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Information on EC 2.4.2.38 - glycoprotein 2-beta-D-xylosyltransferase
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EC Tree
2.4.2.38 glycoprotein 2-beta-D-xylosyltransferaseIUBMB Comments
Specific for N-linked oligosaccharides (N-glycans).
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Word Map
- 2.4.2.38
- proteoglycans
- glycosaminoglycans
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desbuquois
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alpha1,3-fucosyltransferase
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laxity
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2.4.2.26
- udp-xylose
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chain-initiating
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xylosylated
- medicine
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alpha1,3-fucose
- biotechnology
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beta1,2-xylose
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o-xylosyltransferase
- synthesis
The enzyme appears in viruses and cellular organisms
Reaction Schemes
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Synonyms
xylt1, xylosyltransferase i, rcn11, xylosyltransferase-i, beta1,2-xylosyltransferase, cxt1p, xyxt1, beta-1,2-xylosyltransferase, cryptococcal xylosyltransferase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
beta 1,2-xylosyltransferase
beta-1,2-xylosyltransferase
beta-1,4-mannosylglycoprotein beta-1,2-xylosyltransferase
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beta1,2-xylosyltransferase
Cxt1p
CXT1p homolog
XylT
beta-1,2-xylosyltransferase
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CXT1p homolog
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12 to 23% identity to CTX1p from Cryptococcus neoformans serotype D
CXT1p homolog
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12 to 23% identity to CTX1p from Cryptococcus neoformans serotype D
CXT1p homolog
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12 to 23% identity to CTX1p from Cryptococcus neoformans serotype D
CXT1p homolog
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12 to 23% identity to CTX1p from Cryptococcus neoformans serotype D
CXT1p homolog
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12 to 23% identity to CTX1p from Cryptococcus neoformans serotype D
CXT1p homolog
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12 to 23% identity to CTX1p from Cryptococcus neoformans serotype D
CXT1p homolog
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12 to 23% identity to CTX1p from Cryptococcus neoformans serotype D
CXT1p homolog
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group of 5 genes (CAP1, CAP5, CAP2, CAP4, CAP10) with 20 to 30% identity to CTX1p
CXT1p homolog
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12 to 23% identity to CTX1p from Cryptococcus neoformans serotype D
CXT1p homolog
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12 to 23% identity to CTX1p from Cryptococcus neoformans serotype D
CXT1p homolog
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12 to 23% identity to CTX1p from Cryptococcus neoformans serotype D
CXT1p homolog
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12 to 23% identity to CTX1p from Cryptococcus neoformans serotype D
XylT
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-xylose + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein] = UDP + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-[beta-D-Xyl-(1->2)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein]
UDP-D-xylose + N4-{N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl}asparagine = UDP + N4-{N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-[beta-D-xylosyl-(1,2)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl}asparagine
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UDP-D-xylose + N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl}asparagine = UDP + N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-[beta-D-xylosyl-(1->2)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl}asparagine
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UDP-alpha-D-xylose + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein] = UDP + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-[beta-D-Xyl-(1->2)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein]
the catalytic domain of XylT encompasses residues Lys57-Cys534
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UDP-alpha-D-xylose + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein] = UDP + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-[beta-D-Xyl-(1->2)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein]
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
transfer of glycosyl group
transfer of glycosyl group
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transfer of glycosyl group
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N-glycan maturation, transfer of beta (1,2)-xylose
transfer of glycosyl group
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transfer of beta (1,2)-xylose, capsule synthesis
transfer of glycosyl group
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transfer of beta (1,2)-xylose, capsule synthesis
transfer of glycosyl group
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transfer of beta (1,2)-xylose, capsule synthesis
transfer of glycosyl group
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transfer of beta (1,2)-xylose, capsule synthesis
transfer of glycosyl group
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transfer of beta (1,2)-xylose, capsule synthesis
transfer of glycosyl group
transfer of beta (1,2)-xylose, capsule synthesis
transfer of glycosyl group
transfer of beta (1,2)-xylose, capsule synthesis
transfer of glycosyl group
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transfer of beta (1,2)-xylose, capsule synthesis
transfer of glycosyl group
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transfer of beta (1,2)-xylose, capsule synthesis
transfer of glycosyl group
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transfer of beta (1,2)-xylose, capsule synthesis
transfer of glycosyl group
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transfer of beta (1,2)-xylose, capsule synthesis
transfer of glycosyl group
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transfer of beta (1,2)-xylose, capsule synthesis
transfer of glycosyl group
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transfer of beta (1,2)-xylose, capsule synthesis
transfer of glycosyl group
transfer of beta (1,2)-xylose, capsule synthesis, yeast specific
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SYSTEMATIC NAME
IUBMB Comments
UDP-D-xylose:glycoprotein (D-xylose to the 3,6-disubstituted mannose of N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl}asparagine) 2-beta-D-xylosyltransferase
Specific for N-linked oligosaccharides (N-glycans).
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CAS REGISTRY NUMBER
COMMENTARY
141256-56-6
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-O-octyl + UDP-xylose
?
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i.e. GnGn-octyl
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?
UDP-alpha-D-xylose + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein]
UDP + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-[beta-D-Xyl-(1->2)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein]
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?
UDP-alpha-D-xylose + xylan
?
the enzyme adds side chain xylosyl residues directly to the xylan backbone at 2-O
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?
UDP-D-xylose + alpha-D-mannosyl-(1,6)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)]-beta-D-mannosyl-O-(CH2)7-CH3
UDP + ?
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?
UDP-D-xylose + D-mannosyl-alpha-(1,3)-D-mannose
UDP + D-xylosyl-beta-(1,2)-D-mannosyl-alpha-(1,3)-D-mannose
UDP-D-xylose + galactoxylomannan
UDP + [beta-D-xylose-(1,2)]-galactoxylomannan
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JEC21/cxt1delta and CAP67/cxt1delta lacking CXT1p: almost complete loss of beta-xylose residues (1,2)-linked to mannose, unchanged amounts of beta-xylose (1,3)-linked to galactose
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?
UDP-D-xylose + galactoxylomannan
UDP + [beta-D-xylosyl-(1,2)]-galactoxylomannan
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?
UDP-D-xylose + GlcNAcbeta1-2Manalpha1-3Manbeta1-O-(CH2)7-CH3
UDP + GlcNAcbeta1-2Manalpha1-3(Xylbeta1-2)Manbeta1-O-(CH2)7-CH3
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-
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?
UDP-D-xylose + GlcNAcbeta1-2Manalpha1-6(Galbeta1-4GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAc
UDP + GlcNAcbeta1-2Manalpha1-6(Galbeta1-4GlcNAcbeta1-2Manalpha1-3)(Xylbeta1-2)Manbeta1-4GlcNAc
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?
