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2.5.1.20: rubber cis-polyprenylcistransferase

This is an abbreviated version!
For detailed information about rubber cis-polyprenylcistransferase, go to the full flat file.

Word Map on EC 2.5.1.20

Reaction

poly-cis-polyprenyl diphosphate
+
isopentenyl diphosphate
=
diphosphate
+
a poly-cis-polyprenyl diphosphate longer by one C5 unit

Synonyms

allyltransferase, rubber, chain-elongating enzyme cis-prenyltransferase, cis prenyl transferase, cis-1,4-polyprenylcistransferase, cis-prenyl transferase, cis-prenyltransferase, cisprenyltransferase, CPT, CPT3, CPT6, HRT1, HRT2, isopentenyl pyrophosphate cis-1,4-polyisoprenyl transferase, laticifer-specific rubber-forming enzyme, More, RT-ase complex, rTase, rubber allyltransferase, rubber polymerase, rubber prenyltransferase, rubber transferase, rubber transferase 1, rubber transferase 2, rubber transferase complex, RubCPT1, TkCPT1, TkCPT2, TkCPT3, TkCPTL1

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.20 rubber cis-polyprenylcistransferase

Cloned

Cloned on EC 2.5.1.20 - rubber cis-polyprenylcistransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21AI cells and in Nicotiana tabacum leaves
expression in Saccharomyces cerevisiae SNH23-D7 (temperature sensitive mutant strain for dehydrodoichyl diphosphate synthase) or Nicotiana tabacum protoplasts, enzyme remains fully functional, and expression in Escherichai coli BL21 for sequencing and Western blot analysis with antibodies
-
expression of isozymes TbCPT1, TbCPT2, and TbCPT3 associated with rubber particles in transgenic Taraxacum brevicorniculatum plants
-
recombinant expression of C-terminally His6-tagged HRT2 in Escherichia coli, expression of C-terminally His6-tagged HRT1 amd HRT2 in Saccharomyces cerevisiae and Arabidopsis thaliana T87 cultured cells, predominantly in the membrane fraction in yeast cells
-
transient expression in Nicotiana benthamiana leaves