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0.2 - 6.5
D-fructose 6-phosphate
16
D-glucose 6-phosphate
-
pH 7.2, 37°C, isomerization catalyzed by C-terminal domain
0.67
L-gamma -glutamyl-p-nitroanilide
-
reaction mixture contains 1 mM L-gamma-glutamyl-p-nitroanilide, 1 mM EDTA, 1 mM DTT and the appropriately diluted enzyme preparation in 20 mM HEPES buffer (pH 7.5) at 25°C
0.0064 - 12.2
L-gamma-glutamyl-p-nitroanilide
additional information
additional information
-
0.2
D-fructose 6-phosphate
-
wild type enzyme
0.23
D-fructose 6-phosphate
-
calorimetric determination, pH 7.2, 37°C
0.25
D-fructose 6-phosphate
-
pH 7.5, 37°C
0.3
D-fructose 6-phosphate
-
calorimetric determination, pH 7.2, 25°C
0.36
D-fructose 6-phosphate
wild-type, 37°C
0.36
D-fructose 6-phosphate
wild type enzyme, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C
0.38
D-fructose 6-phosphate
-
pH 7.7, 37°C
0.39
D-fructose 6-phosphate
-
pH 7.0, 37°C
0.4
D-fructose 6-phosphate
-
-
0.5
D-fructose 6-phosphate
-
pH 6.3, 38°C
0.5
D-fructose 6-phosphate
synthase activity, pH 7.2, 37°C
0.52
D-fructose 6-phosphate
-
mutant S243E, 37°C, pH 7.5
0.55
D-fructose 6-phosphate
recombinant enzyme, in 10 mM KCl, 1 mg/ml bovine serum albumin, 20 mM imidazole buffer (pH 6.8), 1 mM EDTA, 1 mM dithiothreitol, and 10% (v/v) glycerol
0.67
D-fructose 6-phosphate
mutant enzyme V605L, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C
0.67
D-fructose 6-phosphate
mutant V605L, 37°C
0.75
D-fructose 6-phosphate
-
pH 7.0, 37°C
0.8
D-fructose 6-phosphate
hemisynthase activity, pH 7.2, 37°C
0.93
D-fructose 6-phosphate
mutant enzyme W74A, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C
0.93
D-fructose 6-phosphate
mutant W74A, 37°C
1.04
D-fructose 6-phosphate
-
wild-type, 37°C, pH 7.5
1.08
D-fructose 6-phosphate
-
hexose phosphate-isomerizing activity, wild-type with N-terminal His-tag, pH 7.5, 25°C
1.11
D-fructose 6-phosphate
mutant enzyme W74L, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C
1.11
D-fructose 6-phosphate
mutant W74L, 37°C
1.12
D-fructose 6-phosphate
-
mutant mutant Gfa1-His6DELTA655-660, pH 6.8, 37°C
1.15
D-fructose 6-phosphate
mutant enzyme A602L, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C
1.15
D-fructose 6-phosphate
mutant A602L, 37°C
1.2
D-fructose 6-phosphate
-
reaction mixture contains 2 mM D-fructose 6-phosphate, 0.5 mM NADP+, 1 mM EDTA, and appropriately diluted GlcN-6-P synthase preparation in 50 mM Tris/HCl buffer (pH 7.5) at 25°C
1.2
D-fructose 6-phosphate
-
wild type enzyme, in in 25 mM potassium phosphate buffer at pH 7.0 and 37°C
1.2
D-fructose 6-phosphate
-
hexose phosphate-isomerizing activity, wild-type, pH 7.5, 25°C
1.4
D-fructose 6-phosphate
-
hexose phosphate-isomerizing activity, residues 346-712, His-tagged hexose phosphate-isomerizing domain, pH 7.5, 25°C
1.41
D-fructose 6-phosphate
-
glutamine 6-phosphate-synthetic activity, wild-type, pH 7.5, 25°C
1.45
D-fructose 6-phosphate
mutant enzyme W74F, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C
1.45
D-fructose 6-phosphate
mutant W74F, 37°C
1.56
D-fructose 6-phosphate
-
wild-type, pH 6.8, 37°C
1.9
D-fructose 6-phosphate
-
mutant mutant Gfa1-K568H/S569H, pH 6.8, 37°C
2
D-fructose 6-phosphate
-
pH 7.5, 37°C
2
D-fructose 6-phosphate
-
pH 7.0, 37°C
2.6
D-fructose 6-phosphate
-
mutant Gfa1-His6DELTA343-348, pH 6.8, 37°C
2.8
