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Literature summary for 1.1.3.9 extracted from

  • Wilkinson, D.; Akumanyi, N.; Hurtado-Guerrero, R.; Dawkes, H.; Knowles, P.F.; Phillips, S.E.; McPherson, M.J.
    Structural and kinetic studies of a series of mutants of galactose oxidase identified by directed evolution (2004), Protein Eng. Des. Sel., 17, 141-148.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant C383S Fusarium sp.

Protein Variants

Protein Variants Comment Organism
C383/Y436H decrease in Km-value, increase in kcat-value Fusarium sp.
C383A slight decrease in Km-value, decrease in kcat-value Fusarium sp.
C383H/V494A decrease in Km-value, decrease in kcat-value Fusarium sp.
C383S decrease in Km-value Fusarium sp.
C383S/Y436A decrease in Km-value, decrease in kcat-value of 1-methyl-alpha-D-galactose, increase in kcat-value of D-galactose Fusarium sp.
C383S/Y436H/V494A decrease in Km-value, increase in kcat-value Fusarium sp.
V494A slight decrease in Km-value, increase in kcat-value Fusarium sp.
Y436A Km- and kcat-values similar to wild type Fusarium sp.
Y436A/V494A decrease in Km-value, increase in kcat-value Fusarium sp.
Y436H slight decrease in Km-value, decrease in kcat-value Fusarium sp.
Y436H/V494A slight decrease in kcat-value of 1-methyl-alpha-D-galactose, slight increase in kcat-value of D-galactose Fusarium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
15
-
D-galactose mutant C383S, pH 7.0, 25°C Fusarium sp.
16
-
1-methyl-alpha-D-galactopyranoside mutant C383S, pH 7.0, 25°C Fusarium sp.
16
-
D-galactose mutant C383S/V494A, pH 7.0, 25°C Fusarium sp.
17
-
1-methyl-alpha-D-galactopyranoside mutant C383S/V494A, pH 7.0, 25°C Fusarium sp.
17
-
D-galactose mutant C383S/Y436A, pH 7.0, 25°C Fusarium sp.
17
-
1-methyl-alpha-D-galactopyranoside mutant C383S/Y436H, pH 7.0, 25°C Fusarium sp.
18
-
1-methyl-alpha-D-galactopyranoside mutant C383S/Y436H/V494A, pH 7.0, 25°C Fusarium sp.
19
-
D-galactose mutant C383S/Y436H/V494A, pH 7.0, 25°C Fusarium sp.
22
-
D-galactose mutant C383S/Y436H, pH 7.0, 25°C Fusarium sp.
25
-
1-methyl-alpha-D-galactopyranoside mutant C383S/Y436A, pH 7.0, 25°C Fusarium sp.
30
-
D-galactose mutant Y436A/V494A, pH 7.0, 25°C Fusarium sp.
45
-
1-methyl-alpha-D-galactopyranoside mutant Y436H/v494A, pH 7.0, 25°C Fusarium sp.
46
-
1-methyl-alpha-D-galactopyranoside mutant V494A, pH 7.0, 25°C Fusarium sp.
46
-
1-methyl-alpha-D-galactopyranoside mutant Y436A, pH 7.0, 25°C Fusarium sp.
46
-
1-methyl-alpha-D-galactopyranoside mutant Y436A/V494A, pH 7.0, 25°C Fusarium sp.
47
-
1-methyl-alpha-D-galactopyranoside mutant C383A, pH 7.0, 25°C Fusarium sp.
53
-
D-galactose mutant V494A, pH 7.0, 25°C Fusarium sp.
53
-
D-galactose mutant Y436H, pH 7.0, 25°C Fusarium sp.
55
-
1-methyl-alpha-D-galactopyranoside mutant Y436H, pH 7.0, 25°C Fusarium sp.
56
-
1-methyl-alpha-D-galactopyranoside wild type, pH 7.0, 25°C Fusarium sp.
61
-
D-galactose mutant Y436A, pH 7.0, 25°C Fusarium sp.
62
-
D-galactose mutant C383A, pH 7.0, 25°C Fusarium sp.
68
-
D-galactose mutant Y436H/v494A, pH 7.0, 25°C Fusarium sp.
68
-
D-galactose wild type, pH 7.0, 25°C Fusarium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68527
-
x * 68527, electr0spray mass spectrometry Fusarium sp.

