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2.7.1.71: shikimate kinase

This is an abbreviated version!
For detailed information about shikimate kinase, go to the full flat file.

Word Map on EC 2.7.1.71

Reaction

ATP
+
shikimate
=
ADP
+
3-phosphoshikimate

Synonyms

adenosine triphosphate: shikimate-3-phosphotransferase, AroK, AroL, AtSK1, AtSK2, kinase (phosphorylating), shikimate, kinase, shikimate (phosphorylating), MtSK, OsSK1, OsSK2, OsSK3, Rv2539c, shikimate kinase II, shikimate kinase-like 1, SK I, SK II, SK1, SK2, SKI, SKII, SKL1, type I shikimate kinase, aroK-encoded, type II shikimate kinase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.71 shikimate kinase

Purification

Purification on EC 2.7.1.71 - shikimate kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme aggregate contains 5 activities: EC 1.1.1.25, EC 2.7.1.71, EC 4.2.1.10, EC 4.2.3.4 and EC 2.5.1.19
-
His-Bind agarose resin column chromatography
HisTrap column chromatography, and Superdex 75 gel filtration
immobilized-nickel ion affinity chromatography and Sephacryl S-100 gel filtration
-
immobilized-nickel ion chromatography and Superdex-75 gel filtration
isoenzyme SK1 and SK2
-
Ni-NTA column chromatography
-
Ni-NTA resin column chromatography
partial
-
phenyl Sepharose column chromatography and Sephacryl S-100 gel filtration
-
recombinant N-termminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by immobilized metal affinity chromatography and dialysis
-
shikimate kinase II
-