UDP-D-xylose + GlcNAcbeta1-2Manalpha1-6(Galbeta1-4GlcNAcbeta1-2Manalpha1-3)Manbeta1-O-(CH2)7-CH3
UDP + GlcNAcbeta1-2Manalpha1-6(Galbeta1-4GlcNAcbeta1-2Manalpha1-3)(Xylbeta1-2)Manbeta1-O-(CH2)7-CH3
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?
UDP-D-xylose + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)GlcNAc1-O-(N-benzyloxycarbonyl)-Tyr
UDP + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)(Xylbeta1-2)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)GlcNAc1-O-(N-benzyloxycarbonyl)-Tyr
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?
UDP-D-xylose + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc
UDP + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)(Xylbeta1-2)Manbeta1-4GlcNAcbeta1-4GlcNAc
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?
UDP-D-xylose + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-Asn
UDP + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)(Xylbeta1-2)Manbeta1-4GlcNAcbeta1-4GlcNAc-Asn
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?
UDP-D-xylose + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc1-O-(N-benzyloxycarbonyl)-Tyr
UDP + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)(Xylbeta1-2)Manbeta1-4GlcNAcbeta1-4GlcNAc1-O-(N-benzyloxycarbonyl)-Tyr
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?
UDP-D-xylose + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc1-O-(pyrid-2-yl)amine
UDP + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)(Xylbeta1-2)Manbeta1-4GlcNAcbeta1-4GlcNAc1-O-(pyrid-2-yl)amine
UDP-D-xylose + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-O-(CH2)7-CH3
UDP + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)(Xylbeta1-2)Manbeta1-O-(CH2)7-CH3
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?
UDP-D-xylose + glucuronoxylomannan
UDP + [beta-D-xylose-(1,2)]-glucuronoxylomannan
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JEC21/cxt1delta and CAP67/cxt1delta lacking CXT1p: 30% decrease in amount of xylose, 39% decrease in O-2 xylose-substituted mannose, 31.5% increase in O-2-unsubstituted mannose, loss of 1/3 of beta-(1,2)-linked xylose, revealed by NMR analyses
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?
UDP-D-xylose + glucuronoxylomannan
UDP + [beta-D-xylosyl-(1,2)]-glucuronoxylomannan
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?
UDP-D-xylose + Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc1-O-(N-benzyloxycarbonyl)-Tyr
UDP + Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)(Xylbeta1-2)Manbeta1-4GlcNAcbeta1-4GlcNAc1-O-(N-benzyloxycarbonyl)-Tyr
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?
UDP-D-xylose + Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-O-(CH2)7-CH3
UDP + Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)(Xylbeta1-2)Manbeta1-O-(CH2)7-CH3
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?
UDP-D-xylose + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine
UDP + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-[beta-D-xylosyl-(1,2)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine
UDP-D-xylose + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine
UDP + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-[beta-D-xylosyl-(1->2)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine
UDP-xylose + dansylated Galbeta-(1,4)-GlcNAcbeta-(1,2)-Manalpha-(1,6)-(Galbeta-(1,4)-GlcNAcbeta-(1,2)-Manalpha-(1,3))-Manbeta-(1,4)-GlcNAcbeta-(1,4)-GlcNAc
UDP + ?
UDP-xylose + mannosyl-alpha-(1,3)-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide
UDP + mannosyl-alpha-(1,3)-[xylosyl-beta-(1,2)]-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide
no transfer of xylose residues onto substrate in absence of Cxt1p (JEC21/cxt1delta) as revealed by mass spectrometry and HPTLC, mannosyl-alpha-(1,3)-mannose-alpha motif also present in substrates for capsule polysaccharide synthesis
core structure of glycosylinositol phosphorylceramides, GIPCs
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?
UDP-xylose + mannosyl-alpha-(1,6)-mannosyl-alpha-(1,3)-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide
UDP + mannosyl-alpha-(1,6)-mannosyl-alpha-(1,3)-[xylosyl-beta-(1,2)]-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide
no transfer of xylose residues onto substrate in absence of Cxt1p (JEC21/cxt1delta) as revealed by mass spectrometry and HPTLC
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?
Xylbeta1-2[Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)]Manbeta1-O-octyl + UDP-xylose
?
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i.e. MGnX-octyl
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-
?
Xylbeta1-2[Manalpha1-6(Manalpha1-3)]Manbeta1-O-octyl + UDP-xylose
?
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i.e. MMX-octyl
-
-
?
[GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-3)GlcNAc]-GP + UDP-xylose
?
-
i.e. GnGnF
-
-
?
[Manalpha1-6(Manalpha1-3)Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc]-GP + UDP-xylose
?
-
i.e. Man5Gn
-
-
?
UDP-D-xylose + D-mannosyl-alpha-(1,3)-D-mannose
UDP + D-xylosyl-beta-(1,2)-D-mannosyl-alpha-(1,3)-D-mannose
membranes or purified enzyme, radiolabelled UDP-xylose, 8.5-12.5 mM alpha-(1,3)-mannobiose (resembles backbone or dimannose moiety of natural substrates), pH 6.5, 4 h, 20°C
product: trisaccharide with xylose moiety linked to reducing mannose of alpha-(1,3)-mannobiose as revealed by mass spectrometry, NMR and methylation linkage analyses, radiolabel detection by scintillation counting or thin layer chromatography and autoradiography or product purification by solid phase extraction
-
?
UDP-D-xylose + D-mannosyl-alpha-(1,3)-D-mannose
UDP + D-xylosyl-beta-(1,2)-D-mannosyl-alpha-(1,3)-D-mannose
membranes, radiolabelled UDP-xylose, alpha-(1,3)-mannobiose (resembles backbone or dimannose moiety of natural substrates)
product: trisaccharide with xylose moiety linked to reducing mannose of alpha-(1,3)-mannobiose, radiolabel detection by scintillation counting or thin layer chromatography and autoradiography, product purification for NMR and glycosyl linkage analyses
-
?
UDP-D-xylose + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc1-O-(pyrid-2-yl)amine
UDP + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)(Xylbeta1-2)Manbeta1-4GlcNAcbeta1-4GlcNAc1-O-(pyrid-2-yl)amine
-
-
-
?
UDP-D-xylose + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc1-O-(pyrid-2-yl)amine
UDP + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)(Xylbeta1-2)Manbeta1-4GlcNAcbeta1-4GlcNAc1-O-(pyrid-2-yl)amine
-
-
?
UDP-D-xylose + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine
UDP + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-[beta-D-xylosyl-(1,2)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
-
-
-
?
UDP-D-xylose + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine
UDP + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-[beta-D-xylosyl-(1,2)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
-
-
?
UDP-D-xylose + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine
UDP + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-[beta-D-xylosyl-(1->2)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
-
-
?
UDP-D-xylose + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine
UDP + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-[beta-D-xylosyl-(1->2)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
-
-
-
?
