Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.4.25 extracted from

  • Alexandre, J.A.; Roy, B.; Topalis, D.; Pochet, S.; Perigaud, C.; Deville-Bonne, D.
    Enantioselectivity of human AMP, dTMP and UMP-CMP kinases (2007), Nucleic Acids Res., 35, 4895-4904.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.25

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
of the R188M mutant alone or with dCMP Escherichia coli

Protein Variants

Protein Variants Comment Organism
D132A site directed mutagenesis Escherichia coli
D132H site directed mutagenesis Escherichia coli
D132N site directed mutagenesis Escherichia coli
D132S site directed mutagenesis Escherichia coli
R110M site directed mutagenesis Escherichia coli
R188M site directed mutagenesis Escherichia coli
S36A site directed mutagenesis Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 CMP wild type Escherichia coli
0.038
-
 CMP D132S mutant Escherichia coli
0.055
-
 dCMP D132H mutant Escherichia coli
0.08
-
 dCMP D132N mutant Escherichia coli
0.09
-
 dCMP D132S mutant Escherichia coli
0.094
-
 dCMP wild type Escherichia coli
0.77
-
 dCMP R110M mutant Escherichia coli
0.93
-
 UMP wild type Escherichia coli
1
-
 CMP R110M mutant Escherichia coli
1.3
-
 CMP D132H mutant Escherichia coli
1.8
-
 dCMP D132A mutant Escherichia coli
1.9
-
 UMP S36A mutant Escherichia coli
2.5
-
 CMP S36A mutant Escherichia coli
2.6
-
 CMP D132N mutant Escherichia coli
2.9
-
 CMP D132A mutant Escherichia coli
3.5
-
 dCMP S36A mutant Escherichia coli
3.9
-
 UMP D132H mutant Escherichia coli
4.5
-
 UMP D132N mutant Escherichia coli
7.3
-
 dCMP R110M mutant Escherichia coli
7.9
-
 UMP D132A mutant Escherichia coli
8
-
 UMP D132S mutant Escherichia coli
11.3
-
 UMP R110M mutant Escherichia coli
20.2
-
 CMP R110M mutant Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A6I0
-
-

Purification (Commentary)

Purification (Comment) Organism
of the recombinant proteins Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + CMP
-
 Escherichia coli ADP + CDP
-
 ?
ATP + dCMP
-
 Escherichia coli ADP + dCDP
-
 ?
ATP + UMP
-
 Escherichia coli ADP + UDP
-
 ?

Synonyms

Synonyms Comment Organism
CMP kinase
-
 Escherichia coli
cytidylate kinase
-
 Escherichia coli
UMP-CMP kinase
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
43
-
 mutant D132A Escherichia coli
45
-
 mutant R110M Escherichia coli
47
-
 mutant D132N Escherichia coli
48
-
 mutant D132S, mutant D132H Escherichia coli
51
-
 mutant R188M Escherichia coli
52
-
 wild type, mutant S36A Escherichia coli