Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.7.4.25: (d)CMP kinase

This is an abbreviated version!
For detailed information about (d)CMP kinase, go to the full flat file.

Word Map on EC 2.7.4.25

Reaction

ATP
+
(d)CMP
=
ADP
 +
(d)CDP

Synonyms

ATP:CMP phosphotransferase, bacterial cytidylate kinase, CMK, CMP kinase, CMPK, cytidine 5’-monophosphate kinase, cytidine monophosphate kinase, cytidylate kinase, dCMP kinase, deoxycytidine monophosphate kinase, deoxycytidine monophosphokinase, deoxycytidylate kinase, More, P25 protein, prokaryotic cytidylate kinase, SP1603, UCK, UMP-CMP kinase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.25 (d)CMP kinase

Crystallization

Crystallization on EC 2.7.4.25 - (d)CMP kinase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme in complex with CDP
-
hanging drop vapor diffusion technique
-
in complex with substrates CMP, dCMP, ara-CMP and 2’,3’-dideoxy-CMP, hanging drop vapor diffusion method, using ammonium sulfate in a 50 mM Tris-HCl buffer (pH 7.4), at 20°C
of the R188M mutant alone or with dCMP
purified recombinant mutant R188M free or in complex with CMP, X-ray diffraction structure determination and analysis at 1.9 A and 2.8 A resolution, respectively
hanging drop vapor diffusion method, using 0.4 M ammonium sulfate in 50 mM Tris-HCl buffer (pH 7.4), at 20°C
mutant enzyme R188M is crystallized either alone or in complex with dCMP, hanging drop vapor diffusion method, using ammonium sulfate as a precipitant (1.3 M in the case of enzyme alone, and 1.7 M for the R188M-dCMP complex)