UDP-D-xylose + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine
UDP + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-[beta-D-xylosyl-(1->2)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
-
-
-
?
UDP-xylose + dansylated Galbeta-(1,4)-GlcNAcbeta-(1,2)-Manalpha-(1,6)-(Galbeta-(1,4)-GlcNAcbeta-(1,2)-Manalpha-(1,3))-Manbeta-(1,4)-GlcNAcbeta-(1,4)-GlcNAc
UDP + ?
-
i.e. dansylated GnGn-peptide
-
-
?
UDP-xylose + dansylated Galbeta-(1,4)-GlcNAcbeta-(1,2)-Manalpha-(1,6)-(Galbeta-(1,4)-GlcNAcbeta-(1,2)-Manalpha-(1,3))-Manbeta-(1,4)-GlcNAcbeta-(1,4)-GlcNAc
UDP + ?
i.e. dansylated GnGn-peptide
-
-
?
additional information
?
-
substrate specificity, substrates need to possess a beta(1,2)-linked GlcNAc residue at the Man-alpha-(1,3) arm, Man-alpha-(1,6)-(Man-alpha(1,3))-Man-beta(1-R) is no acceptor substrate, integrity of a large domain located between amino acid 31 and the C-terminal lumenal region is required for activity, the cytosolic tail and the transmembrane region are not essential for activity, but the stem region and the C-terminal end are
-
-
?
additional information
?
-
-
substrate specificity, substrates need to possess a beta(1,2)-linked GlcNAc residue at the Man-alpha-(1,3) arm, Man-alpha-(1,6)-(Man-alpha(1,3))-Man-beta(1-R) is no acceptor substrate, integrity of a large domain located between amino acid 31 and the C-terminal lumenal region is required for activity, the cytosolic tail and the transmembrane region are not essential for activity, but the stem region and the C-terminal end are
-
-
?
additional information
?
-
-
transfer of D-xylose from UDP-D-xylose to the core beta-linked mannose of N-linked oligosaccharides
-
-
?
additional information
?
-
-
the enzyme is involved in the biosynthesis of glycoprotein-bound N-glycans in plants and acts at multiple stages of the plant N-glycosylation pathway, overview
-
-
?
additional information
?
-
-
the enzyme is specific for UDP-xylose, no activity with Man5-GP and MM-octyl, acceptor substrate specificity, overview
-
-
?
additional information
?
-
preferred substrate depends on wild-type strain, strain JEC21: both products detectable (mannosyl-alpha-(1,6)-mannosyl-alpha-(1,3)-[xylosyl-beta-(1,2)]-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide and mannosyl-alpha-(1,3)-[xylosyl-beta-(1,2)]-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide) at a ratio of approximately 1:1
-
-
?
additional information
?
-
-
preferred substrate depends on wild-type strain, strain JEC21: both products detectable (mannosyl-alpha-(1,6)-mannosyl-alpha-(1,3)-[xylosyl-beta-(1,2)]-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide and mannosyl-alpha-(1,3)-[xylosyl-beta-(1,2)]-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide) at a ratio of approximately 1:1
-
-
?
additional information
?
-
-
transfer of beta (1,2)-xylose, capsule synthesis
-
-
?
additional information
?
-
transfer of beta (1,2)-xylose, capsule synthesis
-
-
?
additional information
?
-
transfer of beta (1,2)-xylose, synthesis of capsule polysaccharides and fungi-specific glycosphingolipids
-
-
?
additional information
?
-
-
transfer of beta (1,2)-xylose, synthesis of capsule polysaccharides and fungi-specific glycosphingolipids
-
-
?
additional information
?
-
-
lack of CXT1p in membranes from JEC21/cxt1delta and CAP67/cxt1delta leads to dramatic reduction in D-xylose-beta-(1,2)-D-mannose-alpha-(1,3)-D-mannose synthesising activity in presence of exogenously added substrate
-
-
?
additional information
?
-
lack of CXT1p in membranes from JEC21/cxt1delta and CAP67/cxt1delta leads to dramatic reduction in D-xylose-beta-(1,2)-D-mannose-alpha-(1,3)-D-mannose synthesising activity in presence of exogenously added substrate
-
-
?
additional information
?
-
-
probably involved in the biosynthesis of the inner part of hemocyanin glycans
-
-
?
additional information
?
-
-
-
strongly reduced biosynthesis of beta 1,2-xylose-containing glycoepitopes upon knock-down of endogenous beta 1,2-xylosyltransferase using sense, antisense and RNA interference approaches
-
?
additional information
?
-
-
-
strongly reduced biosynthesis of beta 1,2-xylose-containing glycoepitopes upon knock-down of endogenous beta 1,2-xylosyltransferase using RNA interference approaches
-
?
additional information
?
-
-
-
human anti HIV monoclonal antibody 2G12 produced in absence of endogenous beta 1,2-xylosyltransferase (knock-down by RNA interference) is functional but contains N-glycan species lacking beta 1,2-xylose residues corresponding to authentic human N-glycosylation
-
?
additional information
?
-
enzyme is involved in N-glycan biosynthesis, quantification and structure analysis of N-glycans in rice leaves
-
-
?
additional information
?
-
-
enzyme is involved in N-glycan biosynthesis, quantification and structure analysis of N-glycans in rice leaves
-
-
?
additional information
?
-
the enzyme is unable to add xylosyl residues onto the arabinosyl side chains of xylan
-
-
-
additional information
?
-
the enzyme is involved in the modification of asparagine-linked oligosaccharides of maize glycoproteins with beta1,2-xylose sugar residues
-
-
?
additional information
?
-
-
the enzyme is involved in the modification of asparagine-linked oligosaccharides of maize glycoproteins with beta1,2-xylose sugar residues
-
-
?
additional information
?
-
the recombinant enzyme is able to convert a mammalian acceptor glycoprotein into an antigen binding anti-plant N-glycan antibodies, overview
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-xylose + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein]
UDP + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-[beta-D-Xyl-(1->2)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein]
-
-
-
-
?
UDP-alpha-D-xylose + xylan
?
the enzyme adds side chain xylosyl residues directly to the xylan backbone at 2-O
-
-
?
UDP-D-xylose + galactoxylomannan
UDP + [beta-D-xylose-(1,2)]-galactoxylomannan
-
JEC21/cxt1delta and CAP67/cxt1delta lacking CXT1p: almost complete loss of beta-xylose residues (1,2)-linked to mannose, unchanged amounts of beta-xylose (1,3)-linked to galactose
-
?
UDP-D-xylose + galactoxylomannan
UDP + [beta-D-xylosyl-(1,2)]-galactoxylomannan
-
-
-
?
