2.4.1.227: undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase
This is an abbreviated version!
For detailed information about undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase, go to the full flat file.
Reaction
Synonyms
acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-acetylmuramoylpentapeptide pyrophospholipid, gene murG enzyme, gene murG proteins, HyMurG, Lipid I acetylglucosaminyltransferase, MsmegMurG, MurG, MurG glycosyltransferase, MurG transferase, peptidoglycan glycosyltransferase, proteins, gene murG, translocase II, UDP-acetylglucosamine-acetylmuramoylpentapeptide pyrophospholipid acetylglucosaminyltransferase
ECTree
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Cloned
Cloned on EC 2.4.1.227 - undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase
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expressed in Escherichia coli cells BL21-DE3-pLysS
gene murG, expression as His6-tagged enzyme in Escherichia coli strain C43
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gene murG, expression of C-terminal YFP-tagged enzyme in a murG-deficient strain of Bacillus subtilis
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into the vector pQE60 for expression of MurG in Escherichia coli JM109 cells
MurG can be displayed in its active form on phage. Cloned from a pET-21a expression vector (Novagen) into the pFAB5cHis.TT.HUI phagemid vector as a fusion to the 3-end of a pelB leader sequence and the 5-end of a truncated gIII
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the coding sequence is amplified from the Escherichia coli K12 chromosome and cloned for expression of His-tagged MurG in JM109 cells
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the murG gene is cloned with an N-terminal His6 tag into a pET33b vector. The plasmid is transformed and expressed in Escherichia coli NiCo21(DE3) competent cells
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the murG gene is cloned with an N-terminal His6 tag into a pET33b vector. The plasmid was transformed and expressed in Escherichia coli NiCo21(DE3) competent cells
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