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2.7.1.71: shikimate kinase

This is an abbreviated version!
For detailed information about shikimate kinase, go to the full flat file.

Word Map on EC 2.7.1.71

Reaction

ATP
+
shikimate
=
ADP
+
3-phosphoshikimate

Synonyms

adenosine triphosphate: shikimate-3-phosphotransferase, AroK, AroL, AtSK1, AtSK2, kinase (phosphorylating), shikimate, kinase, shikimate (phosphorylating), MtSK, OsSK1, OsSK2, OsSK3, Rv2539c, shikimate kinase II, shikimate kinase-like 1, SK I, SK II, SK1, SK2, SKI, SKII, SKL1, type I shikimate kinase, aroK-encoded, type II shikimate kinase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.71 shikimate kinase

Cloned

Cloned on EC 2.7.1.71 - shikimate kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
aroK-endoded shikimate kinase, cloned and overexpressed in soluble form in Escherichia coli
-
cloning of aroK encoding shikimate kinase I. AroK protein, i.e. shikimate kinase I and AroL protein, i.e. shikimate kinase II are of comparable length and the homology between them extends the entire length of the two enzymes
-
expressed in Escherichia coli BL21 (DE3)pLysS cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BW25113 cells
-
expressed in Escherichia coli JM109 cells
expressed in Escherichia coli Rosetta (DE3) cells
expressed in Escherichia coli strain BL21(DE3)
-
expression of N-termminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3)
-
gene expression patterns of isoforms, AtSK1 and AtSK2 showing a signature of regulatory subfunctionalization
generation of knockout and antisense strain from Escherichia coli parent strain DH5alpha
-
protein is expressed in Escherichia coli as N-terminal fusion with His6 tag and a TEV-protease cleavage site
-
wild-type and mutant enzyme expressed in Escherichia coli
-