Activating Compound | Comment | Organism | Structure |
---|---|---|---|
chloroiridate | marked increase of activity | Hypomyces rosellus | |
EDTA | up to 7fold activation | Hypomyces rosellus | |
ferricyanide | marked activation at 1 mM | Hypomyces rosellus | |
superoxide | stimulation of O2 uptake | Hypomyces rosellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ferricyanide | inhibition at 0.001 mM, activation at 1 mM | Hypomyces rosellus | |
H2O2 | irreversible inactivation, only during active catalysis | Hypomyces rosellus | |
Superoxide dismutase | 50% inhibition at 50 pM | Hypomyces rosellus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Hypomyces rosellus | - |
- |
extracellular | - |
Polyporus circinatus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu3+ | first evidence of oxidation by the Cu(III) enzyme with concomitant formation of a Cu(I) enzyme | Hypomyces rosellus | |
Mn2+ | marked increase of actvitiy at micromolar concentrations | Hypomyces rosellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactosylpyranoside + O2 | Hypomyces rosellus | - |
D-galacto-hexodialdose + H2O2 | - |
? | |
D-galactosylpyranoside + O2 | Polyporus circinatus | - |
D-galacto-hexodialdose + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hypomyces rosellus | - |
- |
- |
Polyporus circinatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Hypomyces rosellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Hypomyces rosellus | - |
mycelium | - |
Polyporus circinatus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
468 | - |
- |
Hypomyces rosellus |
Storage Stability | Organism |
---|---|
4°C, 0.01 M phosphate buffer, pH 7.0, 0.1 mg/ml protein, 30% decrease of activity in 1 year | Hypomyces rosellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-O-methyl-alpha-D-galactosylpyranoside + O2 | - |
Hypomyces rosellus | 1-O-methyl-alpha-D-galacto-hexodialdose + H2O2 | - |
? | |
1-O-methyl-alpha-D-galactosylpyranoside + O2 | - |
Hypomyces rosellus | 1-O-methyl-alpha-D-galacto-hexodialdose + H2O2 | - |
ir | |
1-O-methyl-alpha-D-galactosylpyranoside + O2 | - |
Polyporus circinatus | 1-O-methyl-alpha-D-galacto-hexodialdose + H2O2 | - |
? | |
D-galactopyranose + ferricyanide | ferricyanide poorly replaces O2 as electron acceptor | Hypomyces rosellus | D-galacto-hexodialdose + ferrocyanide | - |
? | |
D-galactosylpyranoside + O2 | - |
Hypomyces rosellus | D-galacto-hexodialdose + H2O2 | - |
? | |
D-galactosylpyranoside + O2 | - |
Polyporus circinatus | D-galacto-hexodialdose + H2O2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20 | - |
1-O-methyl-alpha-D-galactopyranoside | - |
Hypomyces rosellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Hypomyces rosellus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
slow inactivation below | Hypomyces rosellus |
8 | - |
slow inactivation above | Hypomyces rosellus |