D-fructose 6-phosphate
pH 7.0, 37°C
3.8
D-fructose 6-phosphate
-
pH 6.5, 30°C
3.8
D-fructose 6-phosphate
-
pH 7.2, 37°C, isomerization catalyzed by C-terminal domain
6.5
D-fructose 6-phosphate
-
hexose phosphate-isomerizing activity, wild-type with C-terminal His-tag, pH 7.5, 25°C
0.0064
L-gamma-glutamyl-p-nitroanilide
mutant W74F, 37°C
0.05
L-gamma-glutamyl-p-nitroanilide
wild-type, 37°C
0.1
L-gamma-glutamyl-p-nitroanilide
mutant W74L, 37°C
0.2
L-gamma-glutamyl-p-nitroanilide
mutant W74A, 37°C
0.29
L-gamma-glutamyl-p-nitroanilide
mutant V605L, 37°C
0.31
L-gamma-glutamyl-p-nitroanilide
-
residues 1-345, His-tagged glutamine amide-hydrolysing domain, pH 7.5, 25°C
0.36
L-gamma-glutamyl-p-nitroanilide
mutant A602L, 37°C
0.64
L-gamma-glutamyl-p-nitroanilide
-
wild-type with C-terminal His-tag, pH 7.5, 25°C
0.67
L-gamma-glutamyl-p-nitroanilide
-
wild-type, pH 7.5, 25°C
1.8
L-gamma-glutamyl-p-nitroanilide
-
both wild-type and mutant S243E, 37°C, pH 7.5
12.2
L-gamma-glutamyl-p-nitroanilide
-
wild-type with N-terminal His-tag, pH 7.5, 25°C
0.2
L-glutamate
-
calorimetric determination, pH 7.2, 25°C
0.34
L-glutamate
-
reaction mixture contains 10 mM L-glutamate, 1 mM EDTA, 1 mM DTT and an appropriately diluted enzyme preparation in 20 mM HEPES buffer (pH 7.5) at 25°C
1.33
L-glutamate
-
mutant Gfa1-K568H/S569H, pH 6.8, 37°C
1.36
L-glutamate
-
mutant Gfa1-His6DELTA343-348, pH 6.8, 37°C
1.41
L-glutamate
-
wild-type, pH 6.8, 37°C
2.36
L-glutamate
-
mutant Gfa1-His6DELTA655-660, pH 6.8, 37°C
0.04
L-glutamine
mutant enzyme W74L, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C
0.04
L-glutamine
mutant W74L, 37°C
0.048
L-glutamine
mutant enzyme W74F, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C
0.048
L-glutamine
mutant W74F, 37°C
0.072
L-glutamine
mutant enzyme V605L, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C
0.072
L-glutamine
mutant V605L, 37°C
0.1
L-glutamine
mutant enzyme A602L, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C
0.1
L-glutamine
mutant A602L, 37°C
0.11
L-glutamine
synthase activity, pH 7.2, 37°C
0.14
L-glutamine
hemisynthase activity, pH 7.2, 37°C
0.17
L-glutamine
-
wild type enzyme
0.2
L-glutamine
-
pH 7.5, 37°C
0.2
L-glutamine
-
pH 7.4, 37°C
0.225
L-glutamine
-
pH 7.5, 37°C
0.27
L-glutamine
wild-type, 37°C
0.27
L-glutamine
wild type enzyme, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C
0.3
L-glutamine
-
pH 7.5, 37°C
0.3
L-glutamine
-
pH 6.3, 38°C
0.34
L-glutamine
-
glutamine 6-phosphate-synthetic activity, wild-type, pH 7.5, 25°C
0.38
L-glutamine
mutant enzyme W74A, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C
0.38
L-glutamine
mutant W74A, 37°C
0.4
L-glutamine
-
pH 7.5, 37°C
0.5
L-glutamine
-
pH 6.5, 30°C
0.6
L-glutamine
-
pH 7.5, 37°C
0.65
L-glutamine
-
pH 7.0, 37°C
0.74
L-glutamine
-
pH 7.0, 37°C
0.75
L-glutamine
recombinant enzyme, in 10 mM KCl, 1 mg/ml bovine serum albumin, 20 mM imidazole buffer (pH 6.8), 1 mM EDTA, 1 mM dithiothreitol, and 10% (v/v) glycerol
0.85
L-glutamine
-
pH 7.5, 37°C
1.6
L-glutamine
-
pH 7.7, 37°C
5.1
L-glutamine
pH 7.0, 55°C
additional information
additional information
thermodynamics
-
additional information
additional information
binding energy calculations of wild-type and mutant enzymes
-
additional information
additional information
-
binding energy calculations of wild-type and mutant enzymes
-