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2.4.1.69: type 1 galactoside alpha-(1,2)-fucosyltransferase

This is an abbreviated version!
For detailed information about type 1 galactoside alpha-(1,2)-fucosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.69

Reaction

GDP-beta-L-fucose
+
beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R
=
GDP
 +
alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R

Synonyms

a-(1->/2)-L-GalT, alpha (1,2)fucosyltransferase, alpha (1,2)fucosyltransferase 2, alpha (1,2)fucosyltransferase I, alpha 1, 2-fucosyltransferase 1, alpha(1,2)-fucosyltransferase, alpha(1,2)-fucosyltransferase-I, alpha(1,2)fucosyltransferase, alpha(1,2)fucosyltransferase I, alpha-(1,2)-fucosyltransferase, alpha-(1->/2)-L-galactosyltransferase, alpha-(1->2)-L-fucosyltransferase, alpha-1,2-fucosyltransferase, alpha-2-fucosyltransferase, alpha-2-L-fucosyltransferase, alpha-L-fucosyltransferase, alpha1,2-FT, alpha1,2-fucosyltransferase, alpha1,2-FucT, alpha1,2FT, alpha1,2fucosyltransferase, alpha1-2FucT, alpha1->2-fucosyltransferase, alpha1->2-FucT, alpha2-fucosyltransferase, alpha2FT, At2g03220, beta-galactoside alpha1-2fucosyltransferase, blood group H alpha-2-fucosyltransferase, blood-group substance H-dependent fucosyltransferase, CE2FT-2, EC 2.4.1.89, FTA, Ftb, fucosyltransferase, fucosyltransferase 1, fucosyltransferase 2, fucosyltransferase, guanosine diphosphofucose-galactoside 2-L-, fucosyltransferase, guanosine diphosphofucose-glycoprotein 2-alpha-, fucosyltransferase, guanosine diphosphofucose-lactose, FucT, FUT1, FUT2, FUT4, FUT4.1, FUT6, futC, galactoside 2-alpha-L-fucosyltransferase, galactoside 2-alpha-L-fucosyltransferase 1, galactoside 2-alpha-L-fucosyltransferase 2, GDP fucose-lactose fucosyltransferase, GDP-beta-L-fucose:beta-D-galactosyl-R 2-alpha-L-fucosyltransferase, GDP-fucose:GM1 alpha1-2fucosyltransferase, GDP-L-fucose:beta-D-galactosyl-R-alpha-L-fucosyltransferase, GDP-L-fucose:lactose fucosyltransferase, GDPFuc:beta-D-galactoside alpha1,2-fucosyltransferase, guanosine diphospho-L-fucose-lactose fucosyltransferase, guanosine diphosphofucose-beta-D-galactosyl-alpha-2-L-fucosyltransferase, guanosine diphosphofucose-galactosylacetylglucosaminylgalactosylglucosylceramide alpha-L-fucosyltransferase, guanosine diphosphofucose-glycoprotein 2-alpha-L-fucosyltransferase, H gene-encoded beta-galactoside alpha1->2 fucosyltransferase, H-gene-encoded beta-galactoside alpha1-2fucosyltransferase, MFUT-I, MFUT-II, pgtA, Se gene-encoded beta-galactoside alpha1->2 fucosyltransferase, Sec1, secretor-type beta-galactoside alpha1-2fucosyltransferase, WbgL, WbiQ, WbsJ, wbwK, WcfB

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.69 type 1 galactoside alpha-(1,2)-fucosyltransferase

Cloned

Cloned on EC 2.4.1.69 - type 1 galactoside alpha-(1,2)-fucosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of gene FTB and FTC
enzyme is stably transfected into Chinese hamster ovary cells
-
expressed as full-length protein with and without a C-terminal HPC4-epitope tag in human 293T cells
-
expressed as GST fusion protein in Escherichia coli BL21 (DE3)
Q5JBG3
expressed in COS-7 cells
-
expressed in Escherichia coli BL21(DE3) and JM109 (DE3) cells
expressed in Nicotiana tabacum BY2 cells
-
expressed in Sus scrofa
expression in COS-7 cells
-
expression in Escherichia coli
-
expression in NIH 3T3 cells
-
expression in Schizosaccharomyces pombe
expression of fut1, fut2, and sec1 genes in COS-7 cells
-
full length enzyme and truncated enzyme missing the first 27 amino acid residues from the N-terminus, comprising the cytoplasmic and transmembrane domains
porcine endothelial cell line transfected with human alpha(1,2)-fucosyltransferase cDNA
-
subcloned in CHO-K1 cells
-
subcloned in COS-7 or CHOP cells (Chinese hamster ovary cells transformed with Polyoma large T antigen)
-
the GST-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells
the transcription start site of FUT1 changes during differentiation of the erythroid lineage. The tissue-specific and stage-specific expression of the FUT1 are regulated by three distinct promoters. The 5'-flanking region of exon 2 consists of repetitive sequences. The start site of exon 2 is within the long terminal repeat. The repetitive sequences may play a role in the expression of the FUT1
-
the wbsJ gene is overexpressed in Escherichia coli BL21 as a fusion protein with glutathione S-transferase (GST) at its N-terminus
-
transfection of Chinese hamster ovary cells
transfection of the mouse Fut1 and Fut2, and human FUT1 genes into human ovarian carcinoma-derived RMG-1 cells results in 20–30fold increases in cellular alpha1,2-fucosyltransferase activity, and in alteration of the glycolipid composition, including not only fucosylated products, but also precursor glycolipids