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Literature summary for 1.1.3.9 extracted from

  • Rogers, M.S.; Hurtado-Guerrero, R.; Firbank, S.J.; Halcrow, M.A.; Dooley, D.M.; Phillips, S.E.; Knowles, P.F.; McPherson, M.J.
    Cross-link formation of the cysteine 228-tyrosine 272 catalytic cofactor of galactose oxidase does not require dioxygen (2008), Biochemistry, 47, 10428-10439.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of galactose oxidase without the pro sequence. Reaction with excess Cu2+ leads to formation of the C228-Y272 thioether bond both under aerobic and anaerobic conditions, the formation of the Y272 radical occures solely under aerobic conditions (posttranslational modifications) Fusarium graminearum

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop method. 2-5 mg/ml of enzyme mixed with equal volume of well solution. Crystals are soaked in oxygen-free Cu2+ solutions Fusarium graminearum

Inhibitors

Inhibitors Comment Organism Structure
EDTA inhibits the formation of the thioether bond under anaerobic conditions (posttranslational modification) Fusarium graminearum

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+
-
Fusarium graminearum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68500
-
recombinant galactose oxidase without the pro sequence, SDS-PAGE Fusarium graminearum

Organism

Organism UniProt Comment Textmining
Fusarium graminearum P0CS93
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification cleavage of a secretion signal sequence, copper-dependent cleavage of an N-terminal pro sequence Fusarium graminearum
side-chain modification copper-dependent formation of a C228-Y272 thioether bond, generation of the Y272 radical Fusarium graminearum

Purification (Commentary)

Purification (Comment) Organism
purification of recombinant galactose oxidase under strict copper-free conditions Fusarium graminearum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + O2
-
Fusarium graminearum D-galacto-hexodialdose + H2O2
-
?

Synonyms

Synonyms Comment Organism
galactose oxidase
-
Fusarium graminearum