Organism

Organism UniProt Comment Textmining
Fusarium sp.
-
expression in Pichia pastoris
-
Fusarium sp. NRRL 2903
-
expression in Pichia pastoris
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-methyl-alpha-D-galactopyranoside + O2
-
Fusarium sp. 1-O-methyl-alpha-D-galacto-hexodialdose + H2O2
-
?
1-methyl-alpha-D-galactopyranoside + O2
-
Fusarium sp. NRRL 2903 1-O-methyl-alpha-D-galacto-hexodialdose + H2O2
-
?
D-galactose + O2
-
Fusarium sp. D-galacto-hexodialdose + H2O2
-
?
D-galactose + O2
-
Fusarium sp. NRRL 2903 D-galacto-hexodialdose + H2O2
-
?
guar + O2
-
Fusarium sp. ? + H2O2
-
?
guar + O2
-
Fusarium sp. NRRL 2903 ? + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 68527, electr0spray mass spectrometry Fusarium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.7
-
guar mutant C383A, pH 7.0, 25°C Fusarium sp.
26.3
-
guar mutant Y436A, pH 7.0, 25°C Fusarium sp.
26.3
-
guar mutant Y436H/v494A, pH 7.0, 25°C Fusarium sp.
29.7
-
guar mutant Y436A/V494A, pH 7.0, 25°C Fusarium sp.
30.4
-
guar mutant Y436H, pH 7.0, 25°C Fusarium sp.
32
-
guar mutant V494A, pH 7.0, 25°C Fusarium sp.
37.7
-
guar mutant C383S/Y436H, pH 7.0, 25°C Fusarium sp.
41.1
-
guar mutant C383S, pH 7.0, 25°C Fusarium sp.
41.1
-
guar mutant C383S/Y436A, pH 7.0, 25°C Fusarium sp.
42.8
-
guar wild type, pH 7.0, 25°C Fusarium sp.
44.5
-
guar mutant C383S/Y436H/V494A, pH 7.0, 25°C Fusarium sp.
58
-
guar mutant C383S/V494A, pH 7.0, 25°C Fusarium sp.
457
-
1-methyl-alpha-D-galactopyranoside mutant C383A, pH 7.0, 25°C Fusarium sp.
639
-
D-galactose mutant C383A, pH 7.0, 25°C Fusarium sp.
756
-
D-galactose mutant Y436H, pH 7.0, 25°C Fusarium sp.
811
-
1-methyl-alpha-D-galactopyranoside mutant C383S/Y436A, pH 7.0, 25°C Fusarium sp.
834
-
D-galactose mutant C383S, pH 7.0, 25°C Fusarium sp.
839
-
1-methyl-alpha-D-galactopyranoside mutant C383S, pH 7.0, 25°C Fusarium sp.
851
-
1-methyl-alpha-D-galactopyranoside mutant Y436H/v494A, pH 7.0, 25°C Fusarium sp.
915
-
D-galactose mutant C383S/V494A, pH 7.0, 25°C Fusarium sp.
995
-
1-methyl-alpha-D-galactopyranoside wild type, pH 7.0, 25°C Fusarium sp.
1039
-
1-methyl-alpha-D-galactopyranoside mutant Y436A, pH 7.0, 25°C Fusarium sp.
1067
-
1-methyl-alpha-D-galactopyranoside mutant C383S/Y436H/V494A, pH 7.0, 25°C Fusarium sp.
1090
-
D-galactose wild type, pH 7.0, 25°C Fusarium sp.
1091
-
1-methyl-alpha-D-galactopyranoside mutant C383S/Y436H, pH 7.0, 25°C Fusarium sp.
1100
-
D-galactose mutant Y436A, pH 7.0, 25°C Fusarium sp.
1119
-
D-galactose mutant V494A, pH 7.0, 25°C Fusarium sp.
1181
-
D-galactose mutant Y436H/v494A, pH 7.0, 25°C Fusarium sp.
1266
-
D-galactose mutant C383S/Y436A, pH 7.0, 25°C Fusarium sp.
1267
-
1-methyl-alpha-D-galactopyranoside mutant Y436H, pH 7.0, 25°C Fusarium sp.
1301
-
D-galactose mutant C383S/Y436H/V494A, pH 7.0, 25°C Fusarium sp.
1398
-
D-galactose mutant Y436A/V494A, pH 7.0, 25°C Fusarium sp.
1444
-
1-methyl-alpha-D-galactopyranoside mutant V494A, pH 7.0, 25°C Fusarium sp.
1495
-
1-methyl-alpha-D-galactopyranoside mutant C383S/V494A, pH 7.0, 25°C Fusarium sp.
1548
-
1-methyl-alpha-D-galactopyranoside mutant Y436A/V494A, pH 7.0, 25°C Fusarium sp.
1871
-
D-galactose mutant C383S/Y436H, pH 7.0, 25°C Fusarium sp.