Cloned (Comment) | Organism |
---|---|
functional expression of wild-type and mutant enzymes in Escherichia coli strain BL21 Star (DE3) cytoplasm, best in strain BL21 Star (DE3) at 25°C, induction for 4-6 h is optimal | Fusarium graminearum |
Protein Variants | Comment | Organism |
---|---|---|
C383A | site-directed mutagenesis, the mutant shows 39% of wild-typ enzyme activity | Fusarium graminearum |
C383D | site-directed mutagenesis, the mutant shows 26% of wild-typ enzyme activity | Fusarium graminearum |
C383E | site-directed mutagenesis, the mutant shows 130% of wild-typ enzyme activity | Fusarium graminearum |
C383F | site-directed mutagenesis, the mutant shows 8% of wild-typ enzyme activity | Fusarium graminearum |
C383G | site-directed mutagenesis, the mutant shows 70% of wild-typ enzyme activity | Fusarium graminearum |
C383H | site-directed mutagenesis, the mutant shows 35% of wild-typ enzyme activity | Fusarium graminearum |
C383I | site-directed mutagenesis, the mutant shows 3% of wild-typ enzyme activity | Fusarium graminearum |
C383K | site-directed mutagenesis, the mutant shows 115% of wild-typ enzyme activity | Fusarium graminearum |
C383L | site-directed mutagenesis, the mutant shows 41% of wild-typ enzyme activity | Fusarium graminearum |
C383M | site-directed mutagenesis, the mutant shows 75% of wild-typ enzyme activity | Fusarium graminearum |
C383N | site-directed mutagenesis, the mutant shows 6% of wild-typ enzyme activity | Fusarium graminearum |
C383P | site-directed mutagenesis, the mutant shows 18% of wild-typ enzyme activity | Fusarium graminearum |
C383Q | site-directed mutagenesis, the mutant shows 13% of wild-typ enzyme activity | Fusarium graminearum |
C383R | site-directed mutagenesis, the mutant shows 0.2% of wild-typ enzyme activity | Fusarium graminearum |
C383S | site-directed mutagenesis, the mutant shows 160% of wild-typ enzyme activity | Fusarium graminearum |
C383T | site-directed mutagenesis, the mutant shows 32% of wild-typ enzyme activity | Fusarium graminearum |
C383V | site-directed mutagenesis, the mutant shows 4% of wild-typ enzyme activity | Fusarium graminearum |
C383W | site-directed mutagenesis, the mutant shows 0.001% of wild-typ enzyme activity | Fusarium graminearum |
C383Y | site-directed mutagenesis, the mutant cannt be isolated | Fusarium graminearum |
G195E | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Fusarium graminearum |
M70V | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Fusarium graminearum |
additional information | introduction of silent mutations within codons 2-7 of the mature enzyme coding sequence to enhance enzyme translation and have combined these with other expression-enhancing mutations | Fusarium graminearum |
N535D | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Fusarium graminearum |
V494A | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Fusarium graminearum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0021 | - |
D-galactose | pH 7.0, 25°C, mutant C383E | Fusarium graminearum | |
0.0084 | - |
D-galactose | pH 7.0, 25°C, mutant C383D | Fusarium graminearum | |
0.03 | - |
D-galactose | pH 7.0, 25°C, mutant C383H | Fusarium graminearum | |
0.033 | - |
D-galactose | pH 7.0, 25°C, mutant C383M | Fusarium graminearum | |
0.034 | - |
D-galactose | pH 7.0, 25°C, mutant C383S | Fusarium graminearum | |
0.048 | - |
D-galactose | pH 7.0, 25°C, mutant C383K | Fusarium graminearum | |
0.054 | - |
D-galactose | pH 7.0, 25°C, wild-type enzyme | Fusarium graminearum | |
0.055 | - |
D-galactose | pH 7.0, 25°C, mutant C383L | Fusarium graminearum | |
0.06 | - |
D-galactose | pH 7.0, 25°C, mutant C383W | Fusarium graminearum | |
0.064 | - |
D-galactose | pH 7.0, 25°C, mutant C383Q | Fusarium graminearum | |
0.083 | - |
D-galactose | pH 7.0, 25°C, mutant C383G | Fusarium graminearum | |
0.13 | - |
D-galactose | pH 7.0, 25°C, mutant C383F | Fusarium graminearum | |
0.14 | - |
D-galactose | pH 7.0, 25°C, mutant C383P | Fusarium graminearum | |
0.16 | - |
D-galactose | pH 7.0, 25°C, mutant C383A | Fusarium graminearum | |
