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Literature summary for 2.4.1.227 extracted from
- The kinetik characterization of Escherichia coli MurG using synthetic substrate analogues (1999), J. Am. Chem. Soc., 121, 84158426.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Escherichia coli |  |
| Mg2+ | - |
Escherichia coli | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli cells BL21-DE3-pLysS | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Escherichia coli | |
| Ni2+ | - |
Escherichia coli | |
| Zn2+ | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.036 | - |
biotinylated citronellyl-lipid I | in the presence of Mn2+ | Escherichia coli | |
| 0.037 | - |
biotinylated citronellyl-lipid I | in the presence of Mg2+ | Escherichia coli | |
| 0.044 | - |
biotinylated citronellyl-lipid I | in the absence of additional metal ions | Escherichia coli | |
| 0.045 | - |
UDP-N-acetylglucosamine | in the absence of additional metal ions | Escherichia coli | |
| 0.046 | - |
UDP-N-acetylglucosamine | in the presence of Mn2+ | Escherichia coli | |
| 0.058 | - |
UDP-N-acetylglucosamine | in the presence of Mg2+ | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | cytoplasmic fraction | Escherichia coli | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | activating compound | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
2 * 38000, SDS-PAGE | Escherichia coli |
| 76000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
wild type | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for at least one month | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-alpha-D-glucosamine + biotinylated citronellyl-lipid I | - |
Escherichia coli | ? | - |
? | |
| UDP-N-acetylglucosamine + citronellyl-lipid I | synthetic substrate, 55-carbon undecaprenol chain replaced by the 10-carbon chain of citronellol, biotin labeled substrate | Escherichia coli | UDP + N-acetylglucosamine-citronellyl-lipid I | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 38000, SDS-PAGE | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.123 | - |
biotinylated citronellyl-lipid I | in the absence of additional metal ions | Escherichia coli | |
| 0.267 | - |
biotinylated citronellyl-lipid I | in the presence of Mg2+ | Escherichia coli | |
| 0.317 | - |
biotinylated citronellyl-lipid I | in the presence of Mn2+ | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
Hepes buffer | Escherichia coli |
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