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2.5.1.20: rubber cis-polyprenylcistransferase

This is an abbreviated version!
For detailed information about rubber cis-polyprenylcistransferase, go to the full flat file.

Word Map on EC 2.5.1.20

Reaction

poly-cis-polyprenyl diphosphate
+
isopentenyl diphosphate
=
diphosphate
+
a poly-cis-polyprenyl diphosphate longer by one C5 unit

Synonyms

allyltransferase, rubber, chain-elongating enzyme cis-prenyltransferase, cis prenyl transferase, cis-1,4-polyprenylcistransferase, cis-prenyl transferase, cis-prenyltransferase, cisprenyltransferase, CPT, CPT3, CPT6, HRT1, HRT2, isopentenyl pyrophosphate cis-1,4-polyisoprenyl transferase, laticifer-specific rubber-forming enzyme, More, RT-ase complex, rTase, rubber allyltransferase, rubber polymerase, rubber prenyltransferase, rubber transferase, rubber transferase 1, rubber transferase 2, rubber transferase complex, RubCPT1, TkCPT1, TkCPT2, TkCPT3, TkCPTL1

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.20 rubber cis-polyprenylcistransferase

Expression

Expression on EC 2.5.1.20 - rubber cis-polyprenylcistransferase

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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
CPT expression is reduced by laticifer-specific RNA interference, CPT-RNAi
-
expression of rubber transferase is up-regulated by methyl jasmonate treatment
-
HbRZFP1 expression leads to suppression of expression of rubber transferase gene HRT2. HbRZFP1 may be a negative transcription regulator of HRT2 that is involved in natural rubber biosynthesis in Hevea brasiliensis
induced by cold-stress
recombinant expression of HRT2 in Escherichia coli is significantly activated in the presence of a centrifuged latex fraction, resulting in the formation of polyisoprenes corresponding to natural rubber
-
rubber yields and/or yield characteristics are significantly positively correlated with enzyme expression levels. The up-regulation of the gene might be related to the latex production of rubber trees under the stress of bark tapping and latex metabolism. Both bark tapping and ethephon and methyl jasmonate stimulation could significantly increase the transcript levels, which shows the greatest expression levels during the last stage of incipient tapping after tree opening
the 5'UTR intron of the promoter of the rubber transferase gene (HRT2) suppresses the expression of HRT2
the enzyme shows the highest level of expression in the latex of the flowering stage
the presence of the 14-3-3 protein HbGF14a, may be sufficient to activate natural rubber biosynthesis