2.5.1.20: rubber cis-polyprenylcistransferase
This is an abbreviated version!
For detailed information about rubber cis-polyprenylcistransferase, go to the full flat file.
Word Map on EC 2.5.1.20
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2.5.1.20
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dolichols
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brasiliensis
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hevea
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allylic
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undecaprenyl
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parthenium
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argentatum
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polyprenols
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isoprene
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prenyltransferases
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guayule
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polyisoprenoids
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cis-1,4-polyisoprene
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trans-prenyltransferases
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ficus
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dimethylallyl
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rubber-producing
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nogo-b
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elastica
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neryl
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dehydrodolichyl
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dhdds
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cis-polyisoprene
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molecular biology
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synthesis
- 2.5.1.20
- dolichols
- brasiliensis
- hevea
-
allylic
- undecaprenyl
- parthenium
- argentatum
- polyprenols
- isoprene
- prenyltransferases
-
guayule
-
polyisoprenoids
- cis-1,4-polyisoprene
- trans-prenyltransferases
- ficus
-
dimethylallyl
-
rubber-producing
- nogo-b
- elastica
-
neryl
-
dehydrodolichyl
- dhdds
-
cis-polyisoprene
- molecular biology
- synthesis
Reaction
Synonyms
allyltransferase, rubber, chain-elongating enzyme cis-prenyltransferase, cis prenyl transferase, cis-1,4-polyprenylcistransferase, cis-prenyl transferase, cis-prenyltransferase, cisprenyltransferase, CPT, CPT3, CPT6, HRT1, HRT2, isopentenyl pyrophosphate cis-1,4-polyisoprenyl transferase, laticifer-specific rubber-forming enzyme, More, RT-ase complex, rTase, rubber allyltransferase, rubber polymerase, rubber prenyltransferase, rubber transferase, rubber transferase 1, rubber transferase 2, rubber transferase complex, RubCPT1, TkCPT1, TkCPT2, TkCPT3, TkCPTL1
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Metals Ions
Metals Ions on EC 2.5.1.20 - rubber cis-polyprenylcistransferase
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Mg2+
Mn2+
Mg2+
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magnesium compounds essential for full activity, cannot be replaced by manganese, cobalt or iron
Mg2+
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activating above and below 8 mM, metal ion concentration regulates rubber initiation rate, biosythetic rate and molecular weight of product
Mg2+
recombinant HRT2 (and not HRT1) can synthesize natural rubber in the presence of washed latex rubber particles from Hevea, isopentenyl diphosphate, and farnesyl pyrophosphate, and divalent cations, such as Mg2+ and Mn2+
Mg2+
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highest rate of rubber biosynthesis at 40 mM, the affinity of the rubber transferase for isopentenyl diphosphate is highly dependent on Mg2+
Mg2+
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2 to 5 mM MgCl2 increase 2-methyl-1,3-butadiene incorporation, optimum 4-5 mM, with 7.5 mM decline below the 2 mM value, almost loss of activity with 10 mM MgCl2
Mg2+
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increase in in 2-methyl-1,3-butadiene incorporation from 2 up to 5 mM MgCl2, optimum 4-5 mM, with 7.5 mM decline below the 2 mM value, almost loss of activity with 10 mM MgCl2
Mn2+
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metal ion concentration regulates rubber initiation rate, biosythetic rate and molecular weight of product
Mn2+
recombinant HRT2 (and not HRT1) can synthesize natural rubber in the presence of washed latex rubber particles from Hevea, isopentenyl diphosphate, and farnesyl pyrophosphate, and divalent cations, such as Mg2+ and Mn2+