type enzyme Hypomyces rosellus
3.42
-
D-galactose recombinant wild-type enzyme Hypomyces rosellus