UDP-D-xylose + glucuronoxylomannan
UDP + [beta-D-xylose-(1,2)]-glucuronoxylomannan
-
JEC21/cxt1delta and CAP67/cxt1delta lacking CXT1p: 30% decrease in amount of xylose, 39% decrease in O-2 xylose-substituted mannose, 31.5% increase in O-2-unsubstituted mannose, loss of 1/3 of beta-(1,2)-linked xylose, revealed by NMR analyses
-
?
UDP-D-xylose + glucuronoxylomannan
UDP + [beta-D-xylosyl-(1,2)]-glucuronoxylomannan
-
-
-
?
UDP-D-xylose + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine
UDP + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-[beta-D-xylosyl-(1,2)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine
UDP-D-xylose + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine
UDP + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-[beta-D-xylosyl-(1->2)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine
UDP-xylose + mannosyl-alpha-(1,3)-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide
UDP + mannosyl-alpha-(1,3)-[xylosyl-beta-(1,2)]-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide
no transfer of xylose residues onto substrate in absence of Cxt1p (JEC21/cxt1delta) as revealed by mass spectrometry and HPTLC, mannosyl-alpha-(1,3)-mannose-alpha motif also present in substrates for capsule polysaccharide synthesis
core structure of glycosylinositol phosphorylceramides, GIPCs
-
?
UDP-xylose + mannosyl-alpha-(1,6)-mannosyl-alpha-(1,3)-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide
UDP + mannosyl-alpha-(1,6)-mannosyl-alpha-(1,3)-[xylosyl-beta-(1,2)]-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide
no transfer of xylose residues onto substrate in absence of Cxt1p (JEC21/cxt1delta) as revealed by mass spectrometry and HPTLC
-
-
?
UDP-D-xylose + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine
UDP + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-[beta-D-xylosyl-(1,2)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
-
-
-
?
UDP-D-xylose + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine
UDP + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-[beta-D-xylosyl-(1,2)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
-
-
?
UDP-D-xylose + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine
UDP + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-[beta-D-xylosyl-(1->2)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
-
-
?
UDP-D-xylose + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine
UDP + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-[beta-D-xylosyl-(1->2)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
-
-
-
?
UDP-D-xylose + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine
UDP + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-[beta-D-xylosyl-(1->2)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
-
-
-
?
additional information
?
-
-
the enzyme is involved in the biosynthesis of glycoprotein-bound N-glycans in plants and acts at multiple stages of the plant N-glycosylation pathway, overview
-
-
?
additional information
?
-
preferred substrate depends on wild-type strain, strain JEC21: both products detectable (mannosyl-alpha-(1,6)-mannosyl-alpha-(1,3)-[xylosyl-beta-(1,2)]-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide and mannosyl-alpha-(1,3)-[xylosyl-beta-(1,2)]-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide) at a ratio of approximately 1:1
-
-
?
additional information
?
-
-
preferred substrate depends on wild-type strain, strain JEC21: both products detectable (mannosyl-alpha-(1,6)-mannosyl-alpha-(1,3)-[xylosyl-beta-(1,2)]-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide and mannosyl-alpha-(1,3)-[xylosyl-beta-(1,2)]-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide) at a ratio of approximately 1:1
-
-
?
additional information
?
-
-
transfer of beta (1,2)-xylose, capsule synthesis
-
-
?
additional information
?
-
transfer of beta (1,2)-xylose, capsule synthesis
-
-
?
additional information
?
-
transfer of beta (1,2)-xylose, synthesis of capsule polysaccharides and fungi-specific glycosphingolipids
-
-
?
additional information
?
-
-
transfer of beta (1,2)-xylose, synthesis of capsule polysaccharides and fungi-specific glycosphingolipids
-
-
?
additional information
?
-
-
probably involved in the biosynthesis of the inner part of hemocyanin glycans
-
-
?
additional information
?
-
-
-
strongly reduced biosynthesis of beta 1,2-xylose-containing glycoepitopes upon knock-down of endogenous beta 1,2-xylosyltransferase using sense, antisense and RNA interference approaches
-
?
additional information
?
-
-
-
strongly reduced biosynthesis of beta 1,2-xylose-containing glycoepitopes upon knock-down of endogenous beta 1,2-xylosyltransferase using RNA interference approaches
-
?
additional information
?
-
enzyme is involved in N-glycan biosynthesis, quantification and structure analysis of N-glycans in rice leaves
-
-
?
additional information
?
-
-
enzyme is involved in N-glycan biosynthesis, quantification and structure analysis of N-glycans in rice leaves
-
-
?
additional information
?
-
the enzyme is unable to add xylosyl residues onto the arabinosyl side chains of xylan
-
-
-
additional information
?
-
the enzyme is involved in the modification of asparagine-linked oligosaccharides of maize glycoproteins with beta1,2-xylose sugar residues
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Mn2+
additional information
additional information
activity not dependent on divalent cations
additional information
is active in the presence of 10 mM EDTA and does not require the addition of any metal ions for activity
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
10 mM EDTA does not reduce endogenous activity
-
additional information
10 mM EDTA does not reduce endogenous activity
-
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Adenoma, Pleomorphic
Effect of Xylosyltransferase-I Silencing on Implanting Growth of Salivary Pleomorphic Adenoma.
Aortic Aneurysm, Abdominal
Xylosyltransferase I variants and their impact on abdominal aortic aneurysms.
Connective Tissue Diseases
Measurement of fibrosis marker xylosyltransferase I activity by HPLC electrospray ionization tandem mass spectrometry.
Diabetes Mellitus, Type 1
The Xylosyltransferase I Gene Polymorphism c.343G>T (p.A125S) Is a Risk Factor for Diabetic Nephropathy in Type 1 Diabetes.
Diabetic Nephropathies
Novel sequence variants in the human xylosyltransferase I gene and their role in diabetic nephropathy.
Diabetic Nephropathies
The Xylosyltransferase I Gene Polymorphism c.343G>T (p.A125S) Is a Risk Factor for Diabetic Nephropathy in Type 1 Diabetes.
Essential Hypertension
Xylosyltransferase gene variants and their role in essential hypertension.
Mucopolysaccharidosis III
Impairment of glycosaminoglycan synthesis in mucopolysaccharidosis type IIIA cells by using siRNA: a potential therapeutic approach for Sanfilippo disease.
Myopia
Mutations in DZIP1 and XYLT1 are associated with nonsyndromic early onset high myopia in the Korean population.
Neoplasm Metastasis
The effect of proteoglycans inhibited by RNA interference on metastatic characters of human salivary adenoid cystic carcinoma.
Obesity
An epigenome-wide study of body mass index and DNA methylation in blood using participants from the Sister Study cohort.
Osteoarthritis
Xylosyltransferase-I regulates glycosaminoglycan synthesis during the pathogenic process of human osteoarthritis.
