2.4.2.38: glycoprotein 2-beta-D-xylosyltransferase

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For detailed information about glycoprotein 2-beta-D-xylosyltransferase, go to the full flat file.

Word Map on EC 2.4.2.38

2.4.2.38

proteoglycans

glycosaminoglycans

desbuquois

alpha1,3-fucosyltransferase

laxity

2.4.2.26

udp-xylose

chain-initiating

xylosylated

medicine

alpha1,3-fucose

biotechnology

beta1,2-xylose

o-xylosyltransferase

synthesis

Reaction

N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein]

N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-[beta-D-Xyl-(1->2)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein]

Synonyms

beta 1,2-xylosyltransferase, beta-1,2-xylosyltransferase, beta-1,2-XylT, beta-1,4-mannosylglycoprotein beta-1,2-xylosyltransferase, beta1,2-xylosyltransferase, CAP3, Cryptococcal xylosyltransferase, cryptococcus xylosyltransferase 1, CXT1, Cxt1p, CXT1p homolog, RCN11, XT-I, XT1, Xt1p, Xyl-beta-1,2-transferase, xylan xylosyltransferase1, xylosyltransferase I, xylosyltransferase-I, XylT, XYLT1, XYXT1

ECTree

2 Transferases

2.4 Glycosyltransferases

2.4.2 Pentosyltransferases

2.4.2.38 glycoprotein 2-beta-D-xylosyltransferase

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Results	in table
46	AA Sequence
8	Application
1	CAS Registry Number
15	Cloned(Commentary)
44	Disease/ Diagnostics
4	Expression
4	General Information
1	General Stability
14	Inhibitors
2	Ki Value [mM]
2	KM Value [mM]
13	Localization
11	Metals/Ions
8	Molecular Weight [Da]
23	Natural Substrates/ Products (Substrates)
40	Organism
3	Pathway
8	pH Optimum
1	pH Range
2	pH Stability
4	Posttranslational Modification
12	Protein Variants
5	Purification (Commentary)
4	Reaction
19	Reaction Type
37	Reference
14	Source Tissue
5	Specific Activity [micromol/min/mg]
3	Storage Stability
53	Substrates and Products (Substrate)
4	Subunits
68	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]

Purification

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Purification on EC 2.4.2.38 - glycoprotein 2-beta-D-xylosyltransferase

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2 entries

endogenous activity (2967-fold purification) from serotype D ags1delta strain: solubilisation from crude membranes with 1% Triton X-100 (on ice, 30 min), HiPrep 16/10 Q-Sepharose chromatography with NaCl gradient elution (pH 8), activity peaks were pooled and concentrated (spin, 10000 Da cut-off), HiPrep 26/60 Sephacryl S-300 gel filtration chromatography with isocratic gradient elution (pH 8), concanavalin A-Sepharose 4B chromatography with alpha-methyl-mannoside elution (incubation over night, 4°C), HiTrap DEAE chromatorgraphy with NaCl gradient elution (pH 8), alpha-(1,3)-mannobiose agarose resin affinity chromatography with NaCl gradient (pH 6.5), activity peaks were combined and concentrated, overexpressed hexa-His tagged enzyme: solubilisation, Talon Superflow cobalt metal affinity chromatography, elution with 250 mM imidazole, pH 7.5

Cryptococcus neoformans

Q5K8R6

692993

recombinant N-terminally His5-tagged enzyme from Pichia pastoris cell medium by nickel chelating affinity chromatography and ultrafiltration to homogeneity

Arabidopsis thaliana

658226

subcellular fractionation

Arabidopsis thaliana

660192