Crystallization (Comment) | Organism |
---|---|
mutant C383S | Fusarium sp. |
Protein Variants | Comment | Organism |
---|---|---|
C383/Y436H | decrease in Km-value, increase in kcat-value | Fusarium sp. |
C383A | slight decrease in Km-value, decrease in kcat-value | Fusarium sp. |
C383H/V494A | decrease in Km-value, decrease in kcat-value | Fusarium sp. |
C383S | decrease in Km-value | Fusarium sp. |
C383S/Y436A | decrease in Km-value, decrease in kcat-value of 1-methyl-alpha-D-galactose, increase in kcat-value of D-galactose | Fusarium sp. |
C383S/Y436H/V494A | decrease in Km-value, increase in kcat-value | Fusarium sp. |
V494A | slight decrease in Km-value, increase in kcat-value | Fusarium sp. |
Y436A | Km- and kcat-values similar to wild type | Fusarium sp. |
Y436A/V494A | decrease in Km-value, increase in kcat-value | Fusarium sp. |
Y436H | slight decrease in Km-value, decrease in kcat-value | Fusarium sp. |
Y436H/V494A | slight decrease in kcat-value of 1-methyl-alpha-D-galactose, slight increase in kcat-value of D-galactose | Fusarium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
D-galactose | mutant C383S, pH 7.0, 25°C | Fusarium sp. | |
16 | - |
1-methyl-alpha-D-galactopyranoside | mutant C383S, pH 7.0, 25°C | Fusarium sp. | |
16 | - |
D-galactose | mutant C383S/V494A, pH 7.0, 25°C | Fusarium sp. | |
17 | - |
1-methyl-alpha-D-galactopyranoside | mutant C383S/V494A, pH 7.0, 25°C | Fusarium sp. | |
17 | - |
D-galactose | mutant C383S/Y436A, pH 7.0, 25°C | Fusarium sp. | |
17 | - |
1-methyl-alpha-D-galactopyranoside | mutant C383S/Y436H, pH 7.0, 25°C | Fusarium sp. | |
18 | - |
1-methyl-alpha-D-galactopyranoside | mutant C383S/Y436H/V494A, pH 7.0, 25°C | Fusarium sp. | |
19 | - |
D-galactose | mutant C383S/Y436H/V494A, pH 7.0, 25°C | Fusarium sp. | |
22 | - |
D-galactose | mutant C383S/Y436H, pH 7.0, 25°C | Fusarium sp. | |
25 | - |
1-methyl-alpha-D-galactopyranoside | mutant C383S/Y436A, pH 7.0, 25°C | Fusarium sp. | |
30 | - |
D-galactose | mutant Y436A/V494A, pH 7.0, 25°C | Fusarium sp. | |
45 | - |
1-methyl-alpha-D-galactopyranoside | mutant Y436H/v494A, pH 7.0, 25°C | Fusarium sp. | |
46 | - |
1-methyl-alpha-D-galactopyranoside | mutant V494A, pH 7.0, 25°C | Fusarium sp. | |
46 | - |
1-methyl-alpha-D-galactopyranoside | mutant Y436A, pH 7.0, 25°C | Fusarium sp. | |
46 | - |
1-methyl-alpha-D-galactopyranoside | mutant Y436A/V494A, pH 7.0, 25°C | Fusarium sp. | |
47 | - |
1-methyl-alpha-D-galactopyranoside | mutant C383A, pH 7.0, 25°C | Fusarium sp. | |
53 | - |
D-galactose | mutant V494A, pH 7.0, 25°C | Fusarium sp. | |
53 | - |
D-galactose | mutant Y436H, pH 7.0, 25°C | Fusarium sp. | |
55 | - |
1-methyl-alpha-D-galactopyranoside | mutant Y436H, pH 7.0, 25°C | Fusarium sp. | |
56 | - |
1-methyl-alpha-D-galactopyranoside | wild type, pH 7.0, 25°C | Fusarium sp. | |
61 | - |
D-galactose | mutant Y436A, pH 7.0, 25°C | Fusarium sp. | |
62 | - |
D-galactose | mutant C383A, pH 7.0, 25°C | Fusarium sp. | |
68 | - |
D-galactose | mutant Y436H/v494A, pH 7.0, 25°C | Fusarium sp. | |
68 | - |
D-galactose | wild type, pH 7.0, 25°C | Fusarium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68527 | - |
x * 68527, electr0spray mass spectrometry | Fusarium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium sp. | - |
expression in Pichia pastoris | - |
Fusarium sp. NRRL 2903 | - |
expression in Pichia pastoris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-methyl-alpha-D-galactopyranoside + O2 | - |