Pseudoxanthoma Elasticum
Elevated xylosyltransferase I activities in pseudoxanthoma elasticum (PXE) patients as a marker of stimulated proteoglycan biosynthesis.
Renal Insufficiency
Serum xylosyltransferase I activity, the new biochemical fibrosis marker, is not affected by renal insufficiency.
Scleroderma, Systemic
Cytokine-mediated induction of human xylosyltransferase-I in systemic sclerosis skin fibroblasts.
Scleroderma, Systemic
Serum xylosyltransferase I activity, the new biochemical fibrosis marker, is not affected by renal insufficiency.
Spinal Cord Injuries
Transcriptional regulation of scar gene expression in primary astrocytes.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.055
dansylated Galbeta-(1,4)-GlcNAcbeta-(1,2)-Manalpha-(1,6)-(Galbeta-(1,4)-GlcNAcbeta-(1,2)-Manalpha-(1,3))-Manbeta-(1,4)-GlcNAcbeta-(1,4)-GlcNAc
recombinant soluble enzyme, pH 6.0, 16°C
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
transfer of 300 cpm/min/mg by endogenous activity in crude membranes
additional information
transfer of 300 cpm/min/mg by endogenous activity in crude membranes
additional information
-
transfer of 890000 cpm/min/mg by endogenous, purified activity after alpha-(1,3)-mannobiose affinity chromatography
additional information
transfer of 890000 cpm/min/mg by endogenous, purified activity after alpha-(1,3)-mannobiose affinity chromatography
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
16
37
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
serotype D, strain JEC21 (serotype D MATalpha) and strain ags1delta (serotype D MATalpha ags1delta)
UniProt
brenda
serotype D, wild-type strain JEC21 (serotype D MATalpha) and mutant strain JEC21/cxt1delta (serotype D, isogenic MAT alpha, encapsulated and nourseothricin-resistant, lacking CXT1p)
UniProt
brenda
wild-type strain JEC20 (serotype D MAT a), wild-type strain JEC21 (serotype D MAT alpha), mutant strain CAP67 (serotype D MAT alpha cap59), mutant strain JEC21/cxt1delta (serotype D, isogenic MAT alpha, encapsulated and nourseothricin-resistant, lacking CXT1p), mutant strain CAP67/cxt1delta (serotype D, isogenic MAT alpha, acapsulated and nourseothricin-resistant, lacking CXT1p)
-
-
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
the CTS region, formed by the N-terminal 90 amino acids, is responsible for correct targeting of the enzyme to the cytosplamic membrane
brenda
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
metabolism
-
the enzyme catalyzes the initial step of glycosaminoglycan biosynthesis
physiological function
Xt1p has a defined role in capsule biosynthesis
malfunction
loss of XT1 does not affect in vitro growth of mutant cells or general morphology of their capsules. The two main capsule polysaccharides, glucuronoxylomannan and galactoxylomannan, both are missing beta1,2-xylose residues. Deletion of XT1 leads to attenuation of cryptococcal growth in a mouse model of infection
malfunction
-
expression of the tomato beta1,2-xylosyltransferase is down-regulated by RNA interference in transgenic tomato plants. On the basis of IgE immunoblotting assays XylT-RNAi tomato fruits reveal a patient-specific reduction in IgE reactivity. Patients with tomato allergy have reduced mean wheal diameters in skin prick tests, demonstrating a reduced allergenic potential of XylT-RNAi tomato fruits in vivo
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). XYLT_ARATH
534
1
60235
Swiss-Prot
Secretory Pathway (Reliability: 1)
A0A8B6BWT6_MYTGA
458
0
53574
TrEMBL
other Location (Reliability: 3)
D8LR02_ECTSI
614
1
68197
TrEMBL
Mitochondrion (Reliability: 2)
A0A6J8A1J2_MYTCO
451
0
53167
TrEMBL
other Location (Reliability: 3)
Q0VH30_MAIZE
315
0
35411
TrEMBL
other Location (Reliability: 2)
A0A2P6QMQ0_ROSCH
240
0
27179
TrEMBL
other Location (Reliability: 2)
A0A812ARK0_SEPPH
591
1
66051
TrEMBL
Secretory Pathway (Reliability: 5)
A0A8B6BWN7_MYTGA
478
1
56039
TrEMBL
Secretory Pathway (Reliability: 3)
A0A0B2R2U6_GLYSO
371
0
41496
TrEMBL
other Location (Reliability: 2)
A0A7C9A790_OPUST
142
0
15812
TrEMBL
other Location (Reliability: 4)
G7J1G3_MEDTR
509
1
57395
TrEMBL
Secretory Pathway (Reliability: 1)
B9SSR0_RICCO
499
1
57069
TrEMBL
Secretory Pathway (Reliability: 1)
A0A8B6FH64_MYTGA
478
1
56041
TrEMBL
Secretory Pathway (Reliability: 4)
A0A0B2R424_GLYSO
359
0
40582
TrEMBL
other Location (Reliability: 2)
A0A6J8EKJ9_MYTCO
440
1
51802
TrEMBL
Secretory Pathway (Reliability: 2)
A0A2P6QD03_ROSCH
329
0
37312
TrEMBL
Secretory Pathway (Reliability: 1)
A0A6J8AG70_MYTCO
423
0
49531
TrEMBL
Mitochondrion (Reliability: 2)
A0A6J8EC55_MYTCO
554
0
64613
TrEMBL
other Location (Reliability: 1)
Q599J2_POPCN
468
0
53208
TrEMBL
Secretory Pathway (Reliability: 1)
A0A8B6G5F0_MYTGA
524
1
60838
TrEMBL
Secretory Pathway (Reliability: 1)
C1LGM8_SCHJA
453
1
52840
TrEMBL
Secretory Pathway (Reliability: 2)
Q0PMD1_LEMMI
509
1
57583
TrEMBL
Mitochondrion (Reliability: 2)
A0A8B6BEU9_MYTGA
496
0
57927
TrEMBL
other Location (Reliability: 2)
A0A2P6QCZ5_ROSCH
507
1
57162
TrEMBL
Secretory Pathway (Reliability: 1)
A0A7C9A4Z3_OPUST
110
0
12140
TrEMBL
other Location (Reliability: 4)
C1LGM7_SCHJA
229
2
26824
TrEMBL
Secretory Pathway (Reliability: 2)
Q2UVB6_SORBI
453
0
49893
TrEMBL
Mitochondrion (Reliability: 5)
Q2UVB7_HORVU
508
1
56199
TrEMBL
Mitochondrion (Reliability: 1)
Q5TIN6_SACOF
515
1
56773
TrEMBL
Mitochondrion (Reliability: 1)
A0A2I0A4K8_9ASPA
519
1
57453
TrEMBL
Mitochondrion (Reliability: 2)
Q2UVB4_VITVI
470
0
53014
TrEMBL
Chloroplast (Reliability: 5)
A0A6J8BJS6_MYTCO
257
0
30174
TrEMBL
other Location (Reliability: 5)
A0A8B6CQN2_MYTGA
440
1
51553
TrEMBL
Secretory Pathway (Reliability: 1)
A0A812E8V4_SEPPH
1597
39
195057
TrEMBL
other Location (Reliability: 5)
Q2UVB5_SOLTU
522
0
58829
TrEMBL
Secretory Pathway (Reliability: 2)
A0A8B6FIA1_MYTGA
458
0
53560
TrEMBL
other Location (Reliability: 3)
A0A2G9G953_9LAMI
509
0
57388
TrEMBL
Secretory Pathway (Reliability: 1)
A0A6J8EW79_MYTCO
458
0
53543
TrEMBL
Mitochondrion (Reliability: 4)
A0A5B7CA63_DAVIN
533
1
60573
TrEMBL
Mitochondrion (Reliability: 3)
A0A8B6BSL0_MYTGA
410
0
48250
TrEMBL
Secretory Pathway (Reliability: 2)
A0A2P6QMH9_ROSCH
126
0
14353
TrEMBL
other Location (Reliability: 4)
CXT1_CRYNJ
Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)
694
0
78970
Swiss-Prot
-
