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2.6.1.16: glutamine-fructose-6-phosphate transaminase (isomerizing)

This is an abbreviated version!
For detailed information about glutamine-fructose-6-phosphate transaminase (isomerizing), go to the full flat file.

Word Map on EC 2.6.1.16

Reaction

L-glutamine
+
D-fructose 6-phosphate
=
L-glutamate
 +
D-glucosamine 6-phosphate

Synonyms

2-amino-2-deoxy-D-glucose-6-phosphate ketol-isomerase, BsglmS, EC 5.3.1.19, G-6-P synthase, Gfa1, Gfa1p, GFAT, GFAT1, GFAT2, GFPT1, GlcN-6-P synthase, GlmS, glmS ribozyme, glucosamine 6-phosphate synthase, glucosamine 6-phosphate synthetase, glucosamine phosphate isomerase (glutamine-forming), glucosamine synthase, glucosamine-6-P synthase, glucosamine-6-phosphate isomerase (glutamine-forming), glucosamine-6-phosphate synthase, glucosamine-6-phosphate synthetase, glucosamine-6P synthase, glucosamine:fructose-6-phosphate aminotransferase, glutamine-fructose 6-phosphate amidotransferase, glutamine-fructose 6-phosphate aminotransferase, glutamine-fructose-6-phosphate aminotransferase, glutamine-fructose-6-phosphate transaminase 1, glutamine: fructose-6-phosphate amidotransferase, glutamine: fructose-6-phosphate amidotransferase 1, glutamine: fructose-6-phosphate aminotransferase, glutamine:fructose-6-phosphate amidotransferase, glutamine:fructose-6-phosphate aminotransferase, hexosephosphate aminotransferase, isomerase, glucosamine phosphate (glutamine-forming), L-glutamine D-fructose 6-phosphate amidotransferase, L-glutamine fructose 6-phosphate transamidase, L-glutamine: D-fructose-6-phosphate amidotransferase, L-glutamine: L-fructose-6-phosphate amidotransferase, L-glutamine:D-fructose-6-phosphate amidotransferase, L-glutamine:D-fructose-6-phosphate amidotransferase (hexose isomerizing), L-glutamine:D-fructose-6-phosphate amidotransferase (hexose-isomerizing), L-glutamine:L-fructose-6-phosphate amidotransferase

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.16 glutamine-fructose-6-phosphate transaminase (isomerizing)

Specific Activity

Specific Activity on EC 2.6.1.16 - glutamine-fructose-6-phosphate transaminase (isomerizing)

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.001
-
mutant enzyme L468P
0.0047
-
-
0.021
-
glutamine 6-phosphate-synthetic activity, mutant V711F, pH 7.5, 25°C
0.05
-
glutamine 6-phosphate-synthetic activity, mutant W97G, pH 7.5, 25°C
0.095
-
amidohydrolysing activity of the mutant enzyme W97G using D-fructose 6-phosphate as substrate
0.256
-
amidohydrolysing activity of the mutant enzyme W97F using D-fructose 6-phosphate as substrate
0.39
-
wild type enzyme
0.631
-
wild type enzyme, using D-fructose 6-phosphate as a substrate
0.89
-
amidohydrolysing activity of the wild type enzyme using D-fructose 6-phosphate as substrate
0.94
-
amidohydrolysing activity of the mutant enzyme V711F using D-fructose 6-phosphate as substrate
12
-
glutamine 6-phosphate-synthetic activity, wild-type, pH 7.5, 25°C
34.8
-
-
additional information
-
development of an assay for glucosamine 6-phosphate synthase that measures the production of glucosamine 6-phosphate by either following the consumption of acetyl-CoA spectrophotometrically at 230 nm or quantifying the free thiol with 5,5'-dithio-bis(2-nitrobenzoic acid), i.e. Ellman’s reagent in a discontinuous manner. Simple assay method, which can be adapted to 96-well microtiter plate format