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2.7.1.60: N-acylmannosamine kinase

This is an abbreviated version!
For detailed information about N-acylmannosamine kinase, go to the full flat file.

Word Map on EC 2.7.1.60

Reaction

ATP
+
N-acyl-D-mannosamine
=
ADP
 +
N-acyl-D-mannosamine 6-phosphate

Synonyms

acetylamidodeoxymannokinase, acetylmannosamine kinase, acylaminodeoxymannokinase, acylmannosamine kinase, acylmannosamine kinase (phosphorylating), ATP:N-acetylmannosamine 6-phosphotransferase, EC 2.7.1.59/EC 2.7.1.60, EC 5.1.3.14/EC 2.7.1.60, GNE, GNE2, GNE3, ManAc kinase, MNK, N-acetylmannosamine kinase, N-acetylmannosamine-6-phosphate 2-epimerase, N-acyl-D-mannosamine kinase, nanK, UDP-GlcNAc 2-epimerase/ManAc kinase, UDP-GlcNAc 2-epimerase/ManNAc 6-kinase, UDP-GlcNAc 2-epimerase/ManNAc kinase, UDP-GlcNAc-2 epimerase/ManNAc kinase, UDP-GlcNAc-epimerase/ManNAc kinase, UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine-kinase, UDP-N-acetylglucosamine2-epimerase/N-acetylmannosamine kinase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.60 N-acylmannosamine kinase

Cloned

Cloned on EC 2.7.1.60 - N-acylmannosamine kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA cloned and sequenced, expressed in Escherichia coli HB101 or INValphaF'
cloned and functionally expressed in Escherichia coli BL21
-
cloning of isozymes hGNE1-hGNE8, DNA and amino acid sequence determination and analysis, quantitative real-time PCR expression analysis and sequence comparisons
cloning of isozymes mGNE1 and mGNE2, DNA and amino acid sequence determination and analysis, quantitative real-time PCR expression analysis and sequence comparison
expressed as His-tag fusion protein in Sf9, and Sf-900 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pS and HEK-293T cells
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
-
expressed in Escherichia coli strain DH5alpha
-
expressed in HeLa cells
-
expressed in Sf-9 cells
-
expressed in Sf9 insect cells
expressed with or without His-tag in Escherichia coli BL21 Star(DE3), Saccharomyces cerevisiae, Pichia pastoris, High five, Sf9, and Sf-900 cells, low yield in Escherichia coli and yeast, high expression of enzyme in High five, and Sf-900 cells
-
expression as His-tag fusion protein in Sf9 cells
expression in 293T cell
expression in CHO cells
-
expression of GFP-tagged enzyme in HEK-293 cells, the expression is efficiently blocked by sh70-shRNA, sh68 has no effect on GNE protein expression because its target sequence at the 3'-UTR is not present in the GFP-hGNE vector construct
-
His-tag, kinase domain expressed in Escherichia coli BL21(D3) CodonPlus-RIL
overexpression of active enzyme by using the baculovirus/Sf9 system
-
the His-tagged enzyme is expressed in Escherichia coli
-
wild type an mutated proteins expressed as Myc-tag fusion proteins in COS-7 cells
-