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
79000
90000
90000
hexa-His tagged wild-type and mutants D550A and D659A, 12% SDS-PAGE
90000
purified endogenous activity (serotype D), 12% SDS-PAGE and S-300 gel filtration, identity confirmed by mass spectrometry
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
primary sequence and hydrophobicity analysis of recombinant XylT
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
proteolytic modification
-
proteolytic processing of recombinant XylT occurs between amino acids Arg178 and Arg179, a cleavage sequence compatible with the enzymatic properties of the baculoviral cysteine proteinase
glycoprotein
3 potential N-glycosylation sites, Asn51, Asn301, and Asn478, 2 of which are glycosylated, i.e. Asn51 and Asn301, occupancy of at least one site is necessary for enzyme activity and stability, glycosylation sites of diverse mutants, overview
glycoprotein
-
three potential N-glycosylation sites of the wild-type enzyme as well as of the recombinant enzyme are occupied by N-linked oligosaccharides, N-glycosylation is not essential for proper folding of XylT
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
S53A
site-directed mutagenesis, possesses the N-glycosylation sites Asn301 and Asn478
T303A
site-directed mutagenesis, possesses the N-glycosylation sites Asn51 and Asn478
T480A
site-directed mutagenesis, possesses the N-glycosylation sites Asn51 and Asn301
D550A
mutation of first DXD motif to AXD, complete loss of activity, expression in Saccharomyces cerevisiae
D659A
mutation of second DXD motif to AXD, dramatic reduction in activity, expression in Saccharomyces cerevisiae
N141Q
mutation of the N-glycosylation site, molecular weight shift to 79 kDa corresponding to the molecular weight of the unmodified enzyme, no change in activity, expression in Saccharomyces cerevisiae
additional information
additional information
-
construction of transgenic Nicotiana benthamiana plants expressing the CTSregion, T and C region, and a membrane-shed enzyme version, the latter is secreted to the apoplast, the T or C region constructs are located in the cytoplasm, the CTS construct is located in the cytoplasmic membrane, overview
additional information
serial deletions at both the N- and the C-terminal ends, integrity of a large domain located between amino acid 31 and the C-terminal lumenal region is required for activity, construction of transgenic tobacco expressing the enzyme via Agrobacterium tumefaciens infection system
additional information
-
serial deletions at both the N- and the C-terminal ends, integrity of a large domain located between amino acid 31 and the C-terminal lumenal region is required for activity, construction of transgenic tobacco expressing the enzyme via Agrobacterium tumefaciens infection system
additional information
-
construction of several N-terminal deletion mutants, the first 54 residues can be deleted without affecting the catalytic activity of the enzyme, removal of an additional five amino acids lead to the formation of an inactive protein
additional information
co-expression of human monoclonal antibodies with specific RNAi to block beta-1,2-xylosyltransferase and alpha-1,3-fucosyltransferase prevents unwanted plant specific N-glycosylation of the recombinant antibody proteins, method optimization, overview
additional information
-
co-expression of human monoclonal antibodies with specific RNAi to block beta-1,2-xylosyltransferase and alpha-1,3-fucosyltransferase prevents unwanted plant specific N-glycosylation of the recombinant antibody proteins, method optimization, overview
additional information
targeted downregulation of the enzyme and the alpha1,3-fucosyltransferase allow production of heterologous proteins in a maize expression system
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
the purified enzyme is stable for prolonged periods at neutral pH and at temperatures of up to 30°C
671831
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
-
the purified enzyme is stable for prolonged periods at neutral pH and at temperatures of up to 30°C
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
the purified recombinant enzyme is not stable to a single freeze-thaw cycle
-
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C to -20° C, HEPES-buffer, pH 7.0, 10% glycerol, 0.2% Triton X-100, 1 mM dithiothreitol, 90 days, 20%
-
4C, purified recombinant enzyme, 3 weeks stable without loss of activity
4C, purified recombinant enzyme, 3 weeks stable without loss of activity
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
endogenous activity (2967-fold purification) from serotype D ags1delta strain: solubilisation from crude membranes with 1% Triton X-100 (on ice, 30 min), HiPrep 16/10 Q-Sepharose chromatography with NaCl gradient elution (pH 8), activity peaks were pooled and concentrated (spin, 10000 Da cut-off), HiPrep 26/60 Sephacryl S-300 gel filtration chromatography with isocratic gradient elution (pH 8), concanavalin A-Sepharose 4B chromatography with alpha-methyl-mannoside elution (incubation over night, 4°C), HiTrap DEAE chromatorgraphy with NaCl gradient elution (pH 8), alpha-(1,3)-mannobiose agarose resin affinity chromatography with NaCl gradient (pH 6.5), activity peaks were combined and concentrated, overexpressed hexa-His tagged enzyme: solubilisation, Talon Superflow cobalt metal affinity chromatography, elution with 250 mM imidazole, pH 7.5
recombinant N-terminally His5-tagged enzyme from Pichia pastoris cell medium by nickel chelating affinity chromatography and ultrafiltration to homogeneity
-
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
5-kb PCR product cloned into the TOPO pCR2.1 vector and maintained in Escherichia coli DH5alpha
amplification of the gene with flanking regions (1.3 kb upstream of the start codon, 1 kb downstream of the gene) from serotype D (strain JEC21) genomic DNA for cloning in pCR2.1TOPO, generation of replacement cassettes for replacing endogenous CXT1p: (i) nourseothricin acetyltransferase resistance gene flanked by the CXT1p flanking regions, transformed as linear DNA in CAP67 strain, (ii) cxt1 gene lacking 3 two-thirds of the encoding sequence flanked by its flanking regions, transformed as linear DNA in JEC21 strain
