2.7.4.25: (d)CMP kinase

This is an abbreviated version!
For detailed information about (d)CMP kinase, go to the full flat file.

Reaction

Synonyms

ATP:CMP phosphotransferase, bacterial cytidylate kinase, CMK, CMP kinase, CMPK, cytidine 5�-monophosphate kinase, cytidine monophosphate kinase, cytidylate kinase, dCMP kinase, deoxycytidine monophosphate kinase, deoxycytidine monophosphokinase, deoxycytidylate kinase, More, P25 protein, prokaryotic cytidylate kinase, SP1603, UCK, UMP-CMP kinase

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.4 Phosphotransferases with a phosphate group as acceptor

                2.7.4.25 (d)CMP kinase

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Results	in table
41245	AA Sequence
1	Application
12	Cloned(Commentary)
10	Cofactor
7	Crystallization (Commentary)
5	General Information
1	General Stability
1	Inhibitors
38	kcat/KM [mM/s]
98	KM Value [mM]
5	Metals/Ions
7	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
210	Organism
4	Pathway
9	PDB ID
25	Protein Variants
8	Purification (Commentary)
1	Reaction
3	Reaction Type
19	Reference
4	Specific Activity [micromol/min/mg]
2	Storage Stability
76	Substrates and Products (Substrate)
5	Subunits
44	Synonyms
1	Systematic Name
15	Temperature Stability [°C]
45	Turnover Number [1/s]

KM Value

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KM Value on EC 2.7.4.25 - (d)CMP kinase

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.36

1-beta-D-arabinofuranosylcytosine 5'-phosphate

Escherichia coli K-12

P0A6I0

in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

645154

0.15 - 0.65

2',3'-dideoxy-CMP

4 entries

0.37 - 1

ara-CMP

5 entries

0.36 - 0.37

araCMP

3 entries

0.038 - 0.12

ATP

3 entries

0.035 - 20.2

CMP

27 entries

0.087

dATP

2 entries

0.0394 - 7.3

dCMP

26 entries

0.094

dGMP

Escherichia coli

-

reaction with ATP

645154

1.46

dUMP

Escherichia coli K-12

P0A6I0

in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

645154

0.64

GTP

2 entries

0.87

ITP

Escherichia coli K-12

P0A6I0

in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

645154

0.93 - 13.85

UMP

21 entries

additional information

additional information

Escherichia coli

P0A6I0

kinetics and substrate specificities of wild-type and mutant enzymes, overview

692282

-

0.15

2',3'-dideoxy-CMP

Escherichia coli

A0A140N8S7

mutant enzyme D185A, at 30°C and pH 7.4

712736

0.46

2',3'-dideoxy-CMP

Escherichia coli

A0A140N8S7

wild type enzyme, at 30°C and pH 7.4

712736

0.54

2',3'-dideoxy-CMP

Escherichia coli

A0A140N8S7

mutant enzyme R181M, at 30°C and pH 7.4

712736

0.65

2',3'-dideoxy-CMP

Escherichia coli

A0A140N8S7

mutant enzyme S101A, at 30°C and pH 7.4

712736

0.37

ara-CMP

Bacillus subtilis

-

at 30°C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH

645159

0.47

ara-CMP

Escherichia coli

A0A140N8S7

mutant enzyme S101A, at 30°C and pH 7.4

712736

0.53

ara-CMP

Escherichia coli

A0A140N8S7

wild type enzyme, at 30°C and pH 7.4

712736

0.79

ara-CMP

Escherichia coli

A0A140N8S7

mutant enzyme R181M, at 30°C and pH 7.4

712736

1

ara-CMP

Escherichia coli

A0A140N8S7

mutant enzyme D185A, at 30°C and pH 7.4

712736

0.36

araCMP

Escherichia coli

-

pH 7.4, 30°C

645159

0.36

araCMP

Escherichia coli

-

reaction with ATP

645154

0.37

araCMP

Bacillus subtilis

-

pH 7.4, 30°C

645159

0.038

ATP

Escherichia coli

-

pH 7.4, reaction with CMP

645154

0.038

ATP

Escherichia coli K-12

P0A6I0

in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

645154

0.12

ATP

Bacillus subtilis

-

at 30°C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH

645159

0.035

CMP

Escherichia coli

P0A6I0

wild-type enzyme

692282

0.035

CMP

Escherichia coli

P0A6I0

wild type

676227

0.035

CMP

Escherichia coli

-

pH 7.4, 30°C

645159

0.035

CMP

Escherichia coli

-

reaction with ATP

645154

0.035

CMP

Escherichia coli K-12

P0A6I0

in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

645154

0.035

CMP

Escherichia coli

A0A140N8S7

wild type enzyme, at 30°C and pH 7.4

712736

0.035

CMP

Escherichia coli K-12

P0A6I0

wild type enzyme, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.038

CMP

Escherichia coli

P0A6I0

D132S mutant

676227

0.038

CMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132S, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.04

