2.7.1.60: N-acylmannosamine kinase

This is an abbreviated version!
For detailed information about N-acylmannosamine kinase, go to the full flat file.

Reaction

Synonyms

acetylamidodeoxymannokinase, acetylmannosamine kinase, acylaminodeoxymannokinase, acylmannosamine kinase, acylmannosamine kinase (phosphorylating), ATP:N-acetylmannosamine 6-phosphotransferase, EC 2.7.1.59/EC 2.7.1.60, EC 5.1.3.14/EC 2.7.1.60, GNE, GNE2, GNE3, ManAc kinase, MNK, N-acetylmannosamine kinase, N-acetylmannosamine-6-phosphate 2-epimerase, N-acyl-D-mannosamine kinase, nanK, UDP-GlcNAc 2-epimerase/ManAc kinase, UDP-GlcNAc 2-epimerase/ManNAc 6-kinase, UDP-GlcNAc 2-epimerase/ManNAc kinase, UDP-GlcNAc-2 epimerase/ManNAc kinase, UDP-GlcNAc-epimerase/ManNAc kinase, UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine-kinase, UDP-N-acetylglucosamine2-epimerase/N-acetylmannosamine kinase

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.60 N-acylmannosamine kinase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
4	Activating Compound
1936	AA Sequence
3	Application
1	CAS Registry Number
26	Cloned(Commentary)
2	Cofactor
5	Crystallization (Commentary)
28	Disease/ Diagnostics
1	Expression
26	General Information
4	General Stability
24	IC50 Value
39	Inhibitors
7	kcat/KM [mM/s]
1	Ki Value [mM]
21	KM Value [mM]
21	Localization
18	Metals/Ions
23	Molecular Weight [Da]
44	Natural Substrates/ Products (Substrates)
1074	Organism
6	Pathway
17	PDB ID
4	pH Optimum
2	pH Stability
68	Protein Variants
18	Purification (Commentary)
1	Reaction
1	Reaction Type
63	Reference
74	Source Tissue
10	Specific Activity [micromol/min/mg]
6	Storage Stability
99	Substrates and Products (Substrate)
34	Subunits
92	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Stability [°C]
8	Turnover Number [1/s]

Crystallization

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Crystallization on EC 2.7.1.60 - N-acylmannosamine kinase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide Go to Crystallization (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

sitting drop vapor diffusion method, using 0.2 M lithium sulfate monohydrate, 0.1 M Tris-HCl pH 8.5, 30% (w/v) PEG 4000

Fusobacterium nucleatum subsp. nucleatum

Q8RDN7

758597

kinase domain

Homo sapiens

Q9Y223

706438

ManNAc kinase in complex with its substrate ManNAc, its product ManNAc 6-phosphate, with or without ADP, vapor diffusion at 18°C, mixing 0.002 ml of 15 mg/ml protein in 10 mM Tris-HCl, pH 8.0, 150 mM NaCl, and 2 mM ManNAc, with 0.002 ml of reservoir solution containing 0.2 M calcium acetate, 0.1 M sodiumcacodylate, pH 6.5, and 40% PEG 300, 5 days, X-ray diffraction structure determination and analysis at 1.64-2.10 A resolution. No crystals by co-crystallization of hMNK with ManNAc and ADP, ATP, AMPPCP, or AMPPNP, but ternary complexes are obtained by soaking crystals of the binary hMNK-ManNAc complex replacing stepwise their mother liquor with solutions containing 0.1 M sodium cacodylate, pH 6.5, 50% PEG 300, and 20 mM ADPor ATP or nonhydrolyzable ATP derivatives

Homo sapiens

Q9Y223

722749

ligand-free, N-acetylmannosamine-bound and substrate analog GlcNAc-bound enzyme, sitting drop vapor diffusion method, using 0.2 M sodium malonate dibasic monohydrate and 20% (w/v) PEG 3350

Staphylococcus aureus

Q2G159

759506

sitting drop vapor diffusion method, using 0.2 M ammonium chloride, 20% (w/v) polyethylene glycol 6000, 0.1 M HEPES pH 7.0

Staphylococcus aureus

-

737385