DNA and amino acid sequence determination and analysis, expression of full length wild-type enzyme and diverse mutants in Spodoptera frugiperda Sf9 insect cells via baculovirus infection system as soluble proteins lacking the transmembrane domain, expression of the full length enzyme and enzyme fragments as GFP-fusion proteins in tobacco cells Golgi medial cisternae via Agrobacterium tumefaciens infection system for study of enzyme subcellular localization
DNA and amino acid sequence determination and analysis, gene is located on chromosome 8, functional expression of the enzyme in Pichia pastoris as soluble protein, secretion to the cell culture medium
DNA and amino acid sequence determination and analysis, genetic organization and structure, functional expression in Spodoptera frugiperda Sf21 cells using the baculovirus transfection system
expressed in Arabidopsis thaliana gux1/2/3 triple mutant and HEK-293F cells
expression of N-terminally His5-tagged enzyme in Pichia pastoris, recombinant enzyme is secreted to the medium
-
expression of the enzyme's isolated CTS region, N-terminal 90 amino acids, and of the T and C region separately, as fusion protein with GFP in Nicotiana benthamiana leaf epidermal cells, the CT region, but not only the T or C regions, is sufficient to sustain Golgi retention without the involvement of any luminal sequences
-
expression of wild-type and mutant enzymes in Spodoptera frugiperda SF21 cells using the baculovirus infection system
-
from JEC21 cDNA by PCR with primers introducing C-terminal hexa-His tag, in pCR2.1 TOPO for sequencing, in pPGK (cloned between promoter and terminator of phosphoglycerate kinase) for expression in Saccharomyces cerevisiae that lacks endogenous xylosyltransferase activity
gene xylt, transfection of chimeric constructs via Agrobacterium tumefaciens strain C58Z707, overview
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
isoform XYLT1 expression is inhibited by transforming growth factor-beta receptor I inhibitor SB431542
-
mannitol treatment up-regulates enzyme expression in shoots and roots, whereas treatment at 42°C up-regulates the expression in shoots only. Enzyme expression is up-regulated by application of absisic acid or jasmonic acid (shoots), and 1-aminocyclopropane-1-carboxylic acid, 6-benzylaminopurine, or epi-brassiolide (roots)
-
NaCl treatment reduces enzyme expression in roots. Enzyme expression is down-regulated by application of kinetin (shoots) and iodoacetic acid (roots)
-
treatment of normal human dermal fibroblasts with transforming growth factor-beta1 is followed by increased relative isoform XYLT1 mRNA expression. Moreover, enzyme activity increases time-dependently in response to progressive myofibroblast transformation
-
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
biotechnology
medicine
synthesis
potential of maize and the beta1,2-xylosyltransferase as a production system for heterologous glycoproteins
biotechnology
the plant is useful for expression of human monoclonal antibodies free of zoonotic pathogens, co-expression of RNAi to block beta-1,2-xylosyltransferase and alpha-1,3-fucosyltransferase prevents unwanted plant specific N-glycosylation of the recombinant antibody proteins, overview
biotechnology
-
glycoengineering: humanized N-glycosylation in plants by knock-down of plant-specific N-glycan maturation enzymes, pharmaceutical glycoprotein production in plant
biotechnology
-
pharmaceutical protein production in plant, glycoengineering: humanized N-glycosylation in plants by knock-down of plant-specific N-glycan maturation enzymes
medicine
development of diagnostic and therapeutic strategies due to importance of xylose transfer in cryptococcal biology, pathogenesis and virulence
medicine
xylose residues within the capsule polysaccharide are important for normal host-pathogen interaction, loss of CXT1p attenuates virulence
medicine
-
the enzyme is a biomarker for myofibroblast differentiation and fibrotic development
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mulder, H.; Dideberg, F.; Schachter, H.; Spronk, B.A.; De Jong-Brink, M.; Kamerling, J.P.; Vliegenthart, J.F.G.
In the biosynthesis of N-glycans in connective tissue of the snail Lymnaea stagnalis of incorporation GlcNAc by beta 2GlcNAc-transferase I is an essential prerequisite for the action of beta 2GlcNAc-transferase II and beta 2Xyl-transferase
Eur. J. Biochem.
232
272-283
1995
Lymnaea stagnalis
brenda
Tezuka, K.; Hayashi, M.; Ishihara, H.; Akazawa, T.; Takahashi, N.
Studies on synthetic pathway of xylose-containing N-linked oligosaccharides deduced from substrate specificities of the processing enzymes in sycamore cells (Acer pseudoplatanus L.)
Eur. J. Biochem.
203
401-413
1992
Acer pseudoplatanus
brenda
Strasser, R.; Mucha, J.; Mach, L.; Altmann, F.; Wilson, I.B.H.; Glossl, J.; Steinkellner, H.
Molecular cloning and functional expression of beta 1,2-xylosyltransferase cDNA from Arabidopsis thaliana
FEBS Lett.
472
105-108
2000
Arabidopsis thaliana (Q9LDH0), Arabidopsis thaliana
brenda
Zeng, Y.; Bannon, G.; Thomas, V.H.; Rice, K.; Drake, R.; Elbein, A.
Purification and specificity of beta 1,2-xylosyltransferase, an enzyme that contributes to the allergenicity of some plant proteins
J. Biol. Chem.
272
31340-31347
1997
Glycine max
brenda
Bencurova, M.; Rendic, D.; Fabini, G.; Kopecky, E.M.; Altmann, F.; Wilson, I.B.H.
Expression of eukaryotic glycosyltransferases in the yeast Pichia pastoris
Biochimie
85
413-422
2003
Arabidopsis thaliana
brenda
Pagny, S.; Bouissonnie, F.; Sarkar, M.; Follet-Gueye, M.L.; Driouich, A.; Schachter, H.; Faye, L.; Gomord, V.
Structural requirements for Arabidopsis beta1,2-xylosyltransferase activity and targeting to the Golgi
Plant J.
33
189-203
2003
no activity in Spodoptera frugiperda, Arabidopsis thaliana (Q9LDH0), Arabidopsis thaliana
brenda
Dirnberger, D.; Bencur, P.; Mach, L.; Steinkellner, H.
The Golgi localization of Arabidopsis thaliana beta1,2-xylosyltransferase in plant cells is dependent on its cytoplasmic and transmembrane sequences
Plant Mol. Biol.
50
273-281
2002
Arabidopsis thaliana
brenda
Leonard, R.; Kolarich, D.; Paschinger, K.; Altmann, F.; Wilson, I.B.H.
A genetic and structural analysis of the N-glycosylation capabilities of rice and other monocotyledons
Plant Mol. Biol.
