Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.6.1.16: glutamine-fructose-6-phosphate transaminase (isomerizing)

This is an abbreviated version!
For detailed information about glutamine-fructose-6-phosphate transaminase (isomerizing), go to the full flat file.

Word Map on EC 2.6.1.16

Reaction

L-glutamine
+
D-fructose 6-phosphate
=
L-glutamate
 +
D-glucosamine 6-phosphate

Synonyms

2-amino-2-deoxy-D-glucose-6-phosphate ketol-isomerase, BsglmS, EC 5.3.1.19, G-6-P synthase, Gfa1, Gfa1p, GFAT, GFAT1, GFAT2, GFPT1, GlcN-6-P synthase, GlmS, glmS ribozyme, glucosamine 6-phosphate synthase, glucosamine 6-phosphate synthetase, glucosamine phosphate isomerase (glutamine-forming), glucosamine synthase, glucosamine-6-P synthase, glucosamine-6-phosphate isomerase (glutamine-forming), glucosamine-6-phosphate synthase, glucosamine-6-phosphate synthetase, glucosamine-6P synthase, glucosamine:fructose-6-phosphate aminotransferase, glutamine-fructose 6-phosphate amidotransferase, glutamine-fructose 6-phosphate aminotransferase, glutamine-fructose-6-phosphate aminotransferase, glutamine-fructose-6-phosphate transaminase 1, glutamine: fructose-6-phosphate amidotransferase, glutamine: fructose-6-phosphate amidotransferase 1, glutamine: fructose-6-phosphate aminotransferase, glutamine:fructose-6-phosphate amidotransferase, glutamine:fructose-6-phosphate aminotransferase, hexosephosphate aminotransferase, isomerase, glucosamine phosphate (glutamine-forming), L-glutamine D-fructose 6-phosphate amidotransferase, L-glutamine fructose 6-phosphate transamidase, L-glutamine: D-fructose-6-phosphate amidotransferase, L-glutamine: L-fructose-6-phosphate amidotransferase, L-glutamine:D-fructose-6-phosphate amidotransferase, L-glutamine:D-fructose-6-phosphate amidotransferase (hexose isomerizing), L-glutamine:D-fructose-6-phosphate amidotransferase (hexose-isomerizing), L-glutamine:L-fructose-6-phosphate amidotransferase

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.16 glutamine-fructose-6-phosphate transaminase (isomerizing)

Storage Stability

Storage Stability on EC 2.6.1.16 - glutamine-fructose-6-phosphate transaminase (isomerizing)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18°C, little loss of activity after 1 week for lyophilized material after calcium phosphate gel treatment
-
-20°C, 0.6 M sucrose, 60% loss of activity after 45 days
-
-20°C, potassium phosphate buffer, pH 7.0, containing 1 mM EDTA, 1 mM DTT, 50% glycerol and 10 mM fructose 6-phosphate, fully stable
-25°C, 1 month, 15% activity loss, ammonium sulfate precipitated enzyme
-
-80°C, 3 months
-
-80°C, 50 mM Tris-HCl, pH 7.8, 200 mM NaCl, 1 mM fructose 6-phosphate, 2 mM tris(2-carboxyethyl)phosphine hydrochloride, 200 mM imidazole, 10% glycerol, stable for up to 12 months
-
0-2°C, potassium phosphate buffer, pH 7.0, 0.06 M L-glutamine, 0.01 M EDTA, stable for up to 3 or 4 weeks
-
4°C or -15°C, 8 days, 50% activity loss
-
4°C or 15°C, 50% loss of activity after 8 days
-
4°C, 25 mM potassium phosphate buffer, pH 7.5, 1 mM EGTA, 2 mM dithiothreitol, 500 mM sucrose, stable for up to 2 or 3 weeks
-
4°C, 50 mM MOPS (pH 6.5), 1 mM dithiothreitol, 1 mM EDTA, and 10 mM KCl, overnight storage, recombinant enzyme activity is low and no longer evident with preparations losing 60% of their activity
4°C, potassium phosphate buffer, pH 7.0, containing 1 mM EDTA, 1 mM DTT, 50% glycerol and 10 mM fructose 6-phosphate, complete loss of activity within 24 h
5°C, potassium phosphate buffer, pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, 0.5 mM L-glutamine, 0.05 mM D-glucose 6-phosphate, 600 mM sucrose, stable for few weeks
-
in liquid N2, stable for up to 2 weeks
-