Cloned (Comment) | Organism |
---|---|
expression in Aspergillus nidulans | Hypomyces rosellus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapour diffusion, native, recombinant and mutant enzymes | Hypomyces rosellus |
Protein Variants | Comment | Organism |
---|---|---|
C228G | analysis from X-ray structure | Hypomyces rosellus |
W290H | analysis from X-ray structure | Hypomyces rosellus |
Y272F | not capable of binding copper | Hypomyces rosellus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1.2 mol per mol enzyme | Hypomyces rosellus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
1 * 65000, SDS-PAGE | Hypomyces rosellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactosylpyranoside + O2 | Hypomyces rosellus | - |
D-galacto-hexodialdose + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hypomyces rosellus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Hypomyces rosellus |
to homogeneity, 1step chromatography | Hypomyces rosellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Hypomyces rosellus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.035 | - |
mutant C228G | Hypomyces rosellus |
0.54 | - |
mutant W290H | Hypomyces rosellus |
1093 | - |
recombinant wild-type enzyme | Hypomyces rosellus |
1120 | - |
native wild-type enzyme | Hypomyces rosellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactosylpyranoside + O2 | - |
Hypomyces rosellus | D-galacto-hexodialdose + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 65000, SDS-PAGE | Hypomyces rosellus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.795 | - |
D-galactose | mutant C228G | Hypomyces rosellus | |
2.45 | - |
D-galactose | mutant W290H | Hypomyces rosellus | |
2.98 | - |
D-galactose | native wild-type enzyme | Hypomyces rosellus | |
3.42 | - |
D-galactose | recombinant wild-type enzyme | Hypomyces rosellus |