2.4.1.20: cellobiose phosphorylase

This is an abbreviated version!
For detailed information about cellobiose phosphorylase, go to the full flat file.

Word Map on EC 2.4.1.20

2.4.1.20

phosphorolysis

cellodextrins

phosphorylases

cellvibrio

gilvus

cellulolytic

thermocellum

cellulomonas

synthesis

alpha-d-glucose

laminaribiose

cellobiose-grown

cellotriose

alpha-d-glucose-1-phosphate

phosphorolyzed

6-deoxy-d-glucose

analysis

Reaction

Synonyms

Cbp, CbpA, cellobiose phosphorylase A, cellobiose:orthophosphate alpha-D-glucosyltransferase, CPase, CuCbP, GH94 cellobiose phosphorylase, lactose phosphorylase, ra2122, THA_1941, Tm_1848

ECTree

2 Transferases

2.4 Glycosyltransferases

2.4.1 Hexosyltransferases

2.4.1.20 cellobiose phosphorylase

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Results	in table
1	Activating Compound
146	AA Sequence
8	Application
1	CAS Registry Number
32	Cloned(Commentary)
3	Crystallization (Commentary)
2	Expression
2	General Information
3	General Stability
30	Inhibitors
58	kcat/KM [mM/s]
10	Ki Value [mM]
124	KM Value [mM]
7	Metals/Ions
19	Molecular Weight [Da]
49	Natural Substrates/ Products (Substrates)
6	Organic Solvent Stability
112	Organism
4	Pathway
28	PDB ID
19	pH Optimum
6	pH Range
3	pH Stability
26	Protein Variants
21	Purification (Commentary)
4	Reaction
1	Reaction Type
65	Reference
32	Specific Activity [micromol/min/mg]
205	Substrates and Products (Substrate)
18	Subunits
35	Synonyms
1	Systematic Name
14	Temperature Optimum [°C]
4	Temperature Range [°C]
15	Temperature Stability [°C]
91	Turnover Number [1/s]

Cloned

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Cloned on EC 2.4.1.20 - cellobiose phosphorylase

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2 entries

co-expressed with beta-glucanase GuxA in Caldicellulosiruptor bescii

Thermotoga maritima

751258

CP gene, expression of His-tagged wild-type and mutant enzymes in Escherichia coli

Cellulomonas uda

Q7WTR6

706588

expressed in Escherichia coli

3 entries

expressed in Escherichia coli BL21 (DE3)

Cellulomonas gilvus

672931

expressed in Escherichia coli BL21 cells

Cellulomonas uda

Q7WTR6

756244

expressed in Escherichia coli BL21(DE3) cells

3 entries

expressed in Escherichia coli BL21-CodonPlus(DE3) RIPL cells

Ruminococcus albus

758381

expressed in Escherichia coli JW0987 cells

Acetivibrio thermocellus

735417

expressed in Escherichia coli strain BL21 (DE3)

Acetivibrio thermocellus

674977

expressed in Escherichia coli, part of oligosaccharide catabolic cluster

Thermotoga neapolitana

O52504

637866

expressed in Saccharomyces cerevisiae strain D452-2

5 entries

expressed in Saccharomyces cerevisiae strain D452-2 and Escherichia coli BL21(DE3)

Saccharophagus degradans

735816

expression in Escherichia coli

4 entries

expression of a cellobiose phosphorylase from Thermotoga maritima in Caldicellulosiruptor bescii improves the phosphorolytic pathway and results in a dramatic increase in cellulolytic activity

Thermotoga maritima

Q9X2G3

746847

expression of a functional recombinant enzyme containing an N-terminal metal affinity fusion peptide in Escherichia coli. Catalytic properties of the natural enzyme are retained completely in the recombinant cellobiose phosphorylase

Cellulomonas uda

659742

expression of intein-tagged enzyme in Escherichia coli strain BL21, subcloning in Escherichia coli strain DH5alpha

Acetivibrio thermocellus

693340