2.5.1.20: rubber cis-polyprenylcistransferase

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For detailed information about rubber cis-polyprenylcistransferase, go to the full flat file.

Reaction

Synonyms

allyltransferase, rubber, chain-elongating enzyme cis-prenyltransferase, cis prenyl transferase, cis-1,4-polyprenylcistransferase, cis-prenyl transferase, cis-prenyltransferase, cisprenyltransferase, CPT, CPT3, CPT6, HRT1, HRT2, isopentenyl pyrophosphate cis-1,4-polyisoprenyl transferase, laticifer-specific rubber-forming enzyme, More, RT-ase complex, rTase, rubber allyltransferase, rubber polymerase, rubber prenyltransferase, rubber transferase, rubber transferase 1, rubber transferase 2, rubber transferase complex, RubCPT1, TkCPT1, TkCPT2, TkCPT3, TkCPTL1

ECTree

     2 Transferases

         2.5 Transferring alkyl or aryl groups, other than methyl groups

             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

                2.5.1.20 rubber cis-polyprenylcistransferase

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Results	in table
19	Activating Compound
12	AA Sequence
3	Application
1	CAS Registry Number
6	Cloned(Commentary)
2	Cofactor
9	Expression
18	General Information
26	Inhibitors
8	Ki Value [mM]
18	KM Value [mM]
25	Localization
45	Metals/Ions
10	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
67	Organism
1	Oxidation Stability
2	Pathway
1	pH Optimum
1	pH Stability
2	Protein Variants
10	Purification (Commentary)
1	Reaction
1	Reaction Type
62	Reference
45	Source Tissue
4	Specific Activity [micromol/min/mg]
1	Storage Stability
77	Substrates and Products (Substrate)
7	Subunits
71	Synonyms
1	Systematic Name
14	Temperature Optimum [°C]
3	Temperature Stability [°C]

Specific Activity

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Specific Activity on EC 2.5.1.20 - rubber cis-polyprenylcistransferase

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0.0000026

Taraxacum kok-saghyz

-

2-methyl-1,3-butadiene incorporation assay with rubber particles, 100 mM Tris-HCl, pH 7.5, 30°C, 2 h

707862

0.000003

Taraxacum kok-saghyz

-

2-methyl-1,3-butadiene incorporation assay with rubber particles, 100 mM Tris-HCl, pH 7.5, 30°C, 2 h

707862

0.000695

Parthenium argentatum

-

partially purified enzyme

638730

additional information

Hevea brasiliensis

-

2.3 microM isopentenyl diphosphate incorporation/g dry rubber/4 h for small rubber particles, 0.7 microM isopentenyl diphosphate incorporation/g dry rubber/4 h for purified and washed small rubber particles, almost no isopentenyl diphosphate incorporation/g dry rubber/4 h for washed and highly purified small rubber particles, in vitro, 50 mM phosphate buffer, pH 7.5, 40 mM 2-mercaptoethanol, 40 mM KF, 8 mM MgCl2 + substrates, 30°C, small rubber particle activity decreases with increasing dimethylallyl diphosphate concentration, dimethylallyl diphosphate slightly increases activity in washed small particles not in a dose-dependent manner

709187