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Literature summary for 2.7.4.25 extracted from

  • Bucurenci, N.; Sakamoto, H.; Briozzo, P.; Palibroda, N.; Serina, L.; Sarfati, R.S.; Labesse, G.; Briand, G.; Danchin, A.; Barzu, O.; Gilles, A.M.
    CMP kinase from Escherichia coli is structurally related to other nucleoside monophosphate kinases (1996), J. Biol. Chem., 271, 2856-2862.
    View publication on PubMed
Search for more literature for 2.7.4.25

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli K-12

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.4 M ammonium sulfate in 50 mM Tris-HCl buffer (pH 7.4), at 20°C Escherichia coli K-12
hanging drop vapor diffusion technique Escherichia coli

Protein Variants

Protein Variants Comment Organism
V164E substitution of Val164 by a Glu residue apparently does not affect the catalytic properties of Escherichia coli CMP kinase Escherichia coli K-12

Inhibitors

Inhibitors Comment Organism Structure
additional information TMP, Ant-dCMP, 5-methyl-CMP, AMP, dAMP, GMP, and dGMP are no inhibitors of the enzyme Escherichia coli K-12

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 CMP reaction with ATP Escherichia coli
0.035
-
 CMP in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C Escherichia coli K-12
0.038
-
 ATP pH 7.4, reaction with CMP Escherichia coli
0.038
-
 ATP in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C Escherichia coli K-12
0.087
-
 dATP pH 7.4, reaction with CMP Escherichia coli
0.087
-
 dATP in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C Escherichia coli K-12
0.094
-
 dGMP reaction with ATP Escherichia coli
0.094
-
 dCMP in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C Escherichia coli K-12
0.36
-
 araCMP reaction with ATP Escherichia coli
0.36
-
 1-beta-D-arabinofuranosylcytosine 5'-phosphate in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C Escherichia coli K-12
0.64
-
 GTP reaction with CMP Escherichia coli
0.64
-
 GTP in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C Escherichia coli K-12
0.87
-
 ITP in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C Escherichia coli K-12
0.93
-
 UMP reaction with ATP Escherichia coli
0.93
-
 UMP in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C Escherichia coli K-12
1.46
-
 dUMP in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C Escherichia coli K-12

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24620
-
 electrospray ionization mass spectrometry Escherichia coli K-12

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli K-12 P0A6I0 strain NM554
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli
blue Sepharose column chromatography and Ultrogel AcA54 gel filtration Escherichia coli K-12

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
 Escherichia coli K-12 ADP + 1-beta-D-arabinofuranosylcytosine 5'-diphosphate
-
 ?
ATP + 2-thiouridine 5'-monophosphate 2-thiouridine 5'-monophosphate is a poor substrate Escherichia coli K-12 ADP + 2-thiouridine 5'-diphosphate
-
 ?
ATP + ara-CMP
-
 Escherichia coli ADP + ara-CDP
-
 ?
ATP + CMP
-
 Escherichia coli ADP + CDP
-
 ?
ATP + CMP
-
 Escherichia coli K-12 ADP + CDP
-
 ?
ATP + dCMP
-
 Escherichia coli ADP + dCDP
-
 ?
ATP + dCMP
-
 Escherichia coli K-12 ADP + dCDP
-
 ?
ATP + dGMP
-
 Escherichia coli ADP + dGDP
-
 ?
ATP + dUMP dUMP is a poor substrate Escherichia coli K-12 ADP + dUDP
-
 ?
ATP + UMP
-
 Escherichia coli ADP + UDP
-
 ?
ATP + UMP UMP is a poor substrate Escherichia coli K-12 ADP + UDP
-
 ?
CTP + CMP the activity with CTP is still measurable but less than 0.05% of that with ATP Escherichia coli K-12 CDP + CDP
-
 ?
dATP + CMP
-
 Escherichia coli dADP + CDP
-
 ?
dATP + CMP
-
 Escherichia coli K-12 dADP + CDP
-
 ?
dCTP + CMP the activity with dCTP is still measurable but less than 0.05% of that with ATP Escherichia coli K-12 dCDP + CDP
-
 ?
dUTP + CMP the activity with dUTP is still measurable but less than 0.05% of that with ATP Escherichia coli K-12 dUDP + CDP
-
 ?
GTP + CMP
-
 Escherichia coli GDP + CDP
-
 ?
GTP + CMP
-
 Escherichia coli K-12 GDP + CDP
-
 ?
ITP + CMP ITP is a poor substrate Escherichia coli K-12 IDP + CDP
-
 ?
additional information TMP, Ant-dCMP, 5-methyl-CMP, AMP, dAMP, GMP, and dGMP are no substrates Escherichia coli K-12 ?
-
 ?
UTP + CMP the activity with UTP is still measurable but less than 0.05% of that with ATP Escherichia coli K-12 UDP + CDP
-
 ?

Subunits

Subunits Comment Organism
monomer 2 * 000, SDS-PAGE Escherichia coli K-12

Synonyms

Synonyms Comment Organism
CMP kinase
-
 Escherichia coli K-12

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
52
-
 CMP kinase is half-inactivated at 52°C Escherichia coli K-12

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli K-12