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Literature summary for 2.7.1.71 extracted from

  • Cheng, W.C.; Chang, Y.N.; Wang, W.C.
    Structural basis for shikimate-binding specificity of Helicobacter pylori shikimate kinase (2005), J. Bacteriol., 187, 8156-8163.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Helicobacter pylori

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method using containing 0.2 M lithium sulfate, 30% (wt/vol) PEG 8000, and 0.1 M sodium acetate buffer (pH 6.5), at 20°C Helicobacter pylori
hanging-drop vapor diffusion method. 1.8 A crystal structure of shikimate kinase. The crystal structure shows a three-layer alpha/beta fold consisting of a central sheet of five parallel beta-strands flanked by seven alpha-helices. An HpSK-shikimate-PO4 complex is also determined and refined to 2.3 A, revealing induced-fit movement from an open to a closed form on substrate binding Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
shikimate in 100 mM Tris-HCl–KOH buffer, pH 7.5, 50 mM KCl, 5 mM MgCl2, 1.6 mM shikimic acid, 2.5 mM ATP, 1 mM phosphoenolpyruvate, 0.1 mM NADH, 2.5 units of pyruvate kinase/ml, and 2.7 units of lactate dehydrogenase/ml, at 25°C Helicobacter pylori
0.101
-
ATP in 100 mM Tris-HCl-KOH buffer, pH 7.5, 50 mM KCl, 5 mM MgCl2, 1.6 mM shikimic acid, 2.5 mM ATP, 1 mM phosphoenolpyruvate, 0.1 mM NADH, 2.5 units of pyruvate kinase/ml, and 2.7 units of lactate dehydrogenase/ml, at 25°C Helicobacter pylori

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
SDS-PAGE Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + shikimate Helicobacter pylori fifth step in the shikimate pathway ADP + shikimate 3-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Helicobacter pylori P56073
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized-nickel ion chromatography and Superdex-75 gel filtration Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + shikimate
-
Helicobacter pylori ADP + shikimate 3-phosphate
-
?
ATP + shikimate fifth step in the shikimate pathway Helicobacter pylori ADP + shikimate 3-phosphate
-
?

Cofactor

Cofactor Comment Organism Structure
ATP
-
Helicobacter pylori