2.4.1.227: undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase

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For detailed information about undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase, go to the full flat file.

Reaction

Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol

beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol

Synonyms

acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-acetylmuramoylpentapeptide pyrophospholipid, gene murG enzyme, gene murG proteins, HyMurG, Lipid I acetylglucosaminyltransferase, MsmegMurG, MurG, MurG glycosyltransferase, MurG transferase, peptidoglycan glycosyltransferase, proteins, gene murG, translocase II, UDP-acetylglucosamine-acetylmuramoylpentapeptide pyrophospholipid acetylglucosaminyltransferase

ECTree

2 Transferases

2.4 Glycosyltransferases

2.4.1 Hexosyltransferases

2.4.1.227 undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase

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Results	in table
9	Activating Compound
40181	AA Sequence
8	Application
1	CAS Registry Number
12	Cloned(Commentary)
2	Crystallization (Commentary)
18	General Information
1	General Stability
17	IC50 Value
71	Inhibitors
44	KM Value [mM]
20	Localization
2	Metals/Ions
8	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
33	Organism
9	Pathway
4	PDB ID
4	pH Optimum
1	pH Range
20	Protein Variants
9	Purification (Commentary)
2	Reaction
4	Reaction Type
32	Reference
23	Specific Activity [micromol/min/mg]
3	Storage Stability
45	Substrates and Products (Substrate)
6	Subunits
40	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
18	Turnover Number [1/s]

Purification

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Purification on EC 2.4.1.227 - undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase

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3 entries

native MurG is extracted from inner membrane vesicles of Escherichia coli cells, His-tagged MurG is purified by Ni-affinity chromatography using His-bind resin

Escherichia coli

676084

Ni2+ affinity column chromatography

Escherichia coli

703764

overexpression in Escherichia coli with His-tag

Escherichia coli

658448

recombinant His6-tagged enzyme from Escherichia coli strain C43 by nickel affinity chromatography

Escherichia coli

721824

using Ni2+-nitrilotriacetate-agarose

Escherichia coli

P17443

691120

when overexpressed, enzyme copurifies with lipid vesicles deriving from intracellular vesicular mebranes

Escherichia coli

659026