55
631-644
2004
Oryza sativa (Q6ZFH6), Oryza sativa
brenda
Bencur, P.; Steinkellner, H.; Svoboda, B.; Mucha, J.; Strasser, R.; Kolarich, D.; Hann, S.; Koellensperger, G.; Gloessl, J.; Altmann, F.; Mach, L.
Arabidopsis thaliana beta1,2-xylosyltransferase: an unusual glycosyltransferase with the potential to act at multiple stages of the plant N-glycosylation pathway
Biochem. J.
388
515-525
2005
Arabidopsis thaliana
brenda
Cox, K.M.; Sterling, J.D.; Regan, J.T.; Gasdaska, J.R.; Frantz, K.K.; Peele, C.G.; Black, A.; Passmore, D.; Moldovan-Loomis, C.; Srinivasan, M.; Cuison, S.; Cardarelli, P.M.; Dickey, L.F.
Glycan optimization of a human monoclonal antibody in the aquatic plant Lemna minor
Nat. Biotechnol.
24
1591-1597
2006
Lemna minor (Q0PMC9), Lemna minor
brenda
Bondili, J.S.; Castilho, A.; Mach, L.; Gloessl, J.; Steinkellner, H.; Altmann, F.; Strasser, R.
Molecular cloning and heterologous expression of beta1,2-xylosyltransferase and core alpha1,3-fucosyltransferase from maize
Phytochemistry
67
2215-2224
2006
Zea mays (Q2UVB3), Zea mays
brenda
Castle, S.A.; Owuor, E.A.; Thompson, S.H.; Garnsey, M.R.; Klutts, J.S.; Doering, T.L.; Levery, S.B.
beta1,2-Xylosyltransferase Cxt1p is solely responsible for xylose incorporation into Cryptococcus neoformans glycosphingolipids
Eukaryot. Cell
7
1611-1615
2008
Cryptococcus neoformans (Q5K8R6), Cryptococcus neoformans
brenda
Klutts, J.S.; Levery, S.B.; Doering, T.L.
A beta-1,2-xylosyltransferase from Cryptococcus neoformans defines a new family of glycosyltransferases
J. Biol. Chem.
282
17890-17899
2007
Aspergillus clavatus, Aspergillus fischeri, Aspergillus fumigatus, Aspergillus nidulans, Aspergillus terreus, Chaetomium globosum, Coccidioides immitis, Cryptococcus neoformans, Cryptococcus neoformans (Q5K8R6), Fusarium graminearum, Pyricularia grisea, Neurospora crassa, Ustilago maydis
brenda
Klutts, J.S.; Doering, T.L.
Cryptococcal xylosyltransferase 1 (Cxt1p) from Cryptococcus neoformans plays a direct role in the synthesis of capsule polysaccharides
J. Biol. Chem.
283
14327-14334
2008
Cryptococcus neoformans, Cryptococcus neoformans (Q5K8R6)
brenda
Strasser, R.; Stadlmann, J.; Schaehs, M.; Stiegler, G.; Quendler, H.; Mach, L.; Gloessl, J.; Weterings, K.; Pabst, M.; Steinkellner, H.
Generation of glyco-engineered Nicotiana benthamiana for the production of monoclonal antibodies with a homogeneous human-like N-glycan structure
Plant Biotechnol. J.
6
392-402
2008
Nicotiana benthamiana
brenda
Sourrouille, C.; Marquet-Blouin, E.; DAoust, M.A.; Kiefer-Meyer, M.C.; Seveno, M.; Pagny-Salehabadi, S.; Bardor, M.; Durambur, G.; Lerouge, P.; Vezina, L.; Gomord, V.
Down-regulated expression of plant-specific glycoepitopes in alfalfa
Plant Biotechnol. J.
6
702-721
2008
Medicago sativa
brenda
Paulus, K.E.; Mahler, V.; Pabst, M.; Kogel, K.H.; Altmann, F.; Sonnewald, U.
Silencing beta1,2-xylosyltransferase in transgenic tomato fruits reveals xylose as constitutive component of IgE-binding epitopes
Front. Plant Sci.
2
42
2011
Solanum lycopersicum
brenda
Kajiura, H.; Okamoto, T.; Misaki, R.; Matsuura, Y.; Fujiyama, K.
Arabidopsis beta1,2-xylosyltransferase: substrate specificity and participation in the plant-specific N-glycosylation pathway
J. Biosci. Bioeng.
113
48-54
2012
Arabidopsis thaliana (Q9LDH0)
brenda
Matsuo, K.; Kagaya, U.; Itchoda, N.; Tabayashi, N.; Matsumura, T.
Deletion of plant-specific sugar residues in plant N-glycans by repression of GDP-D-mannose 4,6-dehydratase and beta-1,2-xylosyltransferase genes
J. Biosci. Bioeng.
118
448-454
2014
Nicotiana benthamiana
brenda
Faust, I.; Roch, C.; Kuhn, J.; Prante, C.; Knabbe, C.; Hendig, D.
Human xylosyltransferase-I - a new marker for myofibroblast differentiation in skin fibrosis
Biochem. Biophys. Res. Commun.
436
449-454
2013
Homo sapiens
brenda
Schoberer, J.; Liebminger, E.; Botchway, S.W.; Strasser, R.; Hawes, C.
Time-resolved fluorescence imaging reveals differential interactions of N-glycan processing enzymes across the Golgi stack in planta
Plant Physiol.
161
1737-1754
2013
Arabidopsis thaliana (Q9LDH0)
brenda
Takano, S.; Matsuda, S.; Funabiki, A.; Furukawa, J.; Yamauchi, T.; Tokuji, Y.; Nakazono, M.; Shinohara, Y.; Takamure, I.; Kato, K.
The rice RCN11 gene encodes beta1,2-xylosyltransferase and is required for plant responses to abiotic stresses and phytohormones
Plant Sci.
236
75-88
2015
Oryza sativa
brenda
Zeng, W.; Ford, K.L.; Bacic, A.; Heazlewood, J.L.
N-linked glycan micro-heterogeneity in glycoproteins of Arabidopsis
Mol. Cell. Proteomics
17
413-421
2018
Arabidopsis thaliana (Q9LDH0)
brenda
Jansing, J.; Sack, M.; Augustine, S.M.; Fischer, R.; Bortesi, L.
CRISPR/Cas9-mediated knockout of six glycosyltransferase genes in Nicotiana benthamiana for the production of recombinant proteins lacking beta-1,2-xylose and core alpha-1,3-fucose
Plant Biotechnol. J.
17
350-361
2019
Nicotiana benthamiana
brenda
Zhong, R.; Cui, D.; Phillips, D.R.; Ye, Z.H.
A novel rice xylosyltransferase catalyzes the addition of 2-O-xylosyl side chains onto the xylan backbone
Plant Cell Physiol.
59
554-565
2018
Oryza sativa Japonica Group (Q5Z8T8)
brenda
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