Cloned (Comment) | Organism |
---|---|
recombinant expression of galactose oxidase without the pro sequence. Reaction with excess Cu2+ leads to formation of the C228-Y272 thioether bond both under aerobic and anaerobic conditions, the formation of the Y272 radical occures solely under aerobic conditions (posttranslational modifications) | Fusarium graminearum |
Crystallization (Comment) | Organism |
---|---|
sitting drop method. 2-5 mg/ml of enzyme mixed with equal volume of well solution. Crystals are soaked in oxygen-free Cu2+ solutions | Fusarium graminearum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | inhibits the formation of the thioether bond under anaerobic conditions (posttranslational modification) | Fusarium graminearum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Fusarium graminearum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68500 | - |
recombinant galactose oxidase without the pro sequence, SDS-PAGE | Fusarium graminearum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium graminearum | P0CS93 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | cleavage of a secretion signal sequence, copper-dependent cleavage of an N-terminal pro sequence | Fusarium graminearum |
side-chain modification | copper-dependent formation of a C228-Y272 thioether bond, generation of the Y272 radical | Fusarium graminearum |
Purification (Comment) | Organism |
---|---|
purification of recombinant galactose oxidase under strict copper-free conditions | Fusarium graminearum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + O2 | - |
Fusarium graminearum | D-galacto-hexodialdose + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
galactose oxidase | - |
Fusarium graminearum |