2.4.2.38: glycoprotein 2-beta-D-xylosyltransferase
This is an abbreviated version!
For detailed information about glycoprotein 2-beta-D-xylosyltransferase, go to the full flat file.
Word Map on EC 2.4.2.38
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2.4.2.38
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proteoglycans
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glycosaminoglycans
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desbuquois
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alpha1,3-fucosyltransferase
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laxity
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2.4.2.26
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udp-xylose
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chain-initiating
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xylosylated
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medicine
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alpha1,3-fucose
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biotechnology
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beta1,2-xylose
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o-xylosyltransferase
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synthesis
- 2.4.2.38
- proteoglycans
- glycosaminoglycans
-
desbuquois
-
alpha1,3-fucosyltransferase
-
laxity
-
2.4.2.26
- udp-xylose
-
chain-initiating
-
xylosylated
- medicine
-
alpha1,3-fucose
- biotechnology
-
beta1,2-xylose
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o-xylosyltransferase
- synthesis
Reaction
Synonyms
beta 1,2-xylosyltransferase, beta-1,2-xylosyltransferase, beta-1,2-XylT, beta-1,4-mannosylglycoprotein beta-1,2-xylosyltransferase, beta1,2-xylosyltransferase, CAP3, Cryptococcal xylosyltransferase, cryptococcus xylosyltransferase 1, CXT1, Cxt1p, CXT1p homolog, RCN11, XT-I, XT1, Xt1p, Xyl-beta-1,2-transferase, xylan xylosyltransferase1, xylosyltransferase I, xylosyltransferase-I, XylT, XYLT1, XYXT1
ECTree
2.4.2.38 glycoprotein 2-beta-D-xylosyltransferaseAdvanced search results
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results (Cloned
Cloned on EC 2.4.2.38 - glycoprotein 2-beta-D-xylosyltransferase
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CLONED (Commentary) 
ORGANISM
UNIPROT
LITERATURE
5-kb PCR product cloned into the TOPO pCR2.1 vector and maintained in Escherichia coli DH5alpha
amplification of the gene with flanking regions (1.3 kb upstream of the start codon, 1 kb downstream of the gene) from serotype D (strain JEC21) genomic DNA for cloning in pCR2.1TOPO, generation of replacement cassettes for replacing endogenous CXT1p: (i) nourseothricin acetyltransferase resistance gene flanked by the CXT1p flanking regions, transformed as linear DNA in CAP67 strain, (ii) cxt1 gene lacking 3 two-thirds of the encoding sequence flanked by its flanking regions, transformed as linear DNA in JEC21 strain
DNA and amino acid sequence determination and analysis, expression of full length wild-type enzyme and diverse mutants in Spodoptera frugiperda Sf9 insect cells via baculovirus infection system as soluble proteins lacking the transmembrane domain, expression of the full length enzyme and enzyme fragments as GFP-fusion proteins in tobacco cells Golgi medial cisternae via Agrobacterium tumefaciens infection system for study of enzyme subcellular localization
DNA and amino acid sequence determination and analysis, gene is located on chromosome 8, functional expression of the enzyme in Pichia pastoris as soluble protein, secretion to the cell culture medium
DNA and amino acid sequence determination and analysis, genetic organization and structure, functional expression in Spodoptera frugiperda Sf21 cells using the baculovirus transfection system
expressed in Arabidopsis thaliana gux1/2/3 triple mutant and HEK-293F cells
expression of N-terminally His5-tagged enzyme in Pichia pastoris, recombinant enzyme is secreted to the medium
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expression of the enzyme's isolated CTS region, N-terminal 90 amino acids, and of the T and C region separately, as fusion protein with GFP in Nicotiana benthamiana leaf epidermal cells, the CT region, but not only the T or C regions, is sufficient to sustain Golgi retention without the involvement of any luminal sequences
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expression of wild-type and mutant enzymes in Spodoptera frugiperda SF21 cells using the baculovirus infection system
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from JEC21 cDNA by PCR with primers introducing C-terminal hexa-His tag, in pCR2.1 TOPO for sequencing, in pPGK (cloned between promoter and terminator of phosphoglycerate kinase) for expression in Saccharomyces cerevisiae that lacks endogenous xylosyltransferase activity
gene xylt, transfection of chimeric constructs via Agrobacterium tumefaciens strain C58Z707, overview
