Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Helicobacter pylori |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method using containing 0.2 M lithium sulfate, 30% (wt/vol) PEG 8000, and 0.1 M sodium acetate buffer (pH 6.5), at 20°C | Helicobacter pylori |
hanging-drop vapor diffusion method. 1.8 A crystal structure of shikimate kinase. The crystal structure shows a three-layer alpha/beta fold consisting of a central sheet of five parallel beta-strands flanked by seven alpha-helices. An HpSK-shikimate-PO4 complex is also determined and refined to 2.3 A, revealing induced-fit movement from an open to a closed form on substrate binding | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
shikimate | in 100 mM Tris-HClKOH buffer, pH 7.5, 50 mM KCl, 5 mM MgCl2, 1.6 mM shikimic acid, 2.5 mM ATP, 1 mM phosphoenolpyruvate, 0.1 mM NADH, 2.5 units of pyruvate kinase/ml, and 2.7 units of lactate dehydrogenase/ml, at 25°C | Helicobacter pylori | |
0.101 | - |
ATP | in 100 mM Tris-HCl-KOH buffer, pH 7.5, 50 mM KCl, 5 mM MgCl2, 1.6 mM shikimic acid, 2.5 mM ATP, 1 mM phosphoenolpyruvate, 0.1 mM NADH, 2.5 units of pyruvate kinase/ml, and 2.7 units of lactate dehydrogenase/ml, at 25°C | Helicobacter pylori |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Helicobacter pylori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
SDS-PAGE | Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + shikimate | Helicobacter pylori | fifth step in the shikimate pathway | ADP + shikimate 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | P56073 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized-nickel ion chromatography and Superdex-75 gel filtration | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + shikimate | - |
Helicobacter pylori | ADP + shikimate 3-phosphate | - |
? | |
ATP + shikimate | fifth step in the shikimate pathway | Helicobacter pylori | ADP + shikimate 3-phosphate | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Helicobacter pylori |