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Literature summary for 2.7.4.25 extracted from

  • Bertrand, T.; Briozzo, P.; Assairi, L.; Ofiteru, A.; Bucurenci, N.; Munier-Lehmann, H.; Golinelli-Pimpaneau, B.; Barzu, O.; Gilles, A.M.
    Sugar specificity of bacterial CMP kinases as revealed by crystal structures and mutagenesis of Escherichia coli enzyme (2002), J. Mol. Biol., 315, 1099-1110.
    View publication on PubMed
Search for more literature for 2.7.4.25

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with substrates CMP, dCMP, ara-CMP and 2’,3’-dideoxy-CMP, hanging drop vapor diffusion method, using ammonium sulfate in a 50 mM Tris-HCl buffer (pH 7.4), at 20°C Escherichia coli

Protein Variants

Protein Variants Comment Organism
D185A the mutant shows severely decreased CMP and dCMP phosphoryation activity compared to the wild type enzyme Escherichia coli
R181M the mutant shows severely decreased CMP and dCMP phosphoryation activity compared to the wild type enzyme Escherichia coli
S101A the mutation reduces CMP phosphorylation only moderately, but dramatically reduces dCMP phosphorylation Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 CMP wild type enzyme, at 30°C and pH 7.4 Escherichia coli
0.08
-
 CMP mutant enzyme S101A, at 30°C and pH 7.4 Escherichia coli
0.094
-
 dCMP wild type enzyme, at 30°C and pH 7.4 Escherichia coli
0.15
-
 2',3'-dideoxy-CMP mutant enzyme D185A, at 30°C and pH 7.4 Escherichia coli
0.19
-
 CMP mutant enzyme R181M, at 30°C and pH 7.4 Escherichia coli
0.19
-
 dCMP mutant enzyme S101A, at 30°C and pH 7.4 Escherichia coli
0.24
-
 dCMP mutant enzyme D185A, at 30°C and pH 7.4 Escherichia coli
0.24
-
 dCMP mutant enzyme R181M, at 30°C and pH 7.4 Escherichia coli
0.46
-
 2',3'-dideoxy-CMP wild type enzyme, at 30°C and pH 7.4 Escherichia coli
0.47
-
 CMP mutant enzyme D185A, at 30°C and pH 7.4 Escherichia coli
0.47
-
 ara-CMP mutant enzyme S101A, at 30°C and pH 7.4 Escherichia coli
0.53
-
 ara-CMP wild type enzyme, at 30°C and pH 7.4 Escherichia coli
0.54
-
 2',3'-dideoxy-CMP mutant enzyme R181M, at 30°C and pH 7.4 Escherichia coli
0.65
-
 2',3'-dideoxy-CMP mutant enzyme S101A, at 30°C and pH 7.4 Escherichia coli
0.79
-
 ara-CMP mutant enzyme R181M, at 30°C and pH 7.4 Escherichia coli
1
-
 ara-CMP mutant enzyme D185A, at 30°C and pH 7.4 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + CMP Escherichia coli
-
 ADP + CDP
-
 ?
ATP + dCMP Escherichia coli Escherichia coli CMPK phosphorylates dCMP nearly as well as it does CMP ADP + dCDP
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli A0A140N8S7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2',3'-dideoxy-CMP 2’,3’-dideoxy-CMP is a poor substrate Escherichia coli ADP + ?
-
 ?
ATP + ara-CMP ara-CMP is a poor substrate Escherichia coli ADP + ara-CDP
-
 ?
ATP + CMP
-
 Escherichia coli ADP + CDP
-
 ?
ATP + dCMP Escherichia coli CMPK phosphorylates dCMP nearly as well as it does CMP Escherichia coli ADP + dCDP
-
 ?
ATP + UMP bacterial CMP kinases phosphorylate UMP with very low rates Escherichia coli ADP + UDP
-
 ?

Synonyms

Synonyms Comment Organism
CMP kinase
-
 Escherichia coli
CMPK
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
52
-
 the melting temperature of the wild type enzyme is at 52°C Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0083
-
 2',3'-dideoxy-CMP mutant enzyme D185A, at 30°C and pH 7.4 Escherichia coli
0.047
-
 2',3'-dideoxy-CMP wild type enzyme, at 30°C and pH 7.4 Escherichia coli
0.071
-
 dCMP mutant enzyme D185A, at 30°C and pH 7.4 Escherichia coli
0.08
-
 CMP mutant enzyme S101A, at 30°C and pH 7.4 Escherichia coli
0.085
-
 ara-CMP mutant enzyme D185A, at 30°C and pH 7.4 Escherichia coli
0.12
-
 2',3'-dideoxy-CMP mutant enzyme R181M, at 30°C and pH 7.4 Escherichia coli
0.19
-
 dCMP mutant enzyme S101A, at 30°C and pH 7.4 Escherichia coli
0.26
-
 CMP mutant enzyme D185A, at 30°C and pH 7.4 Escherichia coli
0.45
-
 dCMP mutant enzyme R181M, at 30°C and pH 7.4 Escherichia coli
0.47
-
 ara-CMP mutant enzyme S101A, at 30°C and pH 7.4 Escherichia coli
0.65
-
 2',3'-dideoxy-CMP mutant enzyme S101A, at 30°C and pH 7.4 Escherichia coli
1.36
-
 ara-CMP mutant enzyme R181M, at 30°C and pH 7.4 Escherichia coli
1.38
-
 CMP mutant enzyme R181M, at 30°C and pH 7.4 Escherichia coli
56
-
 ara-CMP wild type enzyme, at 30°C and pH 7.4 Escherichia coli
103
-
 CMP wild type enzyme, at 30°C and pH 7.4 Escherichia coli
109
-
 dCMP wild type enzyme, at 30°C and pH 7.4 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.083
-
 ara-CMP mutant enzyme D185A, at 30°C and pH 7.4 Escherichia coli K-12
0.3
-
 dCMP mutant enzyme D185A, at 30°C and pH 7.4 Escherichia coli K-12
0.54
-
 CMP mutant enzyme D185A, at 30°C and pH 7.4 Escherichia coli K-12
1.7
-
 ara-CMP mutant enzyme R181M, at 30°C and pH 7.4 Escherichia coli K-12
1.9
-
 dCMP mutant enzyme R181M, at 30°C and pH 7.4 Escherichia coli K-12
6.1
-
 dCMP mutant enzyme S101A, at 30°C and pH 7.4 Escherichia coli K-12
7.4
-
 CMP mutant enzyme R181M, at 30°C and pH 7.4 Escherichia coli K-12
7.5
-
 ara-CMP mutant enzyme S101A, at 30°C and pH 7.4 Escherichia coli K-12
105
-
 ara-CMP wild type enzyme, at 30°C and pH 7.4 Escherichia coli K-12
697
-
 CMP mutant enzyme S101A, at 30°C and pH 7.4 Escherichia coli K-12
1160
-
 dCMP wild type enzyme, at 30°C and pH 7.4 Escherichia coli K-12
2940
-
 CMP wild type enzyme, at 30°C and pH 7.4 Escherichia coli K-12