Fusarium sp. | 1-O-methyl-alpha-D-galacto-hexodialdose + H2O2 | - |
? | |
1-methyl-alpha-D-galactopyranoside + O2 | - |
Fusarium sp. NRRL 2903 | 1-O-methyl-alpha-D-galacto-hexodialdose + H2O2 | - |
? | |
D-galactose + O2 | - |
Fusarium sp. | D-galacto-hexodialdose + H2O2 | - |
? | |
D-galactose + O2 | - |
Fusarium sp. NRRL 2903 | D-galacto-hexodialdose + H2O2 | - |
? | |
guar + O2 | - |
Fusarium sp. | ? + H2O2 | - |
? | |
guar + O2 | - |
Fusarium sp. NRRL 2903 | ? + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 68527, electr0spray mass spectrometry | Fusarium sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.7 | - |
guar | mutant C383A, pH 7.0, 25°C | Fusarium sp. | |
26.3 | - |
guar | mutant Y436A, pH 7.0, 25°C | Fusarium sp. | |
26.3 | - |
guar | mutant Y436H/v494A, pH 7.0, 25°C | Fusarium sp. | |
29.7 | - |
guar | mutant Y436A/V494A, pH 7.0, 25°C | Fusarium sp. | |
30.4 | - |
guar | mutant Y436H, pH 7.0, 25°C | Fusarium sp. | |
32 | - |
guar | mutant V494A, pH 7.0, 25°C | Fusarium sp. | |
37.7 | - |
guar | mutant C383S/Y436H, pH 7.0, 25°C | Fusarium sp. | |
41.1 | - |
guar | mutant C383S, pH 7.0, 25°C | Fusarium sp. | |
41.1 | - |
guar | mutant C383S/Y436A, pH 7.0, 25°C | Fusarium sp. | |
42.8 | - |
guar | wild type, pH 7.0, 25°C | Fusarium sp. | |
44.5 | - |
guar | mutant C383S/Y436H/V494A, pH 7.0, 25°C | Fusarium sp. | |
58 | - |
guar | mutant C383S/V494A, pH 7.0, 25°C | Fusarium sp. | |
457 | - |
1-methyl-alpha-D-galactopyranoside | mutant C383A, pH 7.0, 25°C | Fusarium sp. | |
639 | - |
D-galactose | mutant C383A, pH 7.0, 25°C | Fusarium sp. | |
756 | - |
D-galactose | mutant Y436H, pH 7.0, 25°C | Fusarium sp. | |
811 | - |
1-methyl-alpha-D-galactopyranoside | mutant C383S/Y436A, pH 7.0, 25°C | Fusarium sp. | |
834 | - |
D-galactose | mutant C383S, pH 7.0, 25°C | Fusarium sp. | |
839 | - |
1-methyl-alpha-D-galactopyranoside | mutant C383S, pH 7.0, 25°C | Fusarium sp. | |
851 | - |
1-methyl-alpha-D-galactopyranoside | mutant Y436H/v494A, pH 7.0, 25°C | Fusarium sp. | |
915 | - |
D-galactose | mutant C383S/V494A, pH 7.0, 25°C | Fusarium sp. | |
995 | - |
1-methyl-alpha-D-galactopyranoside | wild type, pH 7.0, 25°C | Fusarium sp. | |
1039 | - |
1-methyl-alpha-D-galactopyranoside | mutant Y436A, pH 7.0, 25°C | Fusarium sp. | |
1067 | - |
1-methyl-alpha-D-galactopyranoside | mutant C383S/Y436H/V494A, pH 7.0, 25°C | Fusarium sp. | |
1090 | - |
D-galactose | wild type, pH 7.0, 25°C | Fusarium sp. | |
1091 | - |
1-methyl-alpha-D-galactopyranoside | mutant C383S/Y436H, pH 7.0, 25°C | Fusarium sp. | |
1100 | - |
D-galactose | mutant Y436A, pH 7.0, 25°C | Fusarium sp. | |
1119 | - |
D-galactose | mutant V494A, pH 7.0, 25°C | Fusarium sp. | |
1181 | - |
D-galactose | mutant Y436H/v494A, pH 7.0, 25°C | Fusarium sp. | |
1266 | - |
D-galactose | mutant C383S/Y436A, pH 7.0, 25°C | Fusarium sp. | |
1267 | - |
1-methyl-alpha-D-galactopyranoside | mutant Y436H, pH 7.0, 25°C | Fusarium sp. | |
1301 | - |
D-galactose | mutant C383S/Y436H/V494A, pH 7.0, 25°C | Fusarium sp. | |
1398 | - |
D-galactose | mutant Y436A/V494A, pH 7.0, 25°C | Fusarium sp. | |
1444 | - |
1-methyl-alpha-D-galactopyranoside | mutant V494A, pH 7.0, 25°C | Fusarium sp. | |
1495 | - |
1-methyl-alpha-D-galactopyranoside | mutant C383S/V494A, pH 7.0, 25°C | Fusarium sp. | |
1548 | - |
1-methyl-alpha-D-galactopyranoside | mutant Y436A/V494A, pH 7.0, 25°C | Fusarium sp. | |
1871 | - |
D-galactose | mutant C383S/Y436H, pH 7.0, 25°C | Fusarium sp. |