CMP

Bacillus subtilis

-

pH 7.4, 30°C

645159

0.04

CMP

Bacillus subtilis

-

at 30°C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH

645159

0.08

CMP

Escherichia coli

A0A140N8S7

mutant enzyme S101A, at 30°C and pH 7.4

712736

0.12

CMP

Mycobacterium tuberculosis

-

pH and temperature not specified in the publication

712276

0.19

CMP

Escherichia coli

A0A140N8S7

mutant enzyme R181M, at 30°C and pH 7.4

712736

0.47

CMP

Escherichia coli

A0A140N8S7

mutant enzyme D185A, at 30°C and pH 7.4

712736

1

CMP

Escherichia coli

P0A6I0

R110M mutant

676227

1

CMP

Escherichia coli K-12

P0A6I0

mutant enzyme R188M, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

1.3

CMP

Escherichia coli

P0A6I0

D132H mutant

676227

1.3

CMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132H, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

2.5

CMP

Escherichia coli

P0A6I0

S36A mutant

676227

2.5

CMP

Escherichia coli K-12

P0A6I0

mutant enzyme S36A, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

2.6

CMP

Escherichia coli

P0A6I0

D132N mutant

676227

2.6

CMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132N, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

2.9

CMP

Escherichia coli

P0A6I0

D132A mutant

676227

2.9

CMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132A, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

20.2

CMP

Escherichia coli

P0A6I0

R110M mutant

676227

20.2

CMP

Escherichia coli K-12

P0A6I0

mutant enzyme R110M, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.087

dATP

Escherichia coli

-

pH 7.4, reaction with CMP

645154

0.087

dATP

Escherichia coli K-12

P0A6I0

in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

645154

0.0394

dCMP

Escherichia coli K-12

P0A6I0

wild type enzyme, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.055

dCMP

Escherichia coli

P0A6I0

D132H mutant

676227

0.055

dCMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132H, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.08

dCMP

Escherichia coli

P0A6I0

D132N mutant

676227

0.08

dCMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132N, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.09

dCMP

Escherichia coli

P0A6I0

D132S mutant

676227

0.09

dCMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132S, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.094

dCMP

Escherichia coli

P0A6I0

wild-type enzyme

692282

0.094

dCMP

Escherichia coli

P0A6I0

wild type

676227

0.094

dCMP

Escherichia coli

-

pH 7.4, 30°C

645159

0.094

dCMP

Escherichia coli K-12

P0A6I0

in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

645154

0.094

dCMP

Escherichia coli

A0A140N8S7

wild type enzyme, at 30°C and pH 7.4

712736

0.165

dCMP

Mycobacterium tuberculosis

-

pH and temperature not specified in the publication

712276

0.19

dCMP

Escherichia coli

A0A140N8S7

mutant enzyme S101A, at 30°C and pH 7.4

712736

0.24

dCMP

Escherichia coli

A0A140N8S7

mutant enzyme D185A, at 30°C and pH 7.4

712736

0.24

dCMP

Escherichia coli

A0A140N8S7

mutant enzyme R181M, at 30°C and pH 7.4

712736

0.33

dCMP

Bacillus subtilis

-

pH 7.4, 30°C

645159

0.33

dCMP

Bacillus subtilis

-

at 30°C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH

645159

0.77

dCMP

Escherichia coli

P0A6I0

R110M mutant

676227

0.77

dCMP

Escherichia coli K-12

P0A6I0

mutant enzyme R188M, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

1.8

dCMP

Escherichia coli

P0A6I0

D132A mutant

676227

1.8

dCMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132A, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

3.5

dCMP

Escherichia coli

P0A6I0

S36A mutant

676227

3.5

dCMP

Escherichia coli K-12

P0A6I0

mutant enzyme S36A, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

7.3

dCMP

Escherichia coli

P0A6I0

R110M mutant

676227

7.3

dCMP

Escherichia coli K-12

P0A6I0

mutant enzyme R110M, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.64

GTP

Escherichia coli

-

reaction with CMP

645154

0.64

GTP

Escherichia coli K-12

P0A6I0

in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

645154

0.93

UMP

Escherichia coli

P0A6I0

wild-type enzyme

692282

0.93

UMP

Escherichia coli

P0A6I0

wild type

676227

0.93

UMP

Escherichia coli

-

pH 7.4, 30°C

645159

0.93

UMP

Escherichia coli

-

reaction with ATP

645154

0.93

UMP

Escherichia coli K-12

P0A6I0

in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

645154

0.93

UMP

Escherichia coli K-12

P0A6I0

wild type enzyme, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

1.9

UMP

Escherichia coli

P0A6I0

S36A mutant

676227

1.9

UMP

Escherichia coli K-12

P0A6I0

mutant enzyme S36A, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

3.6

UMP

Bacillus subtilis

-

pH 7.4, 30°C

645159

3.6

UMP

Bacillus subtilis

-

at 30°C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH

645159

3.9

UMP

Escherichia coli

P0A6I0

D132H mutant

676227

3.9

UMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132H, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

4.5

UMP

Escherichia coli

P0A6I0

D132N mutant

676227

5.4

UMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132N, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

7.9

UMP

Escherichia coli

P0A6I0

D132A mutant

676227

7.9

UMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132A, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

8

UMP

Escherichia coli

P0A6I0

D132S mutant

676227

8

UMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132S, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

11.3

UMP

Escherichia coli

P0A6I0

R110M mutant

676227

11.3

UMP

Escherichia coli K-12

P0A6I0

mutant enzyme R110M, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

13.85

UMP

Mycobacterium tuberculosis

-

pH and temperature not specified in the publication

712276