0.29 | - |
D-galactose | pH 7.0, 25°C, mutant C383R | Fusarium graminearum | |
0.39 | - |
D-galactose | pH 7.0, 25°C, mutant C383N | Fusarium graminearum | |
0.43 | - |
D-galactose | pH 7.0, 25°C, mutant C383V | Fusarium graminearum | |
0.51 | - |
D-galactose | pH 7.0, 25°C, mutant C383I | Fusarium graminearum | |
0.53 | - |
D-galactose | pH 7.0, 25°C, mutant C383T | Fusarium graminearum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + O2 | Fusarium graminearum | - |
D-galacto-hexodialdose + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium graminearum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + O2 | - |
Fusarium graminearum | D-galacto-hexodialdose + H2O2 | - |
? | |
additional information | the enzyme shows broad primary alcohol substrate specificity | Fusarium graminearum | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Fusarium graminearum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
D-galactose | pH 7.0, 25°C, mutant C383W | Fusarium graminearum | |
8.8 | - |
D-galactose | pH 7.0, 25°C, mutant C383R | Fusarium graminearum | |
170 | - |
D-galactose | pH 7.0, 25°C, mutant C383Q | Fusarium graminearum | |
190 | - |
D-galactose | pH 7.0, 25°C, mutant C383F | Fusarium graminearum | |
210 | - |
D-galactose | pH 7.0, 25°C, mutant C383H | Fusarium graminearum | |
260 | - |
D-galactose | pH 7.0, 25°C, mutant C383I | Fusarium graminearum | |
360 | - |
D-galactose | pH 7.0, 25°C, mutant C383V | Fusarium graminearum | |
410 | - |
D-galactose | pH 7.0, 25°C, mutant C383N | Fusarium graminearum | |
440 | - |
D-galactose | pH 7.0, 25°C, mutant C383D | Fusarium graminearum | |
450 | - |
D-galactose | pH 7.0, 25°C, mutant C383L | Fusarium graminearum | |
490 | - |
D-galactose | pH 7.0, 25°C, mutant C383P | Fusarium graminearum | |
510 | - |
D-galactose | pH 7.0, 25°C, mutant C383M | Fusarium graminearum | |
550 | - |
D-galactose | pH 7.0, 25°C, mutant C383E | Fusarium graminearum | |
1100 | - |
D-galactose | pH 7.0, 25°C, wild-type enzyme | Fusarium graminearum | |
1100 | - |
D-galactose | pH 7.0, 25°C, mutant C383G | Fusarium graminearum | |
1100 | - |
D-galactose | pH 7.0, 25°C, mutant C383K | Fusarium graminearum | |
1100 | - |
D-galactose | pH 7.0, 25°C, mutant C383S | Fusarium graminearum | |
1200 | - |
D-galactose | pH 7.0, 25°C, mutant C383A | Fusarium graminearum | |
3400 | - |
D-galactose | pH 7.0, 25°C, mutant C383T | Fusarium graminearum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Fusarium graminearum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00019 | - |
D-galactose | pH 7.0, 25°C, mutant C383W | Fusarium graminearum | |
0.03 | - |
D-galactose | pH 7.0, 25°C, mutant C383R | Fusarium graminearum | |
0.51 | - |
D-galactose | pH 7.0, 25°C, mutant C383I | Fusarium graminearum | |
0.83 | - |
D-galactose | pH 7.0, 25°C, mutant C383V | Fusarium graminearum | |
1.1 | - |
D-galactose | pH 7.0, 25°C, mutant C383N | Fusarium graminearum | |
1.5 | - |
D-galactose | pH 7.0, 25°C, mutant C383F | Fusarium graminearum | |
2.6 | - |
D-galactose | pH 7.0, 25°C, mutant C383Q | Fusarium graminearum | |
3.5 | - |
D-galactose | pH 7.0, 25°C, mutant C383P | Fusarium graminearum | |
5.2 | - |
D-galactose | pH 7.0, 25°C, mutant C383D | Fusarium graminearum | |
6.4 | - |
D-galactose | pH 7.0, 25°C, mutant C383T | Fusarium graminearum | |
7 | - |
D-galactose | pH 7.0, 25°C, mutant C383H | Fusarium graminearum | |
7.7 | - |
D-galactose | pH 7.0, 25°C, mutant C383A | Fusarium graminearum | |
8.2 | - |
D-galactose | pH 7.0, 25°C, mutant C383L | Fusarium graminearum | |
14 | - |
D-galactose | pH 7.0, 25°C, mutant C383G | Fusarium graminearum | |
15 | - |
D-galactose | pH 7.0, 25°C, mutant C383M | Fusarium graminearum | |
20 | - |
D-galactose | pH 7.0, 25°C, wild-type enzyme | Fusarium graminearum | |
23 | - |
D-galactose | pH 7.0, 25°C, mutant C383K | Fusarium graminearum | |
26 | - |
D-galactose | pH 7.0, 25°C, mutant C383E | Fusarium graminearum | |
32 | - |
D-galactose | pH 7.0, 25°C, mutant C383S | Fusarium graminearum |